LOCUS       sequence-212821-        8692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite (attR3/attR4) Gateway vector for expression in oomycetes. 
            Confers resistance to geneticin (G418). Contains additional cassette
            for constitutive expression of mTFP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Evangelisti E, Shenhav L, Yunusov T, Le Naour-Vernet M, Rink P, 
            Schornack S
  TITLE     Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched 
            Hyphae of an Oomycete Pathogen.
  JOURNAL   MBio. 2019 Oct 1;10(5). pii: mBio.01516-19. doi: 
            10.1128/mBio.01516-19.
  PUBMED    31575765
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-10-1"; volume: "10(5). pii: mBio.01516-19. doi"; pages: 
            " 10.1128/mBio.01516-19"
COMMENT     Sequence Label: pTORKm43GWC
FEATURES             Location/Qualifiers
     source          1..8692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(322..341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(323..341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(348..365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(348..364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(357..379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(505..960)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(642..663)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     854..873
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        987..1091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1092..1160,1161..1952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1310..1329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2123..2710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2612..2631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2864..2881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2998..3019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3034..3039,3040..3057,3058..3064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3072..3088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3077..3099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3096..3112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3096..3112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3133..3151
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3744..3760
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3745..3761
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3767..4477
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     protein_bind    5175..5298
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5339..5644)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5441..5460)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5986..6645)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6560..6579
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6699..6705,6706..6723,6724..6729))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6755..6878)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7492..8286
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEPARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7546..7565)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8156..8175
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tacgccgcct tagtaacaaa catgccaaaa gaaggaaagc tttcaccagc tttcaagttt
       61 aatcaatctt tgagagtcac aattgcaaga ataaatgaat ttttacaaca cagttcgatg
      121 cactgtgttt cgtcttgatg aaaattggac ggcaaatcga gtatcttaaa caaatctcgg
      181 cataggctgt ccattttgtg gtcgcctctt tgttggtagt ttgcagtagt ttcattagta
      241 gaagatctgc atcctcaatc tgctaaacgt cttgagcagc tatgagacga tccggggaat
      301 tggggcccga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      481 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
      541 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
      601 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
      661 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      721 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      781 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      841 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      901 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
      961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2641 tcgggtttcg ccacctctga cttgagcgtc gattttgtga tgctcgtcag gggggcggag
     2701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     3061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     3121 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtacctc tgatggacaa
     3181 agggtcgcct ctacgtaccc gcgacaatct cggcttttcc aatgtcgaca gctggcatat
     3241 cgcacgcgag gttaggcgtc ttcagccggc gtaaccacaa acctcgagtc aagtaccctt
     3301 tttcagacct taatttccgt gtccattccc tcgcgtttac acgaaggttt ctgagaaata
     3361 ccacatgtca cagagagaga gaagattgga gaaccttctc aacctgaccc gtgcagagcg
     3421 cttttgcgtc ctaccatccg ttaattatag caccaccacc acatgtctcg ccggaactct
     3481 cttatccgct ctttttgatc gtcttttttg cgccccatta aagacatctc ttgcacaggg
     3541 atcgtttctg attggccatt tcacttcctc ttttacatcc gaaaaccacc tgtttgtggg
     3601 gttgtgaagc cacaaccgga cgctcaaaaa gcctcgcccg actcgcccac gatcggaagg
     3661 ctcattctcc ttttcactct cacgtgccca agtcccaacc gactcttttt cgaccttcac
     3721 tctcaccgac aacgggcccc ccctcgaggt cgacggtatc gataccatgg tgagcaaggg
     3781 cgaggagacc acaatgggcg taatcaagcc cgacatgaag atcaagctga agatggaggg
     3841 caacgtgaat ggccacgcct tcgtgatcga gggcgagggc gagggcaagc cctacgacgg
     3901 caccaacacc atcaacctgg aggtgaagga gggagccccc ctgcccttct cctacgacat
     3961 tctgaccacc gcgttcgcct acggcaacag ggccttcacc aagtaccccg acgacatccc
     4021 caactacttc aagcagtcct tccccgaggg ctactcttgg gagcgcacca tgaccttcga
     4081 ggacaagggc atcgtgaagg tgaagtccga catctccatg gaggaggact ccttcatcta
     4141 cgagatacac ctcaagggcg agaacttccc ccccaacggc cccgtgatgc agaagaagac
     4201 caccggctgg gacgcctcca ccgagaggat gtacgtgcgc gacggcgtgc tgaagggcga
     4261 cgtcaagcac aagctgctgc tggagggcgg cggccaccac cgcgttgact tcaagaccat
     4321 ctacagggcc aagaaggcgg tgaagctgcc cgactatcac tttgtggacc accgcatcga
     4381 gatcctgaac cacgacaagg actacaacaa ggtgaccgtt tacgagagcg ccgtggcccg
     4441 caactccacc gacggcatgg acgagctgta caagtaggcc gaaggtcgag ctgtgtgcac
     4501 tgagtgcgaa gttgcagatt ttgtgtctat gcatttaata caatttcctt ttttccattt
     4561 tcgttgtttt tttttgtgaa agaacgattg ttttcaaaag aaggccgttt gtagaacaat
     4621 ttttttttat actagcattt ggtattcgag tgtgacgaat cggtgatggc gctttacaaa
     4681 tgaaatataa cgctgaacaa gcgcagtgac agctttattt tataacagag aataaagacc
     4741 aaagtagagt agactgatgg cggcttgaaa caaaactacg tgacttctaa tgcaaagaaa
     4801 accatacccc acgctactta gaattgtttc tctttggttg atgaccgcta tagtcgtatg
     4861 gagtgatccc tttttgtcaa tatttcgatt cttgctaaaa ccgtgactat tttgaggcaa
     4921 ctgtgtgtac ttaacatggt agaaatttct atgtttaagc cagctgacaa tcattcttta
     4981 tttatcgtaa accaaccagg taccgggccc attatacctc gtattcgatt aaatgataca
     5041 actcattgcc acaccgcaag acggcgtcac caccatagac agcctttcaa gcaccccctt
     5101 gtctatgagc tgccacgtca gctgccgctc ctaatatcca cccgcgtccc tattcccgtc
     5161 atatgccaag ctatcaactt tgtatagaaa agttgaacga gaaacgtaaa atgatataaa
     5221 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     5281 acatatccag tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt
     5341 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
     5401 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
     5461 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggggact ttatctgaca
     5521 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
     5581 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
     5641 gcatttcacc agcccctgtt ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg
     5701 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
     5761 tcattctgca tggttgtgct taccgaaccg gagatattga catcatatat gccttgagca
     5821 actgatagct gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt
     5881 tgacatactt cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa
     5941 atacgcatac tgttatctgg cttttagtaa gccggatcct ctagattacg ccccgccctg
     6001 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     6061 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     6121 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     6181 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     6241 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     6301 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     6361 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     6421 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     6481 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     6541 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     6601 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     6661 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
     6721 aagtgtaaag cctgggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt
     6781 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     6841 attttacgtt tctcgttcaa ctttattata catagttgat aattcacata tgcgtcaatg
     6901 caattgactg tgtcctcgat tatttctatt attaatatta tttccattaa aaaagaaatc
     6961 cagttcgtgg caaaataaga caaaggttta aataaaaatt tatcgttatt aaactcgtaa
     7021 cttaccttct tcatggctca acccaatctg tatatcgact tatttaattt ggttaacaaa
     7081 tcggttttcg tcgcaaatag gggatgacaa ctaaaatacc aatttgtgtc cttgcgacac
     7141 gtcaacaggt ggctgccaat atctcaggtt ttgagcacac aacgctgtga ttggctatct
     7201 gtagatcgct tcgagaactt tcggaccgac atatcagaac gcagtcatgt ccaatccagc
     7261 taacgattgg tcgaaaccgc acttcatcgc ttcatttcaa ccaattccca tctactaaaa
     7321 cgagcgtcgg aatagtctcg aacgtaaccc cacgtagtgg gtggcagcgc ccctcgtatc
     7381 tttcaatcgc cactcgagtc tcaagtttgc tcactttgaa attttccatc tggactacac
     7441 atcaaatcta actcaacttt tatcaattga aacacaaggg ccccgtttcg catgattgaa
     7501 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     7561 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7621 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     7681 ccagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7741 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     7801 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7861 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7921 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7981 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     8041 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     8101 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     8161 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     8221 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     8281 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     8341 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8401 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccc
     8461 cggatccccg gggtcgacta gaatacttag caattggtta gtttgaaaat tgtcaaagca
     8521 gagttttttc gacaataaaa tttattttct aaatatttga tttagcgttg acttaatttg
     8581 ttgattcggt tgtcatcttt tgtgaagatg cccgagagtc tagtaaatct caagcgaaac
     8641 atagtgagaa ggtcactatg gctggtttgc atgagtctgg gcctattgtg ct
//
