LOCUS       sequence-212864-        7895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of CD44 variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7895)
  TITLE     Georg Weber Lab Plasmids
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe-CD44v8
FEATURES             Location/Qualifiers
     source          1..7895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    2..569
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    19..571
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(163..180)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     390..409
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(583..585,586..588,589..1302)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(583..585,586..588,589..1299)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(628..649)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(889..908)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1236..1257
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1488..1957
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2087..2104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2258..2846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2338..2357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2970..3038,3039..3830)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3188..3207)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     LTR             4512..4981
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5046..5403
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5468..5884
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5814..5836
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5852..5868
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5894..6268
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     6206..6222
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
       61 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
      121 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
      181 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
      241 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
      301 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
      361 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
      421 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
      481 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
      541 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
      601 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      661 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      721 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      781 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      841 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      901 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      961 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1021 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1081 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1141 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1201 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1261 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcgccag cacagtggtc
     1321 gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     1381 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     1441 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     1501 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     1561 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     1621 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     1681 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     1741 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     1801 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     1861 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     1921 tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa
     1981 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
     2041 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2101 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2161 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2221 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2341 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2401 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2461 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2521 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2581 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2641 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2701 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2761 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2821 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2881 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagacaataa ccctgataaa
     2941 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3001 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3061 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3121 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3181 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3241 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3301 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3361 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3421 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3481 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3541 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3601 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3661 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3721 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3781 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3841 aagtttactc atatatactt tagattgatt tgcggccggc cgcaaaagga tctaggtgaa
     3901 gatccttttt gataatctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3961 aataggggtt ccgcgcacat ttccccgaaa agtgccacca gctttgctct taggagtttc
     4021 ctaatacatc ccaaactcaa atatataaag catttgactt gttctatgcc ctagggggcg
     4081 gggggaagct aagccagctt tttttaacat ttaaaatgtt aattccattt taaatgcaca
     4141 gatgttttta tttcataagg gtttcaatgt gcatgaatgc tgcaatattc ctgttaccaa
     4201 agctagtata aataaaaata gataaacgtg gaaattactt agagtttctg tcattaacgt
     4261 ttccttcctc agttgacaac ataaatgcgc tgctgagaag ccagtttgca tctgtcagga
     4321 tcaatttccc attatgccag tcatattaat tactagtcaa ttagttgatt tttatttttg
     4381 acatatacat gtgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     4441 ttgcaaggca tggaaaaata cataactgag aatagaaaag ttcagatcaa ggtcaggaac
     4501 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     4561 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     4621 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca
     4681 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     4741 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     4801 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgattgactg
     4861 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     4921 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     4981 atttgggggc tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg
     5041 gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat
     5101 tttatgcgcc tgcgtcggta ctagttagct aactagctct gtatctggcg gacccgtggt
     5161 ggaactgacg agttcggaac acccggccgc aaccctggga gacgtcccag ggacttcggg
     5221 ggccgttttt gtggcccgac ctgagtccaa aaatcccgat cgttttggac tctttggtgc
     5281 acccccctta gaggagggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     5341 gcctccgtct gaatttttgc tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg
     5401 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat
     5461 gggcccgggc cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt
     5521 cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg
     5581 ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga
     5641 cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca
     5701 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
     5761 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
     5821 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
     5881 cgcccccata tggccatatg agatcttata tggggcaccc ccgccccttg taaacttccc
     5941 tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact tacaggctct
     6001 ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag aacaactgga
     6061 ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca
     6121 gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac
     6181 cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga
     6241 ccccgggggt ggaccatcct ctagactgcc ggatccattt aaattcgaat tcctgcaggc
     6301 ctcgagggcc ggcgcgccgc ggtggcggcc gctctagccc ctcgctcgcc cacgccatgg
     6361 acaagttttg gtggcacaca gcttggggac tttgcctctt gcagttgagc ctggcacatc
     6421 agcagatcga tttgaatgta acctgccgct acgcaggtgc attccatgtg gagaaaaatg
     6481 gccgctacag tatctcccgg actgaggcag ctgacctctg ccaggctttc aacagtacct
     6541 tacccaccat ggaccaaatg aagttggccc tgagcaaggg ttttgagaca tgcaggtatg
     6601 ggttcataga aggaaatgtg gtaattccga ggattcatcc caacgctatc tgtgcagcca
     6661 accacacagg agtatatatc ctcgtcacgt ccaacacctc ccactatgac acatattgct
     6721 tcaatgcctc agcccctcct gaagaagact gtacatcagt cacagaccta cccaattcct
     6781 tcgatggacc cgttaccata actattgtca accgtgatgg tacacgctac agcaagaagg
     6841 gcgagtatag aacacaccaa gaagacatcg atgcttcaaa cattatagat gacgatgtca
     6901 gcagcggctc caccatcgag aagagcaccc cagaaggcta cattttgcac acctaccttc
     6961 ctactgaaca gcctactgga gatcaggatg actccttctt tatccggagc accttggcca
     7021 ccaatacaga ctccagtcat aatacaaccc ttcagcctac tgcagctcca aatacccatt
     7081 tggtggaaga cttgaacagg acaggaccac tttcagtgac aactccacag agtcattctc
     7141 agaacttctc tacattacat ggagagccgg aagaagacga aaactatcca acaacttcta
     7201 ttctgccatc tagcactaag agcagcgcta aagatgcaag aagaggtgga agtcttccca
     7261 cagatacaac tacttctgta gaaggctaca cctttcaata cccagacaca atggaaaatg
     7321 ggactctctt cccagtgacc cctgctaaaa ctgaggtctt tggagaaact gaagtgactc
     7381 ttgctactga ctccaacgtt aatgttgatg gctccttacc aggagatcga gactcatcca
     7441 aggactccag ggggagttcc cgcactgtga ctcatggatc cgaattagct ggacactcaa
     7501 gtgcgaacca ggacagtgga gtgaccacaa cttctggtcc tatgaggaga cctcagattc
     7561 cagaatggct catcatcttg gcatctctcc tggcactggc tctgattctt gccgtctgca
     7621 tcgcggtcaa tagtaggaga aggtgtgggc agaagaaaaa gctggtgatc aacggtggca
     7681 atgggacagt ggaagacagg aaacccagtg agctcaacgg ggaggccagc aagtctcagg
     7741 aaatggtgca tttggtgaac aaggaaccat cagagacccc agaccagtgt atgacagctg
     7801 acgagacccg gaatctgcag agtgtggaca taaatggggt gtagtgcctg ggccgaacca
     7861 ggggcggatc ccccgtaaat tccgcccccc ccccc
//
