LOCUS       sequence-212868-       12840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, 
            Varjosalo M
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping
            of protein interactions and subcellular localizations.
  JOURNAL   Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2.
  PUBMED    29568061
REFERENCE   2  (bases 1 to 12840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-22"; volume: "9(1):1188. doi"; pages: " 
            10.1038/s41467-018-03523-2"
COMMENT     Sequence Label: MAC_N_MED13
FEATURES             Location/Qualifiers
     source          1..12840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        24..403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        404..607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     558..578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    609..627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    630..648
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     658..682
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(850..1200,1201..1203,1204..1812)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1813..1839
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1870..1893
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1930..1953
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1966..1990
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3077..3086
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8519..8543)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8581..8598)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8587..8811
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8944..8963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9095..9142
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9150..10172
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9150..10172
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10302..10423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10339..10358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10393..10412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10472..10488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10472..10488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10485..10507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10496..10512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10520..10526,10527..10544,10545..10550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10565..10586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10703..10720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10874..11462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10954..10973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11633..12424,12425..12493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12256..12275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12494..12598)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12673..12692)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
       61 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      121 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      181 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      241 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      301 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      361 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      421 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      481 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      541 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      601 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
      661 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      721 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga
      781 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
      841 ctcgagacca tgaaggacaa caccgtgccc ctgaagctga tcgccctgct ggccaacggc
      901 gagttccact ctggcgagca gctgggcgag acactgggca tgagcagagc cgccatcaac
      961 aagcacatcc agaccctgcg ggactggggc gtggacgtgt tcacagtgcc cggcaagggc
     1021 tacagcctgc ccgagcccat ccagctgctg aacgccaagc agatcctggg ccagctggat
     1081 ggcggatctg tggccgtgct gcccgtgatc gacagcacca accagtatct gctcgaccgg
     1141 atcggcgagc tgaagtccgg cgacgcctgt atcgccgagt accagcaggc cggcagaggc
     1201 ggcaggggca gaaagtggtt cagccccttc ggcgccaacc tgtacctgag catgttctgg
     1261 agactggaac agggcccagc cgccgctatt ggcctgagcc tggtcatcgg catcgtgatg
     1321 gccgaggtgc tgcggaagct gggcgccgac aaagtgcgcg tgaagtggcc caacgacctg
     1381 tatctgcagg accggaagct ggccggcatc ctggtggaac tgaccggcaa aacaggcgac
     1441 gccgcccaga tcgtgatcgg cgctggcatc aacatggcca tgcggcgggt ggaagagagc
     1501 gtggtcaacc agggctggat caccctgcag gaagccggca tcaacctgga ccggaacacc
     1561 ctggccgcca tgctgatcag agagctgaga gccgccctgg aactgttcga gcaggaaggc
     1621 ctggccccct acctgagcag atgggagaag ctggacaact tcatcaaccg gcccgtgaaa
     1681 ctgatcatcg gcgacaaaga gatcttcggc atcagccggg gcatcgacaa gcagggggcc
     1741 ctgctcctgg aacaggacgg catcatcaag ccctggatgg gcggcgagat cagcctgcgg
     1801 agcgccgaga agtacccata cgatgttcct gactatgccg gtaccgagct cggatccacc
     1861 atggctagct ggagccaccc gcagttcgag aaaggtggag gttccggagg tggatcggga
     1921 ggtggatcgt ggagccaccc gcagttcgaa aaagcggccg atatcacaag tttgtacaaa
     1981 aaagcaggct tcaccatgag tgcctccttc gtgccgaacg gggccagcct ggaagattgt
     2041 cactgtaacc tcttctgcct ggctgacttg acaggaatta agtggaaaaa atatgtatgg
     2101 caaggcccaa cttctgcccc tattctgttt cctgtgacag aagaagaccc cattttgagc
     2161 agttttagtc gctgccttaa ggcagatgta cttggtgttt ggcggcgaga tcaaagacct
     2221 ggaagaagag aattgtggat attttggtgg ggtgaagacc ccagttttgc tgaccttatt
     2281 caccatgact tatcagaaga agaagatgga gtgtgggaga atggactttc ctatgaatgc
     2341 cgtactctgc ttttcaaagc agttcacaat ctattggaac ggtgtttaat gaacaggaat
     2401 tttgtacgta ttggcaagtg gtttgtaaag ccttatgaaa aagatgaaaa acctataaat
     2461 aaaagtgaac acttgtcctg ctccttcacc tttttcttgc atggagacag caatgtttgt
     2521 accagtgtgg aaattaacca acatcaacct gtataccttc tcagtgaaga gcatatcacc
     2581 cttgctcaac agtctaatag cccatttcaa gttatcttat gcccatttgg actaaatggc
     2641 actctcacag gacaggcatt caagatgtct gattcagcta caaaaaaatt aattggtgaa
     2701 tggaaacagt tctatcctat ctcatgttgc ttgaaggaga tgtctgaaga aaaacaggaa
     2761 gatatggatt gggaagatga ttctttagct gcagtagaag ttcttgttgc tggtgtccga
     2821 atgatctacc cagcatgctt tgttctagtc cctcagtcag acattcctac tcctagccct
     2881 gtgggatcca ctcactgttc atcttcttgc ttgggtgtcc accaagtgcc tgcttccaca
     2941 agagatcctg ctatgtcttc ggttacgctt acaccaccta cgtctcctga ggaagtccaa
     3001 acagttgatc ctcagtctgt ccagaagtgg gtcaaatttt cttcagtatc tgatggcttc
     3061 aactccgata gtactagcca ccatggtggg aaaataccca gaaaattagc aaatcatgtg
     3121 gtggatagag tttggcaaga atgcaatatg aacagagcac agaacaagag gaagtattct
     3181 gcttcatcag gtggtctatg cgaagaagcg acagctgcta aagtggcatc ctgggatttt
     3241 gttgaagcca cacaaagaac aaattgcagt tgtttgaggc acaaaaatct caagtcaaga
     3301 aatgctggac aacaaggaca ggcaccatct ttaggtcagc aacaacaaat acttcctaag
     3361 cacaagacca atgagaagca agaaaagagt gaaaagccac agaaacgccc cttgactcct
     3421 tttcaccatc gtgtgtctgt tagtgatgat gttggcatgg acgcagattc agccagccaa
     3481 agacttgtga tctctgctcc agacagtcaa gtgagatttt caaatatccg aactaatgat
     3541 gtagcaaaga ctcctcagat gcatggcacc gaaatggcaa attcacctca accaccccca
     3601 cttagtcctc acccttgtga tgtggttgat gaaggagtga ctaaaacacc ttcaactcct
     3661 cagagtcaac atttttatca aatgccaaca ccagatccct tggttccttc taaaccaatg
     3721 gaagatagga tagacagttt gtcccagtct ttcccacctc aatatcagga agctgtagaa
     3781 cctacagtat atgttggtac agcagtaaac ttggaagaag atgaagccaa tatagcctgg
     3841 aagtattaca agttcccaaa gaaaaaagat gtagagtttt taccacctca acttccaagt
     3901 gataaattca aggatgatcc agttggacct tttggacagg aaagtgtaac atcagttaca
     3961 gagttaatgg tgcaatgtaa gaaaccttta aaagtttctg atgaattagt gcagcaatat
     4021 caaattaaaa accagtgtct ttcagcaata gcatctgatg cagaacaaga acctaaaatt
     4081 gatccatatg catttgttga aggagatgag gaattccttt ttcctgataa aaaagataga
     4141 caaaatagtg agagagaagc tggaaaaaaa cacaaggtag aagatgggac atctagtgta
     4201 acagtgttat cacatgaaga agatgctatg tcattattta gtccctctat caagcaagat
     4261 gctccacgcc ctactagtca tgcccgtcct ccatcaacaa gtttgattta tgactcagac
     4321 ctggctgtct cttatactga ccttgataat ctcttcaatt ctgatgaaga tgaactaaca
     4381 cctggatcta aaaaatcagc aaatggatca gatgataaag ccagctgcaa ggaatcaaag
     4441 acaggaaatc tggacccgtt atcttgcata agcactgcag atcttcataa aatgtatcct
     4501 acaccaccat cattggaaca acatattatg ggattttccc caatgaatat gaataataaa
     4561 gaatatggta gtatggatac aacacctgga ggaactgttc tagaaggaaa tagttctagt
     4621 ataggagcgc agttcaaaat tgaggttgat gagggattct gtagccccaa accttctgaa
     4681 attaaagatt tttcttatgt ctataagcct gaaaattgtc aaattctagt gggatgttcc
     4741 atgtttgcac ctctaaaaac tctaccaagc caatatctgc cccctatcaa attgccagaa
     4801 gagtgtattt accgtcagag ttggactgtt ggaaaattgg aattgctttc ttcagggcct
     4861 tcaatgccat tcatcaaaga gggtgatgga agtaatatgg atcaagaata tggcactgct
     4921 tatacacctc aaactcatac ttcttttggg atgcctccta gcagtgcacc tcctagtaac
     4981 agcggagcag gaattcttcc ttctccatcc acccctcggt ttccaactcc aaggactcca
     5041 aggactcctc ggactcctcg tggagctggt ggacctgcta gtgctcaagg ttcagtcaaa
     5101 tatgaaaatt cagacttgta ttcaccagct tctaccccat ctacatgcag accccttaat
     5161 tctgttgaac ctgcaactgt cccttccatc cctgaagcac acagtcttta tgtaaacctc
     5221 atcctttcag aatcagttat gaatttgttt aaagactgta actttgatag ttgttgcatc
     5281 tgtgtttgca acatgaacat caagggtgcc gatgttggag tttacattcc agatccaacg
     5341 caggaagcac aatataggtg tacctgtggc ttcagtgctg tcatgaacag aaaatttgga
     5401 aacaattcag gattatttct tgaagatgaa ctagatatca taggacgcaa tacagactgt
     5461 ggcaaagaag cagaaaaacg ttttgaagct ctcagggcta cctctgctga acatgttaat
     5521 ggaggactaa aggaatctga aaaattatct gatgatttga tattattgct acaagatcag
     5581 tgcactaatt tattttcacc ctttggagca gcagaccaag atccttttcc taaaagtggt
     5641 gtaattagca attgggtacg tgttgaagag cgtgactgtt gcaatgactg ctaccttgca
     5701 ttagaacatg ggcgtcagtt catggataac atgtcaggag gaaaagttga tgaagcactt
     5761 gtgaaaagtt catgcttaca cccctggtcc aaaagaaacg atgtgagtat gcagtgctca
     5821 caggatatac ttcgaatgct cctctctctt cagccagttc ttcaggatgc cattcagaaa
     5881 aaaagaacag taagaccttg gggtgttcag ggtcctctca cttggcaaca atttcataaa
     5941 atggctggcc gaggctctta tggaactgat gaatccccag aaccactgcc aatccccaca
     6001 tttttgttgg gttatgatta tgattatctg gtgctttctc catttgctct tccttattgg
     6061 gagagactta tgctggaacc ctatggatct caaagagata tagcctatgt tgtactgtgt
     6121 ccagaaaatg aagccttgtt aaatggagca aaaagctttt ttagagatct tactgcaata
     6181 tatgagtcct gtcgattagg tcaacataga cctgtttctc gactgttaac agatgggatc
     6241 atgagagttg gatctactgc atcaaagaaa ctatcagaaa agttggtagc agaatggttt
     6301 tctcaggcag ctgacggtaa caatgaagca ttttctaaac tcaagcttta tgcacaagtc
     6361 tgcagatatg acctaggtcc ttatcttgct tccctgccat tggacagctc tctactttcc
     6421 cagccaaatt tagttgcccc tacaagtcag tctttgatta ctccacctca gatgacaaat
     6481 actggaaatg ctaatactcc atctgccacc ttagcatctg cagcgagcag cactatgaca
     6541 gtgacttcag gtgttgccat atctacttca gttgccacag ctaattcaac tttgaccaca
     6601 gcttcaactt catcttcatc atcctccaac ttgaatagtg gagtatcatc aaataaacta
     6661 ccttcgtttc caccctttgg cagtatgaac agtaatgctg caggatccat gtctacacaa
     6721 gcaaatacag ttcagagtgg tcagctagga gggcaacaga catcagctct acagacagct
     6781 gggatttctg gagaatcatc ttcacttccc actcagccgc atcctgatgt gtctgaaagc
     6841 acgatggatc gggataaagt gggaatcccc acagatggtg attcacatgc agtcacgtat
     6901 ccacctgcaa ttgttgttta tataattgat ccttttacat acgaaaatac agacgagagc
     6961 actaactctt ctagtgtgtg gacattgggg ctacttcgat gctttctaga aatggtccag
     7021 actcttcctc ctcatatcaa gagtactgtt tctgtacaga ttattccttg tcagtacctg
     7081 ttgcaacctg tgaagcatga agatagagaa atctatcccc agcatttaaa atccctggct
     7141 ttttcggcct ttacccagtg tcggaggcca cttccaacat caaccaatgt gaaaacattg
     7201 actggctttg gtccaggttt agccatggaa actgccctta gaagtcctga tagaccagag
     7261 tgtattcgac tttatgcacc tccttttatt ctggctccag tgaaggacaa acagacagag
     7321 ctaggagaaa catttggaga agctggacag aaatataatg ttctttttgt gggatactgt
     7381 ttatcacatg atcaaaggtg gattcttgca tcttgcacag atctatatgg agaactttta
     7441 gaaacttgta tcattaacat cgatgttcca aatagggctc gtcggaaaaa aagttctgct
     7501 agaaaatttg gtctacagaa actttgggag tggtgcttag gacttgtaca aatgagttca
     7561 ttgccatgga gagttgtaat tggtcgtcta ggaaggattg gtcatggaga attgaaagat
     7621 tggagctgtt tgctgagtcg tcgaaacttg cagtctctaa gtaaaaggct caaagacatg
     7681 tgtagaatgt gtggtatatc tgctgcagac tcccctagca ttctcagtgc ttgcttggtg
     7741 gcaatggagc cgcaaggctc ttttgttatt atgccagatt ctgtgtcaac tggttctgta
     7801 tttggaagaa gcacgactct aaatatgcag acatctcagc taaatacccc acaggataca
     7861 tcatgtactc atatacttgt gtttcctact tctgcttctg tgcaagtagc ttcagctact
     7921 tataccactg aaaatttgga tttagctttc aatcccaaca atgatggagc agatggaatg
     7981 ggtatctttg atttgttaga cacaggagat gatcttgacc ctgatatcat taatatcctt
     8041 cctgcttctc caactggttc tcctgtacat tctccaggat ctcattaccc ccatggaggt
     8101 gatgcgggca agggtcagag tactgatcgg ctactatcaa cagaacctca tgaggaagta
     8161 cctaatattc ttcagcaacc attggccctt ggttactttg tatcaactgc caaagcaggt
     8221 ccattacctg actggttctg gtcagcatgt cctcaagcac aatatcagtg tccccttttt
     8281 cttaaggcct ctttgcacct ccacgtgcct tcagtgcaat ctgacgagct gcttcacagt
     8341 aaacactccc acccacttga ctcaaatcag acttcagatg tcctcaggtt tgttttggaa
     8401 cagtacaatg cactctcctg gctaacctgt gaccctgcaa cccaggacag acgctcatgt
     8461 ctcccaattc attttgtggt gctgaatcag ttatataact ttattatgaa tatgctggac
     8521 ccagctttct tgtacaaagt ggtgacgtaa gctaggggcc cgtttaaacc cgctgatcag
     8581 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8641 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8701 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8761 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8821 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     8881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9001 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9061 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     9121 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     9181 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     9241 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     9301 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     9361 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     9421 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     9481 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     9541 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     9601 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     9661 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     9721 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     9781 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     9841 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     9901 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     9961 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
    10021 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
    10081 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
    10141 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
    10201 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10261 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    10441 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10501 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10561 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    10621 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    10681 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    10741 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    10801 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11281 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    11341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11461 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11521 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11581 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11641 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11761 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11821 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11881 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11941 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12001 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12061 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12121 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12181 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12241 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12301 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12361 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12421 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12481 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12601 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    12661 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    12721 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    12781 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
//
