LOCUS       sequence-212831-        9084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, 
            Varjosalo M
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping
            of protein interactions and subcellular localizations.
  JOURNAL   Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2.
  PUBMED    29568061
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-22"; volume: "9(1):1188. doi"; pages: " 
            10.1038/s41467-018-03523-2"
COMMENT     Sequence Label: MAC_C_HXK1
FEATURES             Location/Qualifiers
     source          1..9084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    601..619
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    622..640
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     650..674
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    817..841
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3602..3626)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3673..3696
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3733..3756
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3787..3813
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3787..3813
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3814..4161,4162..4164,4165..4773)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4817..4834)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4823..5047
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5180..5199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5331..5378
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5386..6408
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5386..6408
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6538..6659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6575..6594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6629..6648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6708..6724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6708..6724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6721..6743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6732..6748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6756..6762,6763..6780,6781..6786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6801..6822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6939..6956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7110..7698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7190..7209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7869..8660,8661..8729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8730..8834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8909..8928)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      601 tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag
      661 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      721 gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc gagctcggat
      781 ccactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc
      841 tccaccatga tcgccgcgca gctcctggcc tattacttca cggagctgaa ggatgaccag
      901 gtcaaaaaga ttgacaagta tctgtatgcc atgcggctct ccgatgaaac tctcatagat
      961 atcatgactc gcttcaggaa ggagatgaag aatggcctct cccgggattt taatccaaca
     1021 gccacagtca agatgttgcc aacattcgta aggtccattc ctgatggctc tgaaaaggga
     1081 gatttcattg ccctggatct tggtgggtct tcctttcgaa ttctgcgggt gcaagtgaat
     1141 catgagaaaa accagaatgt tcacatggag tccgaggttt atgacacccc agagaacatc
     1201 gtgcacggca gtggaagcca gctttttgat catgttgctg agtgcctggg agatttcatg
     1261 gagaaaagga agatcaagga caagaagtta cctgtgggat tcacgttttc ttttccttgc
     1321 caacaatcca aaatagatga ggccatcctg atcacctgga caaagcgatt taaagcgagc
     1381 ggagtggaag gagcagatgt ggtcaaactg cttaacaaag ccatcaaaaa gcgaggggac
     1441 tatgatgcca acatcgtagc tgtggtgaat gacacagtgg gcaccatgat gacctgtggc
     1501 tatgacgacc agcactgtga agtcggcctg atcatcggca ctggcaccaa tgcttgctac
     1561 atggaggaac tgaggcacat tgatctggtg gaaggagacg aggggaggat gtgtatcaat
     1621 acagaatggg gagcctttgg agacgatgga tcattagaag acatccggac agagtttgac
     1681 agggagatag accggggatc cctcaaccct ggaaaacagc tgtttgagaa gatggtcagt
     1741 ggcatgtact tgggagagct ggttcgactg atcctagtca agatggccaa ggagggcctc
     1801 ttatttgaag ggcggatcac cccggagctg ctcacccgag ggaagtttaa caccagtgat
     1861 gtgtcagcca tcgaaaagaa taaggaaggc ctccacaatg ccaaagaaat cctgacccgc
     1921 ctgggagtgg agccgtccga tgatgactgt gtctcagtcc agcacgtttg caccattgtc
     1981 tcatttcgct cagccaactt ggtggctgcc acactgggcg ccatcttgaa ccgcctgcgt
     2041 gataacaagg gcacacccag gctgcggacc acggttggtg tcgacggatc tctttacaag
     2101 acgcacccac agtattcccg gcgtttccac aagactctaa ggcgcttggt gccagactcc
     2161 gatgtgcgct tcctcctctc ggagagtggc agcggcaagg gggctgccat ggtgacggcg
     2221 gtggcctacc gcttggccga gcagcaccgg cagatagagg agaccctggc tcatttccac
     2281 ctcaccaaag acatgctgct ggaggtgaag aagaggatgc gggccgagat ggagctgggg
     2341 ctgaggaagc agacgcacaa caatgccgtg gttaagatgc tgccctcctt cgtccggaga
     2401 actcccgacg ggaccgagaa tggtgacttc ttggccctgg atcttggagg aaccaatttc
     2461 cgtgtgctgc tggtgaaaat ccgtagtggg aaaaagagaa cggtggaaat gcacaacaag
     2521 atctacgcca ttcctattga aatcatgcag ggcactgggg aagagctgtt tgatcacatt
     2581 gtctcctgca tctctgactt cttggactac atggggatca aaggccccag gatgcctctg
     2641 ggcttcacgt tctcatttcc ctgccagcag acgagtctgg acgcgggaat cttgatcacg
     2701 tggacaaagg gttttaaggc aacagactgc gtgggccacg atgtagtcac cttactaagg
     2761 gatgcgataa aaaggagaga ggaatttgac ctggacgtgg tggctgtggt caacgacaca
     2821 gtgggcacca tgatgacctg tgcttatgag gagcccacct gtgaggttgg actcattgtt
     2881 gggaccggca gcaatgcctg ctacatggag gagatgaaga acgtggagat ggtggagggg
     2941 gaccaggggc agatgtgcat caacatggag tggggggcct ttggggacaa cgggtgtctg
     3001 gatgatatca ggacacacta cgacagactg gtggacgaat attccctaaa tgctgggaaa
     3061 caaaggtatg agaagatgat cagtggtatg tacctgggtg aaatcgtccg caacatctta
     3121 atcgacttca ccaagaaggg attcctcttc cgagggcaga tctctgagac gctgaagacc
     3181 cggggcatct ttgagaccaa gtttctctct cagatcgaga gtgaccgatt agcactgctc
     3241 caggtccggg ctatcctcca gcagctaggt ctgaatagca cctgcgatga cagtatcctc
     3301 gtcaagacag tgtgcggggt ggtgtccagg agggccgcac agctgtgtgg cgcaggcatg
     3361 gctgcggttg tggataagat ccgcgagaac agaggactgg accgtctgaa tgtgactgtg
     3421 ggagtggacg ggacactcta caagcttcat ccacacttct ccagaatcat gcaccagacg
     3481 gtgaaggaac tgtcaccaaa atgtaacgtg tccttcctcc tgtctgagga tggcagcggc
     3541 aagggggccg ccctcatcac ggccgtgggc gtgcggttac gcacagaggc aagcagcttg
     3601 gacccagctt tcttgtacaa agtggttgat atcaaggccg ccgacatcac cagcctgtac
     3661 aagaaagtgg ggtggagcca tccccagttc gagaagggcg gaggatctgg cggcggaagc
     3721 ggcggaggaa gctggtccca ccctcagttt gagaaaggca ccgagctggg cagcaccatg
     3781 gccagctacc cctacgacgt gcccgactac gccaaggaca acaccgtgcc cctgaagctg
     3841 atcgccctgc tggccaacgg cgagttccac tctggcgagc agctgggcga gacactgggc
     3901 atgagcagag ccgccatcaa caagcacatc cagaccctgc gggactgggg cgtggacgtg
     3961 ttcacagtgc ccggcaaggg ctacagcctg cccgagccca tccagctgct gaacgccaag
     4021 cagatcctgg gccagctgga tggcggatct gtggccgtgc tgcccgtgat cgacagcacc
     4081 aaccagtatc tgctcgaccg gatcggcgag ctgaagtccg gcgacgcctg tatcgccgag
     4141 taccagcagg ccggcagagg cggcaggggc agaaagtggt tcagcccctt cggcgccaac
     4201 ctgtacctga gcatgttctg gagactggaa cagggcccag ccgccgctat tggcctgagc
     4261 ctggtcatcg gcatcgtgat ggccgaggtg ctgcggaagc tgggcgccga caaagtgcgc
     4321 gtgaagtggc ccaacgacct gtatctgcag gaccggaagc tggccggcat cctggtggaa
     4381 ctgaccggca aaacaggcga cgccgcccag atcgtgatcg gcgctggcat caacatggcc
     4441 atgcggcggg tggaagagag cgtggtcaac cagggctgga tcaccctgca ggaagccggc
     4501 atcaacctgg accggaacac cctggccgcc atgctgatca gagagctgag agccgccctg
     4561 gaactgttcg agcaggaagg cctggccccc tacctgagca gatgggagaa gctggacaac
     4621 ttcatcaacc ggcccgtgaa actgatcatc ggcgacaaag agatcttcgg catcagccgg
     4681 ggcatcgaca agcagggggc cctgctcctg gaacaggacg gcatcatcaa gccctggatg
     4741 ggcggcgaga tcagcctgcg gagcgccgag aagtgatgac tcgagtctag agggcccgtt
     4801 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4861 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4921 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4981 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5041 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5101 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5161 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5221 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5281 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta
     5341 ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc
     5401 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     5461 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     5521 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     5581 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     5641 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     5701 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     5761 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     5821 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     5881 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     5941 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     6001 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     6061 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     6121 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     6181 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     6241 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     6301 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     6361 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga
     6421 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6481 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6661 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6721 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6781 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7981 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8161 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8461 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8881 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8941 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     9001 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     9061 ttgcgctgct tcgcgatgta cggg
//
