LOCUS       sequence-212867-        8027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8027)
  TITLE     Georg Weber Lab Plasmids
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe-CD44v7
FEATURES             Location/Qualifiers
     source          1..8027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    257..614
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             679..1095
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1025..1047
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1063..1079
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1105..1479
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1417..1433
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(3234..3808,3809..3811,3812..3820)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3401..3418)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3628..3647
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3821..3823,3824..3826,3827..4540)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3821..3823,3824..3826,3827..4537)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3866..3887)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4127..4146)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4474..4495
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4726..5195
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5325..5342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5496..6084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5576..5595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6208..6276,6277..7068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6426..6445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     LTR             join(7750..8027,1..192)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
       61 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
      121 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
      181 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
      241 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      301 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc
      361 ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca
      421 gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga
      481 ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta
      541 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg
      601 cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
      661 tctgaaaata tgggcccggg ccagactgtt accactccct taagtttgac cttaggtcac
      721 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
      781 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
      841 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
      901 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
      961 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
     1021 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
     1081 ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt
     1141 gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac
     1201 ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc agcctaccaa
     1261 gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac agtgtgggtc
     1321 cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac agtcctgctg
     1381 accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc cgcccacgtg
     1441 aaggctgccg accccggggg tggaccatcc tctagactgc cggatccatt taaattcgaa
     1501 ttcctgcagg cctcgagggc cggcgcgccg cggtggcggc cgctctagcc cctcgctcgc
     1561 ccacgccatg gacaagtttt ggtggcacac agcttgggga ctttgcctct tgcagttgag
     1621 cctggcacat cagcagatcg atttgaatgt aacctgccgc tacgcaggtg tattccatgt
     1681 ggagaaaaat ggccgctaca gtatctcccg gactgaggca gctgacctct gccaggcttt
     1741 caacagtacc ttacccacca tggaccaaat gaagttggcc ctgagcaagg gttttgaaac
     1801 atgcaggtat gggttcatag aaggaaatgt ggtaattccg aggattcatc ccaacgctat
     1861 ctgtgcagcc aaccacacag gagtatatat cctcgtcacg tccaacacct cccactatga
     1921 cacatattgc ttcaatgcct cagcccctcc tgaagaagac tgtacatcag tcacagacct
     1981 acccaattcc ttcgatggac ccgttaccat aactattgtc aaccgtgatg gtacacgcta
     2041 cagcaagaag ggcgagtata gaacacacca agaagacatc gatgcttcaa acattataga
     2101 tgacgatgtc agcagcggct ccaccatcga gaagagcacc ccagaaggct acattttgca
     2161 cacctacctt cctactgaac agcctactgg agatcaggat gactccttct ttatccggag
     2221 caccttggcc accacttcgg cccacaacaa ccatccaagt caaagaataa caacacagag
     2281 tcaagaggat gtttcctgga cagatttctt cgacccaatc tcacatccaa tgggacaagg
     2341 tcatcaaaca gaaagcaagg atacagactc cagtcatagt acaacccttc agcctactgc
     2401 agctccaaat acccatttgg tggaagactt gaacaggaca ggaccacttt cagtgacaac
     2461 tccacagagt cattctcaga acttctctac attacatgga gagccggaag aagacgaaaa
     2521 ctatccaaca acttctattc tgccatctag cactaagagc agcgctaaag atgcaagaag
     2581 aggtggaagt cttcccacag atacaactac ttctgtagaa ggctacacct ttcaataccc
     2641 agacacaatg gaaaatggga ctctcttccc agtgacccct gctaaaactg aggtctttgg
     2701 agaaactgaa gtgactcttg ctactgactc caacgttaat gttgatggct ccttaccagg
     2761 agatcgagac tcatccaagg actccagggg gagttcccgc actgtgactc atggatccga
     2821 attagctgga cactcaagtg cgaaccagga cagtggagtg accacaactt ctggtcctat
     2881 gaggagacct cagattccag aatggctcat catcttggca tctctcctgg cactggctct
     2941 gattcttgcc gtctgcatcg cggtcaatag taggagaagg tgtgggcaga agaaaaagct
     3001 ggtgatcaac ggtggcaatg ggacagtgga agacaggaaa cccagtgagc tcaacgggga
     3061 ggccagcaag tctcaggaaa tggtgcattt ggtgaacaag gaaccatcag agaccccaga
     3121 ccagtgtatg acagctgacg agacccggaa tctgcagagt gtggacatga agattggggt
     3181 gtagtgcctg ggccgaacca ggggcggatc ccccgtaaat tccgcccccc ccccccctct
     3241 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     3301 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     3361 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     3421 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     3481 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     3541 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     3601 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     3661 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     3721 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     3781 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
     3841 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3901 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3961 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4021 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4081 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4141 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4201 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4261 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4321 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4381 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4441 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4501 cgccgggatc actctcggca tggacgagct gtacaagtaa agcgccagca cagtggtcga
     4561 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     4621 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     4681 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     4741 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     4801 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     4861 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     4921 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     4981 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     5041 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     5101 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     5161 tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt
     5221 tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa
     5281 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5881 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6121 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gacaataacc ctgataaatg
     6181 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6241 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6301 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6361 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6421 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6481 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6541 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6601 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6661 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6721 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6781 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6841 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6901 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6961 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7021 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7081 gtttactcat atatacttta gattgatttg cggccggccg caaaaggatc taggtgaaga
     7141 tcctttttga taatctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7201 taggggttcc gcgcacattt ccccgaaaag tgccaccagc tttgctctta ggagtttcct
     7261 aatacatccc aaactcaaat atataaagca tttgacttgt tctatgccct agggggcggg
     7321 gggaagctaa gccagctttt tttaacattt aaaatgttaa ttccatttta aatgcacaga
     7381 tgtttttatt tcataagggt ttcaatgtgc atgaatgctg caatattcct gttaccaaag
     7441 ctagtataaa taaaaataga taaacgtgga aattacttag agtttctgtc attaacgttt
     7501 ccttcctcag ttgacaacat aaatgcgctg ctgagaagcc agtttgcatc tgtcaggatc
     7561 aatttcccat tatgccagtc atattaatta ctagtcaatt agttgatttt tatttttgac
     7621 atatacatgt gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     7681 gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag
     7741 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     7801 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     7861 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     7921 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     7981 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgc
//
