LOCUS       sequence-212847-       15526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation and expression of Csy4 and cogGFP proteins in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Liang Y, Richardson S, Yan J, Benites VT, Cheng-Yue C, Tran T, 
            Mortimer J, Mukhopadhyay A, Keasling JD, Scheller HV, Loque D
  TITLE     Endoribonuclease-Based Two-Component Repressor Systems for Tight 
            Gene Expression Control in Plants.
  JOURNAL   ACS Synth Biol. 2017 May 19;6(5):806-816. doi: 
            10.1021/acssynbio.6b00295. Epub 2017 Feb 8.
  PUBMED    28094975
REFERENCE   2  (bases 1 to 15526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00295"; journalName: "ACS Synth Biol"; date: 
            "2017-05-19- 19"; volume: "6"; issue: "5"; pages: "806-816"
COMMENT     Sequence Label: pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)
FEATURES             Location/Qualifiers
     source          1..15526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..153
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    318..339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(354..359,360..377,378..384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    392..408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     397..419
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     416..432
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     416..432
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        735..1080
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1023..1043
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1111..1135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1144..1164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1736..1756)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      1780..1980
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4949..4969
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4973..4992
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             join(5002..5004,5005..5007,5008..5718)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5047..5068)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5308..5327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5655..5676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5720..5744)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5842..6483
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(6491..6508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6491..6507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6500..6522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(7089..7341)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(7537..8331)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7648..7667)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8258..8277
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8361..8741)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    9113..9137
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9658..10449
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10695..11283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11184..11203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11437..11454
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11469..11609
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11535..11554)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11695..11717
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11953..12147
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12213..13286)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13715..14344)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
       61 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
      121 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
      181 gaaacgacaa tctgatccaa gctcaagctg ctctagccaa tacgcaaacc gcctctcccc
      241 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      301 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      361 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      421 aacagctatg acatgattac gaattcgagc tcggtacccg gggatcctct agagggcccg
      481 ggacgtccgc ggagatctac gcgtgtcgac tcgagcggcc gccagtgtga tggatatctg
      541 cagaattcag gcatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
      601 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc
      661 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
      721 gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
      781 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
      841 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
      901 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      961 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     1021 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
     1081 cgggggactc ctagggctag cctgttatca acaagtttgt acaaaaaagc aggcttcatg
     1141 gctccaaaaa agaaaagaaa ggttgctagc gaccattact tagatattag attgagacca
     1201 gacccagagt tccctccagc acagttgatg tcagtgttat tcggaaaatt gcaccaagct
     1261 ttggttgcac aaggtggaga tagaattggt gtgagttttc ctgatttgga cgagtctagg
     1321 tcaagattgg gagaaaggtt aagaatccat gcttcagcag atgaccttag ggctttgtta
     1381 gcaagacctt ggttagaggg tcttagagat catttgcagt tcggagaacc agctgttgtg
     1441 ccacacccta caccatatag acaagtttct agagtgcagg caaaatcaaa tcctgagaga
     1501 cttagaagga gattgatgag gagacacgac ttatctgaag aggaagctag gaagagaata
     1561 cctgataccg ttgctagagc acttgacttg ccatttgtga ctttgagaag tcaatccact
     1621 ggtcagcatt ttaggttatt cattagacac ggaccacttc aagttactgc agaggaggga
     1681 ggattcacct gttatggatt gagtaaggga ggattcgtgc cttggttctg acacccaact
     1741 tttctataca aagttgactg actaactagg atgagctaag ctagctatat catcaattta
     1801 tgtattacac ataatatcgc actcagtctt tcatctacgg caatgtacca gctgatataa
     1861 tcagttattg aaatatttct gaatttaaac ttgcatcaat aaatttatgt ttttgcttgg
     1921 actataatac ctgacttgtt attttatcaa taaatattta aactatattt ctttcaagat
     1981 gggaattaac atctacaaat tgccttttct tatcgaccat gtaccccggg taccaagctt
     2041 ctcgagcgga atgagagacg agagcaatgt gctaagagaa gagattggga agagagaaga
     2101 gaagataaag gaaacggaaa agcatatgga ggagcttcac atggagcaag tgaggctgag
     2161 aagacggtca agtgagctta cggaagaagt ggaaaggacg agagtgtctg catcggaaat
     2221 ggctgagcag aaaagagaag ctataagaca gctttgtatg tctcttgacc attacagaga
     2281 tgggtacgac agactttgga gagttgttgc aggacataag agtaagagag tagtggtctt
     2341 atcaacttga agtgtaagaa caatgagtca atgactacgt gcaggacatt ggacataccg
     2401 tgtgttcttt tggattgaaa tgttgtttcg aagggctgtt agttgatgtt gaaaataggt
     2461 tgaagttgaa taatgcatgt tgatatagta aatatcaatg gtaatatttt ctcatttccc
     2521 aaaactcaaa tgatatcatt taactataaa ctaacgtaaa ctgttgacaa tacacttatg
     2581 gttaaaaatt tggagtcttg ttttagtata cgtatcacca ccgcacggtt tcaaaaccac
     2641 ataattgtaa atgttattgg aaaatagaac tcgcaatacg tattgtattt tggtaaacat
     2701 agctctaagc ctctaatata taagctctca acaattctgg ctaatggtcc caagtaagaa
     2761 aagcccatgt attgtaaggt catgatctca aaaacgaggg tgaggtggaa tactaacatg
     2821 aggagaaagt aaggtgacaa atttttgggg caatagtggt ggatatggtg gggaggtagg
     2881 tagcatcatt tctccaagtc gctgtctttc gtggtaatgg taggtgtgtc tctctttata
     2941 ttatttatta ctactcattg taaatttctt ttttctacaa tttgtttctg actccaaaat
     3001 acgtcacaaa tataatacta ggcaaataat tattttatta taagtcaata gagtggttgt
     3061 tgtaaaattg attttttgat attgaaagag ttcatggacg gatgtgtatg cgccaaatgg
     3121 taagcccttg tactgtgccg cgcgtatatt ttaaccacca ctagttgttt ctctttttca
     3181 aaaaacacaa aaaaaaaata atttgttttc ttaacggcgt caaatctgac ggcgtctcaa
     3241 tacgttcaat ttttttcttt ctttcacatg gtttctcata gctttgcatt gaccataggt
     3301 aaagggataa ggataatggt tttttctctt gtttgtttta tccttattat tcaaaaagga
     3361 taaaaaaaca gtgatattta gatttctttg attaaaaaag tcattgaaat tcatatttga
     3421 ttttttgcta aatgtcaaca cagagacaca aacgtaatgc actgtcgcca atattcatgg
     3481 atcatgacaa taaatatcac tagaataatt aaaaatcagt agaatgcaaa caaagcattt
     3541 tctaagtaaa acagtctttt atattcacgt aattggaatt tccttttttt ttttttgtcg
     3601 taattggaat ttcctttatc aaacccaaag tccaaaacaa tcggcaatgt tttgcaaaat
     3661 gttcaaaact attggcgggt tggtctatcc gaattgaaga tcttttctcc atatgataga
     3721 ccaacgaaat tcggcatacg tgtttttttt tttgttttga aaacccttta aacaacctta
     3781 attcaaaata ctaatgtaac tttattgaac gtgcatctaa aaattttgaa ctttgctttt
     3841 gagaaataat caatgtacca ataaagaaga tgtagtacat acattataat taaatacaaa
     3901 aaaggaatca ccatatagta catggtagac aatgaaaaac tttaaaacat atacaatcaa
     3961 taatactctt tgtgcataac tttttttgtc gtctcgagtt tatatttgag tacttataca
     4021 aactattaga ttacaaactg tgctcagata cattaagtta atcttatata caagagcact
     4081 cgagtgttgt ccttaagtta atcttaagat atcttgaggt aaatagaaat agttgactcg
     4141 tttttatctt cttctttttt taccatgagc aaaaaagatg aaataagttc aaaacgtgac
     4201 gaatctatat gttactactt agtatgtgtc aatcattaaa tcgggaaaac ttcatcattt
     4261 caggagtatt acaaaactcc taagagtgag aacgactaca tagtacatat tttgataaaa
     4321 gacttgaaaa cttgctaaaa cgaatttgcg aaaatataat catacaagtg ccagtgattt
     4381 tgatcgaatt attcatagct ttgtaggatg aacttaatta aataatatct cacaaaagta
     4441 ttgacagtaa cctagtacta tactatctat gttagaatat gattatgata taatttatcc
     4501 cctcacttat tcatatgatt tttgaagcaa ctactttcgt ttttttaaca ttttcttttg
     4561 ttggttattg ttaatgagca tatttagtcg tttcttaatt ccactgaaat agaaaataca
     4621 aagagaactt tagttaatag atatgaacat aatctcacat cctcctccta ccttcaccaa
     4681 acacttttac atacactttg tggtctttct ttacctacca ccatcaacaa caacaccaag
     4741 ccccactcac acacacgcaa tcacgttaaa tttaacgccg tttattatct catcattcac
     4801 caactcccac gtacctaacg ccgtttacct tttgccgttg gtcctcattt ctcaaaccaa
     4861 ccaaacctct ccctcttata aaatcctctc tcccttcttt atttcttcct cagcagcttc
     4921 ttctgctttc aattactctc gcctaggaca actttgtata ataaagttgg ccgttcactg
     4981 ccgtataggc agctaagaaa tatggtgagc aagggcgagg agctgttcac cggggtggtg
     5041 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     5101 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5161 ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcagc
     5221 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5281 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5341 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5401 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5461 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     5521 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5581 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5641 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactcacggc
     5701 atggacgagc tgtacaagga cccagctttc ttgtacaaag tggttgataa cagcgctgag
     5761 ctctagcacg tgcgggcctg aattccagca cactggcggc cgttactagt ggatccgagc
     5821 tcggtaccaa gcttgatgca gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca
     5881 agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg
     5941 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
     6001 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
     6061 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
     6121 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
     6181 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat
     6241 gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa
     6301 tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag
     6361 aatccttgtc agattctaat cattgcttta taattatagt tatactcatg gatttgtagt
     6421 tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat
     6481 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6541 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6601 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttgc
     6661 caatcgaggc cgaccaaccg caagcgttgt cagtgttgca aagcgctctg tgtgggccta
     6721 ctttaattgc ttccagtgtt aaattggcga aaggcaataa tatcgcaaaa tattgtgttg
     6781 taaaatgtaa ttatgtttta atttcatgga aatgtttgag cataattttt attaatgtac
     6841 taaattactg ttttgttaaa tgcaattttg ctttctcggg attttaatat caaaatctat
     6901 ttagaaatac acaatatttt gttgcaggct tgctggagaa tcgatctgct atcataaaaa
     6961 ttacaaaaaa attttatttg cctcaattat tttaggattg gtattaagga cgcttaaatt
     7021 atttgtcggg tcactacgca tcattgtgat tgagaagatc agcgatacga aatattcgta
     7081 gtactatcga taatttattt gaaaattcat aagaaaagca aacgttacat gaattgatga
     7141 aacaatacaa agacagataa agccacgcac atttaggata ttggccgaga ttactgaata
     7201 ttgagtaaga tcacggaatt tctgacagga gcatgtcttc aattcagccc aaatggcagt
     7261 tgaaatactc aaaccgcccc atatgcagga gcggatcatt cattgtttgt ttggttgcct
     7321 ttgccaacat gggagtccaa ggtttcaggg aagctggaat tccggggtgg gcgaagaact
     7381 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     7441 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     7501 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     7561 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     7621 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     7681 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     7741 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     7801 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     7861 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     7921 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     7981 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     8041 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     8101 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct ggagttcatt
     8161 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     8221 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     8281 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     8341 tccggggaat tcgctagagt cgatttggtg tatcgagatt ggttatgaaa ttcagatgct
     8401 agtgtaatgt attggtaatt tgggaagata taataggaag caaggctatt tatccatttc
     8461 tgaaaaggcg aaatggcgtc accgcgagcg tcacgcgcat tccgttcttg ctgtaaagcg
     8521 ttgtttggta cacttttgac tagcgaggct tggcgtgtca gcgtatctat tcaaaagtcg
     8581 ttaatggctg cggatcaaga aaaagttgga atagaaacag aatacccgcg aaattcaggc
     8641 ccggttgcca tgtcctacac gccgaaataa acgaccaaat tagtagaaaa ataaaaactg
     8701 actcggatac ttacgtcacg tcttgcgcac tgatttgaaa aatctcaata taaacaaaga
     8761 cggccacaag aaaaaaccaa aacaccgata ttcattaatc ttatctagtt tctcaaaaaa
     8821 attcatatct tccacacgtg aaaatgccaa tttctcagac ctacctcggc tctgcgaagg
     8881 cccccgctgg tatcaaaagt ttttatttca tccgacatgg cgcgaccgac ctcaacgaga
     8941 aggaaattgt cgtgaacggt gagaagctct ggggcgtgca aggttccgga acgaacatcg
     9001 gtctcaatgc aaaaggggaa cgccaggctc tgttggcccc tcgaaattcg gcgttaattc
     9061 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     9121 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     9181 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     9241 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     9301 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     9361 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     9421 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     9481 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     9541 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     9601 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     9661 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     9721 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     9781 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     9841 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     9901 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     9961 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
    10021 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
    10081 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
    10141 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
    10201 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
    10261 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
    10321 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
    10381 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
    10441 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
    10501 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
    10561 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
    10621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10801 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    10861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11341 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11401 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11461 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
    11521 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
    11581 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
    11641 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
    11701 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
    11761 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
    11821 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
    11881 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
    11941 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
    12001 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
    12061 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
    12121 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
    12181 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
    12241 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
    12301 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
    12361 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
    12421 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
    12481 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
    12541 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
    12601 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
    12661 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
    12721 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
    12781 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
    12841 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
    12901 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
    12961 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
    13021 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
    13081 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
    13141 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
    13201 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
    13261 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
    13321 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    13381 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    13441 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    13501 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    13561 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    13621 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    13681 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    13741 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    13801 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    13861 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    13921 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    13981 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    14041 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    14101 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    14161 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    14221 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    14281 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    14341 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    14401 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    14461 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    14521 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    14581 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    14641 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    14701 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    14761 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    14821 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    14881 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    14941 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    15001 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    15061 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    15121 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    15181 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    15241 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    15301 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    15361 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    15421 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    15481 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaag
//
