LOCUS       sequence-212896-       15780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    448..496
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     521..540
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        601..980
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        981..1184
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1135..1155
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1231..1340
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      10121..10130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10816..10839
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10861..10890
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10891..10914
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(11004..11024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11422..11433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11658..11792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11695..11714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11749..11768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  11821..11986
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      12170..12624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12257..12276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12466..12487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12650..12754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12755..12823,12824..13615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12973..12992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13786..14374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14275..14294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14528..14545
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  14679..14903
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(14970..15503)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(15692..15697,15698..15714,15715..15720))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
       61 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
      121 cttgcttcag gagatcggta gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
      181 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga
      241 ctctagctat agttctagtg gttggctacg tacccgtagt ggctatggca gggcttgccg
      301 ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tgggggttgg ggtggggaaa
      361 aggaagaaac gcgggcgtat tggtcccaat ggggtctcgg tggggtatcg acagagtgcc
      421 agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacacccgtg cgttttattc
      481 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca
      541 gttagcctcc cccatctccc ggtaccgcat gcggatctgt acgggccaga tatacgcgtt
      601 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      661 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      721 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      781 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      841 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      901 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      961 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     1021 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     1081 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     1141 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
     1201 aacccactgc ttactggctt atcgaatacg gacctttaat tcaacccaac acaatatatt
     1261 atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa
     1321 attacatttt atttacaatc actcgacgga tccttaagaa ggagatatac tatggcgacc
     1381 ctggaaaagc tgatgaaggc cttcgagtcc ctcaagtcct tccaacagca gcaacagcaa
     1441 caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca gccgccaccg
     1501 ccgccgccgc cgccgccgcc tcctcagctt cctcagccgc cgccgcaggc acagccgctg
     1561 ctgcctcagc cgcagccgcc cccgccgccg cccccgccgc cacccggccc ggctgtggct
     1621 gaggagccgc tgcaccgacc aaagaaagaa ctttcagcta ccaagaaaga ccgtgtgaat
     1681 cattgtctga caatatgtga aaacatagtg gcacagtctg tcagaaattc tccagaattt
     1741 cagaaacttc tgggcatcgc tatggaactt tttctgctgt gcagtgatga cgcagagtca
     1801 gatgtcagga tggtggctga cgaatgcctc aacaaagtta tcaaagcttt gatggattct
     1861 aatcttccaa ggttacagct cgagctctat aaggaaatta aaaagaatgg tgcccctcgg
     1921 agtttgcgtg ctgccctgtg gaggtttgct gagctggctc acctggttcg gcctcagaaa
     1981 tgcaggcctt acctggtgaa ccttctgccg tgcctgactc gaacaagcaa gagacccgaa
     2041 gaatcagtcc aggagacctt ggctgcagct gttcccaaaa ttatggcttc ttttggcaat
     2101 tttgcaaatg acaatgaaat taaggttttg ttaaaggcct tcatagcgaa cctgaagtca
     2161 agctccccca ccattcggcg gacagcggct ggatcagcag tgagcatctg ccagcactca
     2221 agaaggacac aatatttcta tagttggcta ctaaatgtgc tcttaggctt actcgttcct
     2281 gtcgaggatg aacactccac tctgctgatt cttggcgtgc tgctcaccct gaggtatttg
     2341 gtgcccttgc tgcagcagca ggtcaaggac acaagcctga aaggcagctt cggagtgaca
     2401 aggaaagaaa tggaagtctc tccttctgca gagcagcttg tccaggttta tgaactgacg
     2461 ttacatcata cacagcacca agaccacaat gttgtgaccg gagccctgga gctgttgcag
     2521 cagctcttca gaacgcctcc acccgagctt ctgcaaaccc tgaccgcagt cgggggcatt
     2581 gggcagctca ccgctgctaa ggaggagtct ggtggccgaa gccgtagtgg gagtattgtg
     2641 gaacttatag ctggaggggg ttcctcatgc agccctgtcc tttcaagaaa acaaaaaggc
     2701 aaagtgctct taggagaaga agaagccttg gaggatgact ctgaatcgag atcggatgtc
     2761 agcagctctg ccttaacagc ctcagtgaag gatgagatca gtggagagct ggctgcttct
     2821 tcaggggttt ccactccagg gtcagcaggt catgacatca tcacagaaca gccacggtca
     2881 cagcacacac tgcaggcgga ctcagtggat ctggccagct gtgacttgac aagctctgcc
     2941 actgatgggg atgaggagga tatcttgagc cacagctcca gccaggtcag cgccgtccca
     3001 tctgaccctg ccatggacct gaatgatggg acccaggcct cgtcgcccat cagcgacagc
     3061 tcccagacca ccaccgaagg gcctgattca gctgttaccc cttcagacag ttctgaaatt
     3121 gtgttagacg gtaccgacaa ccagtatttg ggcctgcaga ttggacagcc ccaggatgaa
     3181 gatgaggaag ccacaggtat tcttcctgat gaagcctcgg aggccttcag gaactcttcc
     3241 atggcccttc aacaggcaca tttattgaaa aacatgagtc actgcaggca gccttctgac
     3301 agcagtgttg ataaatttgt gttgagagat gaagctactg aaccgggtga tcaagaaaac
     3361 aagccttgcc gcatcaaagg tgacattgga cagtccactg atgatgactc tgcacctctt
     3421 gtccattgtg tccgcctttt atctgcttcg tttttgctaa cagggggaaa aaatgtgctg
     3481 gttccggaca gggatgtgag ggtcagcgtg aaggccctgg ccctcagctg tgtgggagca
     3541 gctgtggccc tccacccgga atctttcttc agcaaactct ataaagttcc tcttgacacc
     3601 acggaatacc ctgaggaaca gtatgtctca gacatcttga actacatcga tcatggagac
     3661 ccacaggttc gaggagccac tgccattctc tgtgggaccc tcatctgctc catcctcagc
     3721 aggtcccgct tccacgtggg agattggatg ggcaccatta gaaccctcac aggaaataca
     3781 ttttctttgg cggattgcat tcctttgctg cggaaaacac tgaaggatga gtcttctgtt
     3841 acttgcaagt tagcttgtac agctgtgagg aactgtgtca tgagtctctg cagcagcagc
     3901 tacagtgagt taggactgca gctgatcatc gatgtgctga ctctgaggaa cagttcctat
     3961 tggctggtga ggacagagct tctggaaacc cttgcagaga ttgacttcag gctggtgagc
     4021 tttttggagg caaaagcaga aaacttacac agaggggctc atcattatac agggctttta
     4081 aaactgcaag aacgagtgct caataatgtt gtcatccatt tgcttggaga tgaagacccc
     4141 agggtgcgac atgttgccgc agcatcacta attaggcttg tcccaaagct gttttataaa
     4201 tgtgaccaag gacaagctga tccagtagtg gccgtggcaa gagatcaaag cagtgtttac
     4261 ctgaaacttc tcatgcatga gacgcagcct ccatctcatt tctccgtcag cacaataacc
     4321 agaatatata gaggctataa cctactacca agcataacag acgtcactat ggaaaataac
     4381 ctttcaagag ttattgcagc agtttctcat gaactaatca catcaaccac cagagcactc
     4441 acatttggat gctgtgaagc tttgtgtctt ctttccactg ccttcccagt ttgcatttgg
     4501 agtttaggtt ggcactgtgg agtgcctcca ctgagtgcct cagatgagtc taggaagagc
     4561 tgtaccgttg ggatggccac aatgattctg accctgctct cgtcagcttg gttcccattg
     4621 gatctctcag cccatcaaga tgctttgatt ttggccggaa acttgcttgc agccagtgct
     4681 cccaaatctc tgagaagttc atgggcctct gaagaagaag ccaacccagc agccaccaag
     4741 caagaggagg tctggccagc cctgggggac cgggccctgg tgcccatggt ggagcagctc
     4801 ttctctcacc tgctgaaggt gattaacatt tgtgcccacg tcctggatga cgtggctcct
     4861 ggacccgcaa taaaggcagc cttgccttct ctaacaaacc ccccttctct aagtcccatc
     4921 cgacgaaagg ggaaggagaa agaaccagga gaacaagcat ctgtaccgtt gagtcccaag
     4981 aaaggcagtg aggccagtgc agcttctaga caatctgata cctcaggtcc tgttacaaca
     5041 agtaaatcct catcactggg gagtttctat catcttcctt catacctcaa actgcatgat
     5101 gtcctgaaag ctacacacgc taactacaag gtcacgctgg atcttcagaa cagcacggaa
     5161 aagtttggag ggtttcttcg ctcagccttg gatgttcttt ctcagatact agagctggcc
     5221 acactgcagg acattgggaa gtgtgttgaa gagatcctag gatacctgaa atcctgcttt
     5281 agtcgagaac caatgatggc aactgtttgt gttcaacaat tgttgaagac tctctttggc
     5341 acaaacttgg cctcccagtt tgatggctta tcttccaacc ccagcaagtc acaaggccga
     5401 gcacagcgcc ttggctcctc cagtgtgagg ccaggcttgt accactactg cttcatggcc
     5461 ccgtacaccc acttcaccca ggccctcgct gacgccagcc tgaggaacat ggtgcaggcg
     5521 gagcaggaga acgacacctc gggatggttt gatgtcctcc agaaagtgtc tacccagttg
     5581 aagacaaacc tcacgagtgt cacaaagaac cgtgcagata agaatgctat tcataatcac
     5641 attcgtttgt ttgaacctct tgttataaaa gctttaaaac agtacacgac tacaacatgt
     5701 gtgcagttac agaagcaggt tttagatttg ctggcgcagc tggttcagtt acgggttaat
     5761 tactgtcttc tggattcaga tcaggtgttt attggctttg tattgaaaca gtttgaatac
     5821 attgaagtgg gccagttcag ggaatcagag gcaatcattc caaacatctt tttcttcttg
     5881 gtattactat cttatgaacg ctatcattca aaacagatca ttggaattcc taaaatcatt
     5941 cagctctgtg atggcatcat ggccagtgga aggaaggctg tgacacatgc cataccggct
     6001 ctgcagccca tagtccacga cctctttgta ttaagaggaa caaataaagc tgatgcagga
     6061 aaagagcttg aaacccaaaa agaggtggtg gtgtcaatgt tactgagact catccagtac
     6121 catcaggtgt tggagatgtt cattcttgtc ctgcagcagt gccacaagga gaatgaagac
     6181 aagtggaagc gactgtctcg acagatagct gacatcatcc tcccaatgtt agccaaacag
     6241 cagatgcaca ttgactctca tgaagccctt ggagtgttaa atacattatt tgagattttg
     6301 gccccttcct ccctccgtcc ggtagacatg cttttacgga gtatgttcgt cactccaaac
     6361 acaatggcgt ccgtgagcac tgttcaactg tggatatcgg gaattctggc cattttgagg
     6421 gttctgattt cccagtcaac tgaagatatt gttctttctc gtattcagga gctctccttc
     6481 tctccgtatt taatctcctg tacagtaatt aataggttaa gagatgggga cagtacttca
     6541 acgctagaag aacacagtga agggaaacaa ataaagaatt tgccagaaga aacattttca
     6601 aggtttctat tacaactggt tggtattctt ttagaagaca ttgttacaaa acagctgaag
     6661 gtggaaatga gtgagcagca acatactttc tattgccagg aactaggcac actgctaatg
     6721 tgtctgatcc acatcttcaa gtctggaatg ttccggagaa tcacagcagc tgccactagg
     6781 ctgttccgca gtgatggctg tggcggcagt ttctacaccc tggacagctt gaacttgcgg
     6841 gctcgttcca tgatcaccac ccacccggcc ctggtgctgc tctggtgtca gatactgctg
     6901 cttgtcaacc acaccgacta ccgctggtgg gcagaagtgc agcagacccc gaaaagacac
     6961 agtctgtcca gcacaaagtt acttagtccc cagatgtctg gagaagagga ggattctgac
     7021 ttggcagcca aacttggaat gtgcaataga gaaatagtac gaagaggggc tctcattctc
     7081 ttctgtgatt atgtctgtca gaacctccat gactccgagc acttaacgtg gctcattgta
     7141 aatcacattc aagatctgat cagcctttcc cacgagcctc cagtacagga cttcatcagt
     7201 gccgttcatc ggaactctgc tgccagcggc ctgttcatcc aggcaattca gtctcgttgt
     7261 gaaaaccttt caactccaac catgctgaag aaaactcttc agtgcttgga ggggatccat
     7321 ctcagccagt cgggagctgt gctcacgctg tatgtggaca ggcttctgtg cacccctttc
     7381 cgtgtgctgg ctcgcatggt cgacatcctt gcttgtcgcc gggtagaaat gcttctggct
     7441 gcaaatttac agagcagcat ggcccagttg ccaatggaag aactcaacag aatccaggaa
     7501 taccttcaga gcagcgggct cgctcagaga caccaaaggc tctattccct gctggacagg
     7561 tttcgtctct ccaccatgca agactcactt agtccctctc ctccagtctc ttcccacccg
     7621 ctggacgggg atgggcacgt gtcactggaa acagtgagtc cggacaaaga ctggtacgtt
     7681 catcttgtca aatcccagtg ttggaccagg tcagattctg cactgctgga aggtgcagag
     7741 ctggtgaatc ggattcctgc tgaagatatg aatgccttca tgatgaactc ggagttcaac
     7801 ctaagcctgc tagctccatg cttaagccta gggatgagtg aaatttctgg tggccagaag
     7861 agtgcccttt ttgaagcagc ccgtgaggtg actctggccc gtgtgagcgg caccgtgcag
     7921 cagctccctg ctgtccatca tgtcttccag cccgagctgc ctgcagaacc ggcggcctac
     7981 tggagcaagt tgaatgatct gtttggggat gctgcactgt atcagtccct gcccactctg
     8041 gcccgggccc tggcacagta cctggtggtg gtctccaaac tgcccagtca tttgcacctt
     8101 cctcctgaga aagagaagga cattgtgaaa ttcgtggtgg caacccttga ggccctgtcc
     8161 tggcatttga tccatgagca gatcccgctg agtctggatc tccaggcagg gctggactgc
     8221 tgctgcctgg ccctgcagct gcctggcctc tggagcgtgg tctcctccac agagtttgtg
     8281 acccacgcct gctccctcat ccactgtgtg cacttcatcc tggaggccgt tgcagtgcag
     8341 cctggagagc agcttcttag tccagaaaga aggacaaata ccccaaaagc catcagcgag
     8401 gaggaggagg aagtagatcc aaacacacag aatcctaagt atatcactgc agcctgtgag
     8461 atggtggcag aaatggtgga gtctctgcag tcggtgttgg ccttgggtca taaaaggaat
     8521 agcggcgtgc cggcgtttct cacgccattg ctcaggaaca tcatcatcag cctggcccgc
     8581 ctgccccttg tcaacagcta cacacgtgtg cccccactgg tgtggaagct tggatggtca
     8641 cccaaaccgg gaggggattt tggcacagca ttccctgaga tccccgtgga gttcctccag
     8701 gaaaaggaag tctttaagga gttcatctac cgcatcaaca cactaggctg gaccagtcgt
     8761 actcagtttg aagaaacttg ggccaccctc cttggtgtcc tggtgacgca gcccctcgtg
     8821 atggagcagg aggagagccc accagaagaa gacacagaga ggacccagat caacgtcctg
     8881 gccgtgcagg ccatcacctc actggtgctc agtgcaatga ctgtgcctgt ggccggcaac
     8941 ccagctgtaa gctgcttgga gcagcagccc cggaacaagc ctctgaaagc tctcgacacc
     9001 aggtttggga ggaagctgag cattatcaga gggattgtgg agcaagagat tcaagcaatg
     9061 gtttcaaaga gagagaatat tgccacccat catttatatc aggcatggga tcctgtccct
     9121 tctctgtctc cggctactac aggtgccctc atcagccacg agaagctgct gctgcagatc
     9181 aaccccgagc gggagctggg gagcatgagc tacaaactcg gccaggtgtc catacactcc
     9241 gtgtggctag ggaacagcat cacacccctg agggaggagg aatgggacga ggaagaggag
     9301 gaggaggccg acgcccctgc accttcgtca ccacccacgt ctccagtcaa ctccaggaaa
     9361 caccgggctg gagttgacat ccactcctgt tcgcagtttt tgcttgagtt gtacagccgc
     9421 tggatcctgc cgtccagctc agccaggagg accccggcca tcctgatcag tgaggtggtc
     9481 agatcccttc tagtggtctc agacttgttc accgagcgca accagtttga gctgatgtat
     9541 gtgacgctga cagaactgcg aagggtgcac ccttcagaag acgagatcct cgctcagtac
     9601 ctggtgcctg ccacctgcaa ggcagctgcc gtccttggga tggacaaggc cgtggcggag
     9661 cctgtcagcc gcctgctgga gagcacgctc aggagcagcc acctgcccag cagggttgga
     9721 gccctgcacg gcatcctcta tgtgctggag tgcgacctgc tggacgacac tgccaagcag
     9781 ctcatcccgg tcatcagcga ctatctcctc tccaacctga aagggatcgc ccactgcgtg
     9841 aacattcaca gccagcagca cgtactggtc atgtgtgcca ctgcgtttta cctcattgag
     9901 aactatcctc tggacgtagg gccggaattt tcagcatcaa taatacagat gtgtggggtg
     9961 atgctgtctg gaagtgagga gtccaccccc tccatcattt accactgtgc cctcagaggc
    10021 ctggagcgcc tcctgctctc tgagcagctc tcccgcctgg atgcagaatc gctggtcaag
    10081 ctgagtgtgg acagagtgaa cgtgcacagc ccgcaccggg ccatggcggc tctgggcctg
    10141 atgctcacct gcatgtacac aggaaaggag aaagtcagtc cgggtagaac ttcagaccct
    10201 aatcctgcag cccccgacag cgagtcagtg attgttgcta tggagcgggt atctgttctt
    10261 tttgatagga tcaggaaagg ctttccttgt gaagccagag tggtggccag gatcctgccc
    10321 cagtttctag acgacttctt cccaccccag gacatcatga acaaagtcat cggagagttt
    10381 ctgtccaacc agcagccata cccccagttc atggccaccg tggtgtataa ggtgtttcag
    10441 actctgcaca gcaccgggca gtcgtccatg gtccgggact gggtcatgct gtccctctcc
    10501 aacttcacgc agagggcccc ggtcgccatg gccacgtgga gcctctcctg cttctttgtc
    10561 agcgcgtcca ccagcccgtg ggtcgcggcg atcctcccac atgtcatcag caggatgggc
    10621 aagctggagc aggtggacgt gaaccttttc tgcctggtcg ccacagactt ctacagacac
    10681 cagatagagg aggagctcga ccgcagggcc ttccagtctg tgcttgaggt ggttgcagcc
    10741 ccaggaagcc catatcaccg gctgctgact tgtttacgaa atgtccacaa ggtcaccacc
    10801 tgcggtggaa gcggtgacta caaagacgat gacgacaagt aaaacctcta cttccaatcg
    10861 caccatcatc accatcacca tcaccaccat gattacaagg atgacgacga taagtgaggc
    10921 ggccgccaac ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
    10981 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    11041 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    11101 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    11161 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    11221 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    11281 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    11341 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    11401 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcgggcgc
    11461 ccccctcctc acggcgagcg aagcttgtcg agaagtacta gaggatctct agagcctgca
    11521 gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
    11581 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    11641 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    11701 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    11761 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg
    11821 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
    11881 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
    11941 ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc
    12001 ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg
    12061 gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt
    12121 gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatggga cgcgccctgt
    12181 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    12241 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    12301 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    12361 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    12421 tagacggttt ttcgcccttt gacgttggag tccacgttct taatagtgga ctcttgttcc
    12481 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
    12541 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
    12601 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    12661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    12721 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    12781 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    12841 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    12901 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    12961 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    13021 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    13081 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    13141 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    13201 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    13261 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    13321 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    13381 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    13441 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    13501 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    13561 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    13621 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    13681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    13741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    13801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    13861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    13921 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    13981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    14041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    14101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    14161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    14221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    14281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    14341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    14401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    14461 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    14521 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
    14581 tacgcatctg tgcggtattt cacaccgcat agaccagccg cgtaacctgg caaaatcggt
    14641 tacggttgag taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt
    14701 aaactgaaca aaatagatct aaactatgac aataaagtct taaactagac agaatagttg
    14761 taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa
    14821 agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca
    14881 agggcatggt aaagactata ttcgcggcgt tgtgacaatt taccgaacaa ctccgcggcc
    14941 gggaagccga tctcggcttg aacgaattgt taggtggcgg tacttgggtc gatatcaaag
    15001 tgcatcactt cttcccgtat gcccaacttt gtatagagag ccactgcggg atcgtcaccg
    15061 taatctgctt gcacgtagat cacataagca ccaagcgcgt tggcctcatg cttcaagaga
    15121 ttgatgagcg cggtggcaat gccctgcctc cggtgctcgc cggagactgc gagatcatag
    15181 atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca
    15241 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
    15301 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
    15361 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
    15421 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
    15481 acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
    15541 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
    15601 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
    15661 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
    15721 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
//
