LOCUS       sequence-212854-       15492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct to express dCas9 fused with an inactive 
            catalytic domain of Tet1 (H1672Y, D1674A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, 
            Young RA, Jaenisch R
  TITLE     Editing DNA Methylation in the Mammalian Genome.
  JOURNAL   Cell. 2016 Sep 22;167(1):233-247.e17. doi: 
            10.1016/j.cell.2016.08.056.
  PUBMED    27662091
REFERENCE   2  (bases 1 to 15492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08"; journalName: "Cell"; date: "2016-09-22- 
            22"; volume: "167"; issue: "1"; pages: "233-247.e17"
COMMENT     Sequence Label: Fuw-dCas9-Tet1CD_IM
FEATURES             Location/Qualifiers
     source          1..15492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        102..481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        483..681
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     636..656
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             699..879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    926..1051
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1544..1777
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1962..2006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2304..2421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2486..3697
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3534..3553
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3748..3774,3775..3777,3778..6264,6265..6267,
                     6268..7851)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7864..7884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7888..7908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7912..7932
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    10120..10708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10173..10193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10591..10602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10711..10727)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10712..10728)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11233..11413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11439..11456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11445..11669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11715..12143
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11802..11821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12012..12033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12152..12172)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12196..12406
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12218..12414
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12265..12400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12327..12346
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12462..12509
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12528..12902
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13032..13153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13069..13088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13123..13142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13202..13218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13202..13218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13215..13237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13226..13242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13250..13256,13257..13274,13275..13280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13295..13316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13433..13450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13604..14192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13684..13703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14363..15154,15155..15223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14986..15005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15224..15328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15403..15422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
       61 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
      121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      301 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      361 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      421 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      481 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      601 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      661 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
      721 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      781 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      841 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      901 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      961 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1021 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1081 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1141 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1201 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1261 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1321 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1381 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1441 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1501 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1561 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1621 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1681 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1741 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1801 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1861 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1921 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1981 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2041 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2101 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2161 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2221 acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga
     2281 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2341 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2401 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
     2461 attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc
     2521 gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat
     2581 ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc
     2641 cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc
     2701 tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga
     2761 tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
     2821 gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac
     2881 ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg
     2941 ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc
     3001 cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg
     3061 aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg
     3121 caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg
     3181 ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt
     3241 tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt
     3301 tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg
     3361 gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc
     3421 gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata
     3481 agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct
     3541 ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc
     3601 ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc
     3661 cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgact
     3721 ctagaagtta acctcgaggg cgcgcccatg gacaagaagt attctatcgg actggccatc
     3781 gggactaata gcgtcgggtg ggccgtgatc actgacgagt acaaggtgcc ctctaagaag
     3841 ttcaaggtgc tcgggaacac cgaccggcat tccatcaaga aaaatctgat cggagctctc
     3901 ctctttgatt caggggagac cgctgaagca acccgcctca agcggactgc tagacggcgg
     3961 tacaccagga ggaagaaccg gatttgttac cttcaagaga tattctccaa cgaaatggca
     4021 aaggtcgacg acagcttctt ccataggctg gaagaatcat tcctcgtgga agaggataag
     4081 aagcatgaac ggcatcccat cttcggtaat atcgtcgacg aggtggccta tcacgagaaa
     4141 tacccaacca tctaccatct tcgcaaaaag ctggtggact caaccgacaa ggcagacctc
     4201 cggcttatct acctggccct ggcccacatg atcaagttca gaggccactt cctgatcgag
     4261 ggcgacctca atcctgacaa tagcgatgtg gataaactgt tcatccagct ggtgcagact
     4321 tacaaccagc tctttgaaga gaaccccatc aatgcaagcg gagtcgatgc caaggccatt
     4381 ctgtcagccc ggctgtcaaa gagccgcaga cttgagaatc ttatcgctca gctgccgggt
     4441 gaaaagaaaa atggactgtt cgggaacctg attgctcttt cacttgggct gactcccaat
     4501 ttcaagtcta atttcgacct ggcagaggat gccaagctgc aactgtccaa ggacacctat
     4561 gatgacgatc tcgacaacct cctggcccag atcggtgacc aatacgccga ccttttcctt
     4621 gctgctaaga atctttctga cgccatcctg ctgtctgaca ttctccgcgt gaacactgaa
     4681 atcaccaagg cccctctttc agcttcaatg attaagcggt atgatgagca ccaccaggac
     4741 ctgaccctgc ttaaggcact cgtccggcag cagcttccgg agaagtacaa ggaaatcttc
     4801 tttgaccagt caaagaatgg atacgccggc tacatcgacg gaggtgcctc ccaagaggaa
     4861 ttttataagt ttatcaaacc tatccttgag aagatggacg gcaccgaaga gctcctcgtg
     4921 aaactgaatc gggaggatct gctgcggaag cagcgcactt tcgacaatgg gagcattccc
     4981 caccagatcc atcttgggga gcttcacgcc atccttcggc gccaagagga cttctacccc
     5041 tttcttaagg acaacaggga gaagattgag aaaattctca ctttccgcat cccctactac
     5101 gtgggacccc tcgccagagg aaatagccgg tttgcttgga tgaccagaaa gtcagaagaa
     5161 actatcactc cctggaactt cgaagaggtg gtggacaagg gagccagcgc tcagtcattc
     5221 atcgaacgga tgactaactt cgataagaac ctccccaatg agaaggtcct gccgaaacat
     5281 tccctgctct acgagtactt taccgtgtac aacgagctga ccaaggtgaa atatgtcacc
     5341 gaagggatga ggaagcccgc attcctgtca ggcgaacaaa agaaggcaat tgtggacctt
     5401 ctgttcaaga ccaatagaaa ggtgaccgtg aagcagctga aggaggacta tttcaagaaa
     5461 attgaatgct tcgactctgt ggagattagc ggggtcgaag atcggttcaa cgcaagcctg
     5521 ggtacctacc atgatctgct taagatcatc aaggacaagg attttctgga caatgaggag
     5581 aacgaggaca tccttgagga cattgtcctg actctcactc tgttcgagga ccgggaaatg
     5641 atcgaggaga ggcttaagac ctacgcccat ctgttcgacg ataaagtgat gaagcaactt
     5701 aaacggagaa gatataccgg atggggacgc cttagccgca aactcatcaa cggaatccgg
     5761 gacaaacaga gcggaaagac cattcttgat ttccttaaga gcgacggatt cgctaatcgc
     5821 aacttcatgc aacttatcca tgatgattcc ctgaccttta aggaggacat ccagaaggcc
     5881 caagtgtctg gacaaggtga ctcactgcac gagcatatcg caaatctggc tggttcaccc
     5941 gctattaaga agggtattct ccagaccgtg aaagtcgtgg acgagctggt caaggtgatg
     6001 ggtcgccata aaccagagaa cattgtcatc gagatggcca gggaaaacca gactacccag
     6061 aagggacaga agaacagcag ggagcggatg aaaagaattg aggaagggat taaggagctc
     6121 gggtcacaga tccttaaaga gcacccggtg gaaaacaccc agcttcagaa tgagaagctc
     6181 tatctgtact accttcaaaa tggacgcgat atgtatgtgg accaagagct tgatatcaac
     6241 aggctctcag actacgacgt ggacgccatc gtccctcaga gcttcctcaa agacgactca
     6301 attgacaata aggtgctgac tcgctcagac aagaaccggg gaaagtcaga taacgtgccc
     6361 tcagaggaag tcgtgaaaaa gatgaagaac tattggcgcc agcttctgaa cgcaaagctg
     6421 atcactcagc ggaagttcga caatctcact aaggctgaga ggggcggact gagcgaactg
     6481 gacaaagcag gattcattaa acggcaactt gtggagactc ggcagattac taaacatgtc
     6541 gcccaaatcc ttgactcacg catgaatacc aagtacgacg aaaacgacaa acttatccgc
     6601 gaggtgaagg tgattaccct gaagtccaag ctggtcagcg atttcagaaa ggactttcaa
     6661 ttctacaaag tgcgggagat caataactat catcatgctc atgacgcata tctgaatgcc
     6721 gtggtgggaa ccgccctgat caagaagtac ccaaagctgg aaagcgagtt cgtgtacgga
     6781 gactacaagg tctacgacgt gcgcaagatg attgccaaat ctgagcagga gatcggaaag
     6841 gccaccgcaa agtacttctt ctacagcaac atcatgaatt tcttcaagac cgaaatcacc
     6901 cttgcaaacg gtgagatccg gaagaggccg ctcatcgaga ctaatgggga gactggcgaa
     6961 atcgtgtggg acaagggcag agatttcgct accgtgcgca aagtgctttc tatgcctcaa
     7021 gtgaacatcg tgaagaaaac cgaggtgcaa accggaggct tttctaagga atcaatcctc
     7081 cccaagcgca actccgacaa gctcattgca aggaagaagg attgggaccc taagaagtac
     7141 ggcggattcg attcaccaac tgtggcttat tctgtcctgg tcgtggctaa ggtggaaaaa
     7201 ggaaagtcta agaagctcaa gagcgtgaag gaactgctgg gtatcaccat tatggagcgc
     7261 agctccttcg agaagaaccc aattgacttt ctcgaagcca aaggttacaa ggaagtcaag
     7321 aaggacctta tcatcaagct cccaaagtat agcctgttcg aactggagaa tgggcggaag
     7381 cggatgctcg cctccgctgg cgaacttcag aagggtaatg agctggctct cccctccaag
     7441 tacgtgaatt tcctctacct tgcaagccat tacgagaagc tgaaggggag ccccgaggac
     7501 aacgagcaaa agcaactgtt tgtggagcag cataagcatt atctggacga gatcattgag
     7561 cagatttccg agttttctaa acgcgtcatt ctcgctgatg ccaacctcga taaagtcctt
     7621 agcgcataca ataagcacag agacaaacca attcgggagc aggctgagaa tatcatccac
     7681 ctgttcaccc tcaccaatct tggtgcccct gccgcattca agtacttcga caccaccatc
     7741 gaccggaaac gctatacctc caccaaagaa gtgctggacg ccaccctcat ccaccagagc
     7801 atcaccggac tttacgaaac tcggattgac ctctcacagc tcggagggga tgagggagct
     7861 gatccaaaaa agaagagaaa ggtagaccca aagaaaaaac gaaaagtaga tccgaaaaag
     7921 aagaggaagg tgggatccct gcccacctgc agctgtcttg atcgagttat acaaaaagac
     7981 aaaggcccat attatacaca ccttggggca ggaccaagtg ttgctgctgt cagggaaatc
     8041 atggagaata ggtatggtca aaaaggaaac gcaataagga tagaaatagt agtgtacacc
     8101 ggtaaagaag ggaaaagctc tcatgggtgt ccaattgcta agtgggtttt aagaagaagc
     8161 agtgatgaag aaaaagttct ttgtttggtc cggcagcgta caggccacca ctgtccaact
     8221 gctgtgatgg tggtgctcat catggtgtgg gatggcatcc ctcttccaat ggccgaccgg
     8281 ctatacacag agctcacaga gaatctaaag tcatacaatg ggcaccctac cgacagaaga
     8341 tgcaccctca atgaaaatcg tacctgtaca tgtcaaggaa ttgatccaga gacttgtgga
     8401 gcttcattct cttttggctg ttcatggagt atgtacttta atggctgtaa gtttggtaga
     8461 agcccaagcc ccagaagatt tagaattgat ccaagctctc ccttacatga aaaaaacctt
     8521 gaagataact tacagagttt ggctacacga ttagctccaa tttataagca gtatgctcca
     8581 gtagcttacc aaaatcaggt ggaatatgaa aatgttgccc gagaatgtcg gcttggcagc
     8641 aaggaaggtc gacccttctc tggggtcact gcttgcctgg acttctgtgc tcatccctac
     8701 agggccattc acaacatgaa taatggaagc actgtggttt gtaccttaac tcgagaagat
     8761 aaccgctctt tgggtgttat tcctcaagat gagcagctcc atgtgctacc tctttataag
     8821 ctttcagaca cagatgagtt tggctccaag gaaggaatgg aagccaagat caaatctggg
     8881 gccatcgagg tcctggcacc ccgccgcaaa aaaagaacgt gtttcactca gcctgttccc
     8941 cgttctggaa agaagagggc tgcgatgatg acagaggttc ttgcacataa gataagggca
     9001 gtggaaaaga aacctattcc ccgaatcaag cggaagaata actcaacaac aacaaacaac
     9061 agtaagcctt cgtcactgcc aaccttaggg agtaacactg agaccgtgca acctgaagta
     9121 aaaagtgaaa ccgaacccca ttttatctta aaaagttcag acaacactaa aacttattcg
     9181 ctgatgccat ccgctcctca cccagtgaaa gaggcatctc caggcttctc ctggtccccg
     9241 aagactgctt cagccacacc agctccactg aagaatgacg caacagcctc atgcgggttt
     9301 tcagaaagaa gcagcactcc ccactgtacg atgccttcgg gaagactcag tggtgccaat
     9361 gctgcagctg ctgatggccc tggcatttca cagcttggcg aagtggctcc tctccccacc
     9421 ctgtctgctc ctgtgatgga gcccctcatt aattctgagc cttccactgg tgtgactgag
     9481 ccgctaacgc ctcatcagcc aaaccaccag ccctccttcc tcacctctcc tcaagacctt
     9541 gcctcttctc caatggaaga agatgagcag cattctgaag cagatgagcc tccatcagac
     9601 gaacccctat ctgatgaccc cctgtcacct gctgaggaga aattgcccca cattgatgag
     9661 tattggtcag acagtgagca catctttttg gatgcaaata ttggtggggt ggccatcgca
     9721 cctgctcacg gctcggtttt gattgagtgt gcccggcgag agctgcacgc taccactcct
     9781 gttgagcacc ccaaccgtaa tcatccaacc cgcctctccc ttgtctttta ccagcacaaa
     9841 aacctaaata agccccaaca tggttttgaa ctaaacaaga ttaagtttga ggctaaagaa
     9901 gctaagaata agaaaatgaa ggcctcagag caaaaagacc aggcagctaa tgaaggtcca
     9961 gaacagtcct ctgaagtaaa tgaattgaac caaattcctt ctcataaagc attaacatta
    10021 acccatgaca atgttgtcac cgtgtcccct tatgctctca cacacgttgc ggggccctat
    10081 aaccattggg tctgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
    10141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    10201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    10261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    10321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    10381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    10441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    10501 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    10561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    10621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    10681 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
    10741 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
    10801 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
    10861 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
    10921 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    10981 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
    11041 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
    11101 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    11161 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
    11221 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    11281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    11341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    11401 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
    11461 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    11521 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    11581 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    11641 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    11701 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
    11761 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
    11821 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
    11881 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
    11941 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
    12001 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
    12061 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
    12121 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
    12181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    12241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    12301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    12361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    12421 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
    12481 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
    12541 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
    12601 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
    12661 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
    12721 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
    12781 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
    12841 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
    12901 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    12961 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    13021 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    13081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    13141 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
    13201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    13261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    13321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    13381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    13441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    13501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    13561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    13621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    13681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    13741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    13801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    13861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    13921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    13981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    14041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    14101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    14161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    14221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    14281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    14341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    14401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    14461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    14521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    14581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    14641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    14701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    14761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    14821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    14881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    14941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    15001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    15061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    15121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    15181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    15241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    15301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    15361 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    15421 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    15481 gagcaaaatt ta
//
