LOCUS       sequence-212856-        7642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An entry vector with cas9 -2A-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q
  TITLE     Neutrophil-specific knockout demonstrates a role for mitochondria in
            regulating neutrophil motility in zebrafish.
  JOURNAL   Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 
            10.1242/dmm.033027.
  PUBMED    29590639
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2018-03-28"; volume: "11(3). pii: dmm.033027. doi"; 
            pages: " 10.1242/dmm.033027"
COMMENT     Sequence Label: pME-cas9-2A-mCherry
FEATURES             Location/Qualifiers
     source          1..7642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     670..686
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        696..714
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      752..761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             758..4858
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             4771..4779
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4874..4894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4904..4960
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4961..5671
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5106..5124)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5374..5393
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5673..5689)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5673..5689)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5720..5740)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5720..5738)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(5767..5866)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(5884..5902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5907..5923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5907..5923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6025..6044)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6036..6845
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6103..6122)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6992..7580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7481..7500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagacttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
      721 tgggtaccgg gccccccctc gaggtcgact cgccaccatg gataagaagt atagcatcgg
      781 cctggatatt ggaactaact ccgtgggttg ggcagtgatt acagacgact acaaggtccc
      841 tagcaagaaa tttaaggtgc tgggtaacac cgacaggcac agcatcaaga aaaatctgat
      901 tggagccctg ctgttcggtt ctggagagac tgccgaagca acacgcctga aaagaacagc
      961 aagaaggcgc tataccagaa ggaagaatag aatctgttac ctgcaggaga ttttctctaa
     1021 cgaaatggct aaggtggacg attcattctt tcataggctg gaggaaagtt tcctggtcga
     1081 ggaagataag aaacacgagc gccatcctat ctttggaaac attgtggacg aggtcgccta
     1141 tcacgaaaaa tacccaacca tctatcatct gcgcaagaaa ctggctgact ctactgataa
     1201 agccgacctg agactgatct atctggctct ggcccacatg attaagttca ggggtcattt
     1261 tctgatcgag ggcgatctga accccgacaa ttccgatgtg gacaagctgt tcatccagct
     1321 ggtccagatt tacaatcagc tgtttgagga aaaccctatt aatgcttcca gagtggacgc
     1381 aaaagctatc ctgtcagcca ggctgtccaa gtcacgcaga ctggagaacc tgattgcaca
     1441 gctgcccgga gaaaagagga acggtctgtt tggaaatctg atcgctctga gtctgggcct
     1501 gactcctaac ttcaaaagca attttgatct ggctgaggac gccaaactgc agctgtcaaa
     1561 ggacacatat gacgatgacc tggataacct gctggcacag atcggagatc agtacgctga
     1621 cctgttcctg gctgccaaaa atctgtccga cgcaatcctg ctgtcagata ttctgagagt
     1681 gaacagcgag attacaaaag cacctctgag tgccagcatg atcaagagat atgacgagca
     1741 ccatcaggat ctgaccctgc tgaaggctct ggtcaggcag cagctgccag agaagtacaa
     1801 ggaaattttc tttgatcagt ccaagaacgg ctacgccggt tatatcgacg gaggcgcatc
     1861 acaggaggaa ttctacaagt ttatcaaacc tattctggag aagatggacg gaactgagga
     1921 actgctggtg aaactgaata gagaggacct gctgaggaag cagcgcacat ttgataacgg
     1981 ttccatccca caccagattc atctgggaga gctgcacgct atcctgaggc gccaggaaga
     2041 cttctacccc tttctgaaag ataaccgcga gaagatcgaa aaaattctga ccttcagaat
     2101 cccttactat gtgggtccac tggctcgcgg aaacagcaga tttgcctgga tgactcgcaa
     2161 atccgaggaa accattactc cttggaactt cgaggaagtg gtcgataagg gcgcctctgc
     2221 acagtccttc atcgagagaa tgactaattt tgacaaaaac ctgcccaatg agaaagtgct
     2281 gcctaagcac tccctgctgt acgagtattt cactgtctat aacgaactga caaaggtgaa
     2341 atacgtcacc gagggcatga gaaagccagc cttcctgtca ggagagcaga agaaagcaat
     2401 cgtggatctg ctgtttaaaa ccaacaggaa agtgactgtc aagcagctga aggaggacta
     2461 cttcaagaaa attgaatgct tcgattccgt ggagatcagc ggagtcgaag acagatttaa
     2521 cgcaagcctg ggcgcttacc acgatctgct gaagatcatt aaggataaag acttcctgga
     2581 caacgaggaa aatgaggata tcctggaaga cattgtgctg acactgaccc tgtttgagga
     2641 cagaggaatg atcgaggaaa gactgaaaac ctatgctcat ctgttcgatg acaaggtgat
     2701 gaaacagctg aagagaaggc gctacactgg ctggggtaga ctgagcagga agctgatcaa
     2761 cggcattagg gataaacagt caggaaagac aatcctggac tttctgaaaa gtgatggctt
     2821 cgccaaccgc aattttatgc agctgattca cgatgacagt ctgaccttca aagaggacat
     2881 ccagaaggct caggtgtctg gacagggcca ctccctgcat gagcagattg caaacctggc
     2941 tggaagccca gccatcaaga aaggcattct gcagacagtg aaaatcgtcg atgagctggt
     3001 gaaagtcatg ggccataagc ccgaaaacat cgtgattgag atggctcgcg aaaatcagac
     3061 aacccagaag ggtcagaaga acagtagaga gaggatgaaa agaatcgagg aaggcattaa
     3121 ggagctgggt agccagatcc tgaaagagca cccagtggaa aacacacagc tgcagaatga
     3181 gaagctgtat ctgtactatc tgcagaatgg aagagatatg tacgtggacc aggagctgga
     3241 tattaacagg ctgtctgatt acgacgtgga tcatatcgtc ccccagagtt tcatcaaaga
     3301 tgacagcatt gacaacaagg tgctgaccag gtccgacaaa aacagaggaa aatcagataa
     3361 tgtccctagt gaggaagtgg tcaagaaaat gaagaactac tggagacagc tgctgaatgc
     3421 caaactgatc actcagagga agtttgataa cctgacaaaa gcagagcgcg gtggactgtc
     3481 agaactggac aaagctggat tcatcaagag gcagctggtg gaaacacgcc agatcactaa
     3541 acacgtcgca cagattctgg atagtcgcat gaacacaaag tacgatgaga atgacaaact
     3601 gatcagagaa gtgaaggtca ttaccctgaa gagtaaactg gtcagcgact ttaggaaaga
     3661 tttccagttt tataaggtcc gcgagattaa caattatcac catgcccatg acgcatacct
     3721 gaacgccgtg gtcggtaccg cactgatcaa gaaataccca aaactggaga gcgaattcgt
     3781 gtacggagac tataaggtgt acgatgtcag aaaaatgatc gccaagtccg agcaggaaat
     3841 tggaaaagct actgccaagt atttctttta ctcaaacatc atgaatttct ttaagacaga
     3901 gatcaccctg gccaatggag aaatccgcaa aaggcccctg attgagacaa acggagagac
     3961 aggcgaaatc gtgtgggaca aaggcagaga ttttgcaacc gtgaggaagg tcctgagcat
     4021 gcctcaagtg aatatcgtca agaaaactga ggtgcagaca ggcggtttct caaaagaaag
     4081 tattctgcca aaacgcaact ctgataagct gatcgctaga aagaaagact gggaccctaa
     4141 gaagtatgga ggctttgact ctcccactgt ggcatactcc gtcctggtgg tcgctaaggt
     4201 ggagaagggc aaaagcaaga aactgaaatc tgtcaaggag ctgctgggta tcacaattat
     4261 ggagagaagc tctttcgaga agaacccaat cgattttctg gaggccaaag gttataagga
     4321 agtgaagaaa gacctgatca ttaaactgcc caagtacagt ctgtttgagc tggaaaacgg
     4381 caggaaacgc atgctggcaa gcgctggaga gctgcagaaa ggcaatgaac tggccctgcc
     4441 ttctaagtac gtgaacttcc tgtatctggc aagccactac gagaagctga aaggatctcc
     4501 agaggataac gaacagaaac agctgtttgt ggagcagcac aagcattatc tggacgagat
     4561 cattgaacag attagcgagt tctctaaaag agtgatcctg gccgacgcaa atctggataa
     4621 ggtcctgtct gcttacaaca aacacagaga taagcccatc agggagcagg ccgaaaatat
     4681 cattcatctg ttcactctga caaacctggg cgcacctgca gctttcaagt acttcgacac
     4741 tacaatcgat agaaagaggt acacctccac taaggaggtg ctggacgcta cactgatcca
     4801 tcagagtatt accggcctgt acgaaacaag gattgacctg tctcagctgg gtggcgactc
     4861 accggtggga tcccctaaga agaagagaaa ggtgggatcc ggagccacga acttctctct
     4921 gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct atggtgagca agggcgagga
     4981 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     5041 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5101 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     5161 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     5221 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5281 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     5341 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     5401 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     5461 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     5521 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     5581 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     5641 caccggcggc atggacgagc tgtacaagta aggatccact agttctagag cggccgcggt
     5701 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     5761 catagcaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     5821 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     5881 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     5941 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     6001 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     6061 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     6121 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     6181 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     6241 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     6301 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     6361 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     6421 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     6481 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     6541 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     6601 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     6661 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     6721 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     6781 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     6841 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     6901 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     6961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7621 gctggccttt tgctcacatg tt
//
