LOCUS       sequence-212894-       11540 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral backbone expressing mCherry with a 43 basepair insert 
            and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease 
            and APOBEC-mediated base-editing activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati 
            F, Munagala U, Conticello SG, Harris RS
  TITLE     A fluorescent reporter for quantification and enrichment of DNA 
            editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
  JOURNAL   Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 
            10.1093/nar/gky332.
  PUBMED    29746667
REFERENCE   2  (bases 1 to 11540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332."
COMMENT     Sequence Label: ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)
FEATURES             Location/Qualifiers
     source          1..11540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        291..594
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        595..798
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     749..769
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     795..819
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(980..998)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1291..1310
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1610..1663
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1664..1666,1667..1669,1670..2383)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1664..1666,1667..1669,1670..2380)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1709..1730)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1970..1989)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2317..2338
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2455..3088
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4728..4745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4899..5487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4979..4998)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5658..6449,6450..6518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6281..6300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6519..6623)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6691..6709
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6747..6769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6869..6888
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             8581..8838
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9772..9889
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10732..10965
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11150..11194
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             11488..11514
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa tattggagtc
       61 aggagctaaa gaatagtgct gttagcttgc tcaatgccac agccatagca gtagctgagg
      121 ggacagatag ggttatagaa gtagtacaag gagcttgtag agctattcgc cacataccta
      181 gaagaataag acagggcttg gaaaggattt tgctataaga tgggtggcgc ggccgcttaa
      241 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      301 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      361 atgacgtatg ttcccatagt aacgccaatt gggactttcc attgacgtca atgggtggag
      421 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      481 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      541 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      601 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      661 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      721 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      781 gtctatataa gcagagctcg tttagtgaac cgtcagatcc agctggctac cggtatggtg
      841 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      901 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      961 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggcccctc acccttcgcc
     1021 tggggtggcc cctcaccctt cgcctggccc ctcacccttc gcctgggaca tcctgtcccc
     1081 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1141 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1201 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1261 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1321 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1381 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1441 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1501 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1561 cggcggcatg gacgagctgt acaagcccgg tgcagatctc gagtacaagg agggcagagg
     1621 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatggtga gcaagggcga
     1681 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1741 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1801 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1861 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1921 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1981 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2041 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2101 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2161 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2221 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2281 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2341 cgccgccggg atcactctcg gcatggacga gctgtacaag taaggtacct ttaagaccaa
     2401 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     2461 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     2521 gctacttccc tgattggcag aactacacac cagggccagg ggtcagatat ccactgacct
     2581 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     2641 gagagaacac cagcttgtta caccctgtga gcctgcatgg aatggatgac cctgagagag
     2701 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     2761 atccggagta cttcaagaac tgctgacatc gagcttgcta caagggactt tccgctgggg
     2821 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatgctgca
     2881 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     2941 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3001 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3061 ttttagtcag tgtggaaaat ctctagcacc caggaggtag aggttgcagt gagccaagat
     3121 cgcgccactg cattccagcc tgggcaagaa aacaagactg tctaaaataa taataataag
     3181 ttaagggtat taaatatatt tatacatgga ggtcataaaa atatatatat ttgggctggg
     3241 cgcagtggct cacacctgcg cccggccctt tgggaggccg aggcaggtgg atcacctgag
     3301 tttgggagtt ccagaccagc ctgaccaaca tggagaaacc ccttctctgt gtatttttag
     3361 tagattttat tttatgtgta ttttattcac aggtatttct ggaaaactga aactgttttt
     3421 cctctactct gataccacaa gaatcatcag cacagaggaa gacttctgtg atcaaatgtg
     3481 gtgggagagg gaggttttca ccagcacatg agcagtcagt tctgccgcag actcggcggg
     3541 tgtccttcgg ttcagttcca acaccgcctg cctggagaga ggtcagacca cagggtgagg
     3601 gctcagtccc caagacataa acacccaaga cataaacacc caacaggtcc accccgcctg
     3661 ctgcccaggc agagccgatt caccaagacg ggaattagga tagagaaaga gtaagtcaca
     3721 cagagccggc tgtgcgggag aacggagttc tattatgact caaatcagtc tccccaagca
     3781 ttcggggatc agagttttta aggataactt agtgtgtagg gggccagtga gttggagatg
     3841 aaagcgtagg gagtcgaagg tgtccttttg cgccgagtca gttcctgggt gggggccaca
     3901 agatcggatg agccagttta tcaatccggg ggtgccagct gatccatgga gtgcagggtc
     3961 tgcaaaatat ctcaagcact gattgatctt aggttttaca atagtgatgt taccccagga
     4021 acaatttggg gaaggtcaga atcttgtagc ctgtagctgc atgactccta aaccataatt
     4081 tcttttttgt tttttttttt ttatttttga gacagggtct cactctgtca cctaggctgg
     4141 agtgcagtgg tgcaatcaca gctcactgca gcctcaacgt cgtaagctca agcgatcctc
     4201 ccacctcagc ctgcctggta gctgagacta caagcgacgc cccagttaat ttttgtattt
     4261 ttggtagagg cagcgttttg ccgtgtggcc ctggctggtc tcgaactcct gggctcaagt
     4321 gatccagcct cagcctccca aagtgctggg acaaccgggg ccagtcactg cacctggccc
     4381 taaaccataa tttctaatct tttggctaat ttgttagtcc tacaaaggca gtctagtccc
     4441 caggcaaaaa gggggtttgt ttcgggaaag ggctgttact gtctttgttt caaactataa
     4501 actaagttcc tcctaaactt agttcggcct acacccagga atgaacaagg agagcttgga
     4561 ggttagaagc acgatggaat tggttaggtc agatctcttt cactgtctga gttataattt
     4621 tgcaatggtg gttcaaagac tgcccgcttc tgacaccagt cgctgcatta atgaatcggc
     4681 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4741 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4801 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4861 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4921 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4981 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5041 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5101 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5161 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5221 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5281 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5341 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5401 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5461 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5521 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5581 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5641 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5701 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5761 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5821 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5881 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5941 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6001 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6061 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6121 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6181 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6241 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6301 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6361 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6421 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6481 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6541 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6601 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6661 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6721 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     6781 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     6841 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6901 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     6961 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7021 cgccagggga ggcagagatt gcagtaagct gagatcgcag cactgcactc cagcctgggc
     7081 gacagagtaa gactctgtct caaaaataaa ataaataaat caatcagata ttccaatctt
     7141 ttcctttatt tatttattta ttttctattt tggaaacaca gtccttcctt attccagaat
     7201 tacacatata ttctattttt ctttatatgc tccagttttt tttagacctt cacctgaaat
     7261 gtgtgtatac aaaatctagg ccagtccagc agagcctaaa ggtaaaaaat aaaataataa
     7321 aaaataaata aaatctagct cactccttca catcaaaatg gagatacagc tgttagcatt
     7381 aaataccaaa taacccatct tgtcctcaat aattttaagc gcctctctcc accacatcta
     7441 actcctgtca aaggcatgtg ccccttccgg gcgctctgct gtgctgccaa ccaactggca
     7501 tgtggactct gcagggtccc taactgccaa gccccacagt gtgccctgag gctgcccctt
     7561 ccttctagcg gctgccccca ctcggctttg ctttccctag tttcagttac ttgcgttcag
     7621 ccaaggtctg aaactaggtg cgcacagagc ggtaagactg cgagagaaag agaccagctt
     7681 tacagggggt ttatcacagt gcaccctgac agtcgtcagc ctcacagggg gtttatcaca
     7741 ttgcaccctg acagtcgtca gcctcacagg gggtttatca cagtgcaccc ttacaatcat
     7801 tccatttgat tcacaatttt tttagtctct actgtgccta acttgtaagt taaatttgat
     7861 cagaggtgtg ttcccagagg ggaaaacagt atatacaggg ttcagtacta tcgcatttca
     7921 ggcctccacc tgggtcttgg aatgtgtccc ccgaggggtg atgactacct cagttggatc
     7981 tccacaggtc acagtgacac aagataacca agacacctcc caaggctacc acaatgggcc
     8041 gccctccacg tgcacatggc cggaggaact gccatgtcgg aggtgcaagc acacctgcgc
     8101 atcagagtcc ttggtgtgga gggagggacc agcgcagctt ccagccatcc acctgatgaa
     8161 cagaacctag ggaaagcccc agttctactt acaccaggaa aggctggaag ggctaatttg
     8221 gtcccaaaaa agacaagaga tccttgatct gtggatctac cacacacaag gctacttccc
     8281 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     8341 cttcaagtta gtaccagttg aaccagagca agtagaagag gccaatgaag gagagaacaa
     8401 cagcttgtta caccctatga gccagcatgg gatggaggac ccggagggag aagtattagt
     8461 gtggaagttt gacagcctcc tagcatttcg tcacatggcc cgagagctgc atccggagta
     8521 ctacaaagac tgctgacatc gagctttcta caagggactt tccgctgggg actttccagg
     8581 gaggtgtggc ctgggcggga ctggggagtg gcgagccctc agatgctaca tataagcagc
     8641 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg ctcaaagtag
     8761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8821 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag taaagccaga
     8881 ggagatctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     8941 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9001 cgagagcgtc ggtattaagc gggggagaat tagataaatg ggaaaaaatt cggttaaggc
     9061 cagggggaaa gaaacaatat aaactaaaac atatagtatg ggcaagcagg gagctagaac
     9121 gattcgcagt taatcctggc cttttagaga catcagaagg ctgtagacaa atactgggac
     9181 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacaatag
     9241 cagtcctcta ttgtgtgcat caaaggatag atgtaaaaga caccaaggaa gccttagata
     9301 agatagagga agagcaaaac aaaagtaaga aaaaggcaca gcaagcagca gctgacacag
     9361 gaaacaacag ccaggtcagc caaaattacc ctatagtgca gaacctccag gggcaaatgg
     9421 tacatcaggc catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg
     9481 ctttcagccc agaagtaata cccatgtttt cagcattatc agaaggagcc accccacaag
     9541 atttccagta aaaacagtac atacagacaa tggcagcaat ttcaccagta ctacagttaa
     9601 ggccgcctgt tggtgggcgg ggatcaagca ggaatttggc attccctaca atccccaaag
     9661 tcaaggagta atagaatcta tgaataaaga attaaagaaa attataggac aggtaagaga
     9721 tcaggctgaa catcttaaga cagcagtaca aatggcagta ttcatccaca attttaaaag
     9781 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9841 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     9901 cagggacagc agagatccag tttggaaagg accagcaaag ctcctctgga aaggtgaagg
     9961 ggcagtagta atacaagata atagtgacat aaaagtagtg ccaagaagaa aagcaaagat
    10021 catcagggat tatggaaaac agatggcagg tgatgattgt gtggcaagta gacaggatga
    10081 ggattaacac atggaaaaga ttagtaaaac accatatggg attggaagcc ataataagaa
    10141 ttctgcaaca actgctgttt atccatttca gaattgggtg tcgacatagc agaataggcg
    10201 ttactcgaca gaggagagca agaaatggag ccagtagatc ctagactaga gccctggaag
    10261 catccaggaa gtcagcctaa aactgcttgt accaattgct attgtaaaaa gtgttgcttt
    10321 cattgccaag tttgtttcat gacaaaagcc ttaggcatct cctatggcag gaagaagcgg
    10381 agacagcgac gaagagctca tcagaacagt cagactcatc aagcttctct atcaaagcag
    10441 taagtagtac atgggcgcgc ccatgtggca ggaagtagga aaagcaatgt atgcccctcc
    10501 catcagtgga caaattagat gttcatcaaa tattactggg ctgctattaa caagagatgg
    10561 tggtaataac aacaatgggt ccgagatctt cagacctgga ggaggcgata tgagggacaa
    10621 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10681 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    10741 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10801 ggtacaggcc agacaattat tgtctgatat agtgcagcag cagaacaatt tgctgagggc
    10861 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaaac agctccaggc
    10921 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10981 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11041 tctggaacag atttggaata acatgacctg gatggagtgg gacagagaaa ttaacaatta
    11101 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11161 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11221 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11281 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11341 gacccacctc ccaatcccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11401 agagagagac agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga
    11461 cgatctgcgg agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt
    11521 aacgaggatt gtggaacttc
//
