LOCUS       sequence-212873-       16768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are 
            expressed as a single poly-cistronic unit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
            Tong J, Cheng L
  TITLE     Efficient human iPS cell derivation by a non-integrating plasmid 
            from blood cells with unique epigenetic and gene expression 
            signatures.
  JOURNAL   Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 
            Jan 18.
  PUBMED    21243013
REFERENCE   2  (bases 1 to 16768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2011.12"; journalName: "Cell Res"; date: "2011-03"; 
            volume: "21"; issue: "3"; pages: "518-29"
COMMENT     Sequence Label: pEB-C5
FEATURES             Location/Qualifiers
     source          1..16768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     enhancer        570..949
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        951..1226
                     /label=chicken beta-actin promoter
     intron          1227..2243
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2167..2189
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2251..2270
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3381..3434
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4410..4463
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7299..7352
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    8002..8136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8026..8045)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8080..8099
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8279..8301)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12483..12501)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12569..12673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12674..12742,12743..13534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12892..12911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13705..14293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14194..14213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        14724..14869
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             14912..15949
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(15947..15966)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15991..16039
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        join(16693..16768,1..304)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct ctagaagctg
      541 ggtaccagct gctagcatgg gcccatctcg acattgatta ttgactagtt attaatagta
      601 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      661 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      721 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      781 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      841 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      901 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
      961 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     1021 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgccag
     1081 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     1141 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     1201 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
     1261 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     1321 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     1381 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     1441 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     1501 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     1561 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     1621 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     1681 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     1741 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     1801 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc tcgggggagg
     1861 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     1921 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     1981 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     2041 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2101 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     2161 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     2221 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     2281 attttggcaa agaattcaga tccaccatgg ctggacacct ggcttcagac ttcgccttct
     2341 cacccccacc aggtgggggt gatgggtcag cagggctgga gccgggctgg gtggattctc
     2401 gaacctggct aagcttccaa gggcctccag gtgggcctgg aatcggacca ggctcagagg
     2461 tattggggat ctccccatgt ccgcccgcat acgagttctg cggagggatg gcatactgtg
     2521 gacctcaggt tggactgggc ctagtccccc aagttggcgt ggagactttg cagcctgagg
     2581 gccaggcagg agcacgagtg gaaagcaact cagagggaac ctcctctgag ccctgtgccg
     2641 accgccccaa tgccgtgaag ttggagaagg tggaaccaac tcccgaggag tcccaggaca
     2701 tgaaagccct gcagaaggag ctagaacagt ttgccaagct gctgaagcag aagaggatca
     2761 ccttggggta cacccaggcc gacgtggggc tcaccctggg cgttctcttt ggaaaggtgt
     2821 tcagccagac caccatctgt cgcttcgagg ccttgcagct cagccttaag aacatgtgta
     2881 agctgcggcc cctgctggag aagtgggtgg aggaagccga caacaatgag aaccttcagg
     2941 agatatgcaa atcggagacc ctggtgcagg cccggaagag aaagcgaact agcattgaga
     3001 accgtgtgag gtggagtctg gagaccatgt ttctgaagtg cccgaagccc tccctacagc
     3061 agatcactca catcgccaat cagcttgggc tagagaagga tgtggttcga gtatggttct
     3121 gtaaccggcg ccagaagggc aaaagatcaa gtattgagta ttcccaacga gaagagtatg
     3181 aggctacagg gacacctttc ccaggggggg ctgtatcctt tcctctgccc ccaggtcccc
     3241 actttggcac cccaggctat ggaagccccc acttcaccac actctactca gtcccttttc
     3301 ctgagggcga ggcctttccc tctgttcccg tcactgctct gggctctccc atgcattcaa
     3361 acggcggcgg gtccggagga gagggcagag gaagtcttct aacatgcggt gacgtggagg
     3421 agaatcctgg cccaatgtat aacatgatgg agacggagct gaagccgccg ggcccgcagc
     3481 aagcttcggg gggcggcggc ggaggaggca acgccacggc ggcggcgacc ggcggcaacc
     3541 agaagaacag cccggaccgc gtcaagaggc ccatgaacgc cttcatggta tggtcccggg
     3601 ggcagcggcg taagatggcc caggagaacc ccaagatgca caactcggag atcagcaagc
     3661 gcctgggcgc ggagtggaaa cttttgtccg agaccgagaa gcggccgttc atcgacgagg
     3721 ccaagcggct gcgcgctctg cacatgaagg agcacccgga ttataaatac cggccgcggc
     3781 ggaaaaccaa gacgctcatg aagaaggata agtacacgct tcccggaggc ttgctggccc
     3841 ccggcgggaa cagcatggcg agcggggttg gggtgggcgc cggcctgggt gcgggcgtga
     3901 accagcgcat ggacagctac gcgcacatga acggctggag caacggcagc tacagcatga
     3961 tgcaggagca gctgggctac ccgcagcacc cgggcctcaa cgctcacggc gcggcacaga
     4021 tgcaaccgat gcaccgctac gacgtcagcg ccctgcagta caactccatg accagctcgc
     4081 agacctacat gaacggctcg cccacctaca gcatgtccta ctcgcagcag ggcacccccg
     4141 gtatggcgct gggctccatg ggctctgtgg tcaagtccga ggccagctcc agcccccccg
     4201 tggttacctc ttcctcccac tccagggcgc cctgccaggc cggggacctc cgggacatga
     4261 tcagcatgta cctccccggc gccgaggtgc cggagcccgc tgcgcccagt agactgcaca
     4321 tggcccagca ctaccagagc ggcccggtgc ccggcacggc cattaacggc acactgcccc
     4381 tgtcgcacat gggcggcggg tccggaggag agggcagagg aagtcttcta acatgcggtg
     4441 acgtggagga gaatcctggc ccaatggctg tcagcgacgc tctgctcccg tccttctcca
     4501 cgttcgcgtc cggcccggcg ggaagggaga agacactgcg tccagcaggt gccccgacta
     4561 accgttggcg tgaggaactc tctcacatga agcgacttcc cccacttccc ggccgcccct
     4621 acgacctggc ggcgacggtg gccacagacc tggagagtgg cggagctggt gcagcttgca
     4681 gcagtaacaa cccggccctc ctagcccgga gggagaccga ggagttcaac gacctcctgg
     4741 acctagactt tatcctttcc aactcgctaa cccaccagga atcggtggcc gccaccgtga
     4801 ccacctcggc gtcagcttca tcctcgtctt ccccagcgag cagcggccct gccagcgcgc
     4861 cctccacctg cagcttcagc tatccgatcc gggccggggg tgacccgggc gtggctgcca
     4921 gcaacacagg tggagggctc ctctacagcc gagaatctgc gccacctccc acggccccct
     4981 tcaacctggc ggacatcaat gacgtgagcc cctcgggcgg cttcgtggct gagctcctgc
     5041 ggccggagtt ggacccagta tacattccgc cacagcagcc tcagccgcca ggtggcgggc
     5101 tgatgggcaa gtttgtgctg aaggcgtctc tgaccacccc tggcagcgag tacagcagcc
     5161 cttcggtcat cagtgttagc aaaggaagcc cagacggcag ccaccccgtg gtagtggcgc
     5221 cctacagcgg tggcccgccg cgcatgtgcc ccaagattaa gcaagaggcg gtcccgtcct
     5281 gcacggtcag ccggtcccta gaggcccatt tgagcgctgg accccagctc agcaacggcc
     5341 accggcccaa cacacacgac ttccccctgg ggcggcagct ccccaccagg actaccccta
     5401 cactgagtcc cgaggaactg ctgaacagca gggactgtca ccctggcctg cctcttcccc
     5461 caggattcca tccccatccg gggcccaact accctccttt cctgccagac cagatgcagt
     5521 cacaagtccc ctctctccat tatcaagagc tcatgccacc gggttcctgc ctgccagagg
     5581 agcccaagcc aaagagggga agaaggtcgt ggccccggaa aagaacagcc acccacactt
     5641 gtgactatgc aggctgtggc aaaacctata ccaagagttc tcatctcaag gcacacctgc
     5701 gaactcacac aggcgagaaa ccttaccact gtgactggga cggctgtggg tggaaattcg
     5761 cccgctccga tgaactgacc aggcactacc gcaaacacac agggcaccgg ccctttcagt
     5821 gccagaagtg cgacagggcc ttttccaggt cggaccacct tgccttacac atgaagaggc
     5881 actttggcag cggaaaacag ctgttgaatt ttgaccttct caagttggcg ggagacgtgg
     5941 agtccaaccc agggcccgtc gagatgcccc tcaacgtgaa cttcaccaac aggaactatg
     6001 acctcgacta cgactccgta cagccctatt tcatctgcga cgaggaagag aatttctatc
     6061 accagcaaca gcagagcgag ctgcagccgc ccgcgcccag tgaggatatc tggaagaaat
     6121 tcgagctgct tcccaccccg cccctgtccc cgagccgccg ctccgggctc tgctctccat
     6181 cctatgttgc ggtcgctacg tccttctccc caagggaaga cgatgacggc ggcggtggca
     6241 acttctccac cgccgatcag ctggagatga tgaccgagtt acttggagga gacatggtga
     6301 accagagctt catctgcgat cctgacgacg agaccttcat caagaacatc atcatccagg
     6361 actgtatgtg gagcggtttc tcagccgctg ccaagctggt ctcggagaag ctggcctcct
     6421 accaggctgc gcgcaaagac agcaccagcc tgagccccgc ccgcgggcac agcgtctgct
     6481 ccacctccag cctgtacctg caggacctca ccgccgccgc gtccgagtgc attgacccct
     6541 cagtggtctt tccctacccg ctcaacgaca gcagctcgcc caaatcctgt acctcgtccg
     6601 attccacggc cttctctcct tcctcggact cgctgctgtc ctccgagtcc tccccacggg
     6661 ccagccctga gcccctagtg ctgcatgagg agacaccgcc caccaccagc agcgactctg
     6721 aagaagagca agaagatgag gaagaaattg atgtggtgtc tgtggagaag aggcaaaccc
     6781 ctgccaagag gtcggagtcg ggctcatctc catcccgagg ccacagcaaa cctccgcaca
     6841 gcccactggt cctcaagagg tgccacgtct ccactcacca gcacaactac gccgcacccc
     6901 cctccacaag gaaggactat ccagctgcca agagggccaa gttggacagt ggcagggtcc
     6961 tgaagcagat cagcaacaac cgcaagtgct ccagccccag gtcctcagac acggaggaaa
     7021 acgacaagag gcggacacac aacgtcttgg aacgtcagag gaggaacgag ctgaagcgca
     7081 gcttttttgc cctgcgtgac cagatccctg aattggaaaa caacgaaaag gcccccaagg
     7141 tagtgatcct caaaaaagcc accgcctaca tcctgtccat tcaagcagac gagcacaagc
     7201 tcacctctga aaaggactta ttgaggaaac gacgagaaca gttgaaacac aaactcgaac
     7261 agcttcgaaa ctctggtgca ggcggcgggt ccggaggaga gggcagagga agtcttctaa
     7321 catgcggtga cgtggaggag aatcctggcc caatgggctc ggtgtccaac cagcagtttg
     7381 caggtggctg cgccaaggca gcggagaagg cgccagagga ggcgccgcct gacgcggccc
     7441 gagcggcaga cgagccgcag ctgctgcacg gggccggcat ctgtaagtgg ttcaacgtgc
     7501 gcatggggtt cggcttcctg tctatgaccg cccgcgccgg ggtcgcgctc gaccccccgg
     7561 tggacgtctt tgtgcaccag agcaagctgc acatggaagg gttccgaagc ctcaaggagg
     7621 gtgaggcggt ggagttcacc tttaagaagt ctgccaaggg tctggaatcc atccgtgtca
     7681 ctggccctgg tggtgtgttc tgtattggaa gtgagcggcg gccaaagggg aagaacatgc
     7741 agaagcgaag atccaaagga gacaggtgct acaactgcgg tgggctagac catcatgcca
     7801 aggaatgcaa gctgccaccc cagcccaaga agtgccactt ttgccaaagc atcaaccata
     7861 tggtggcctc gtgtccactg aaggcccagc agggccccag ttctcaggga aagcctgcct
     7921 acttccggga ggaagaggaa gagatccaca gccctgccct gctcccagaa gcccagaatt
     7981 gactcgaggc cggcaaggcc ggatccagac atgataagat acattgatga gtttggacaa
     8041 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     8101 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     8161 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     8221 tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     8281 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8341 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt
     8401 cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     8461 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     8521 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     8581 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     8641 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     8701 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     8761 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     8821 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     8881 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     8941 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     9001 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     9061 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     9121 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     9181 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     9241 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     9301 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     9361 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     9421 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     9481 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     9541 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     9601 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     9661 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     9721 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     9781 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     9841 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     9901 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     9961 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
    10021 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10081 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10141 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
    10201 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
    10261 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
    10321 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
    10381 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
    10441 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
    10501 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
    10561 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
    10621 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
    10681 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
    10741 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
    10801 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
    10861 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
    10921 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
    10981 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
    11041 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
    11101 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac
    11161 caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct
    11221 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
    11281 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
    11341 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
    11401 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
    11461 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
    11521 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
    11581 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
    11641 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc ctcctgcccc
    11701 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
    11761 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
    11821 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
    11881 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
    11941 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
    12001 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
    12061 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
    12121 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
    12181 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
    12241 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
    12301 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
    12361 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
    12421 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
    12481 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
    12541 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    12601 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    12661 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    12721 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    12781 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    12841 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    12901 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
    12961 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    13021 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    13081 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    13141 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    13201 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    13261 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    13321 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    13381 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    13441 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    13501 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    13561 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    13621 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    13681 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    13741 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    13801 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    13861 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    13921 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    13981 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    14041 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    14101 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    14161 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    14221 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    14281 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    14341 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
    14401 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
    14461 cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg
    14521 ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt
    14581 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
    14641 cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat
    14701 atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga
    14761 attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg
    14821 tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca
    14881 gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
    14941 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
    15001 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
    15061 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
    15121 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
    15181 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
    15241 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
    15301 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
    15361 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
    15421 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
    15481 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
    15541 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
    15601 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
    15661 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
    15721 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
    15781 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
    15841 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
    15901 ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg
    15961 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
    16021 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
    16081 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
    16141 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
    16201 atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
    16261 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
    16321 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
    16381 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt
    16441 gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    16501 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
    16561 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
    16621 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
    16681 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
    16741 tagttcatag cccatatatg gagttccg
//
