LOCUS       sequence-212901-       15484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation and expression of Csy4 and cogDsRed proteins in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15484)
  AUTHORS   Liang Y, Richardson S, Yan J, Benites VT, Cheng-Yue C, Tran T, 
            Mortimer J, Mukhopadhyay A, Keasling JD, Scheller HV, Loque D
  TITLE     Endoribonuclease-Based Two-Component Repressor Systems for Tight 
            Gene Expression Control in Plants.
  JOURNAL   ACS Synth Biol. 2017 May 19;6(5):806-816. doi: 
            10.1021/acssynbio.6b00295. Epub 2017 Feb 8.
  PUBMED    28094975
REFERENCE   2  (bases 1 to 15484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00295"; journalName: "ACS Synth Biol"; date: 
            "2017-05-19- 19"; volume: "6"; issue: "5"; pages: "806-816"
COMMENT     Sequence Label: pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)
FEATURES             Location/Qualifiers
     source          1..15484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        163..508
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     451..471
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    539..563
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             572..592
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1164..1184)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      1208..1408
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4377..4397
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4401..4420
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             4430..5104
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(4610..4630)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5022..5045
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5106..5130)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5228..5869
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(5877..5894)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5877..5893)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5886..5908)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(6475..6727)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(6923..7717)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7034..7053)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7644..7663
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7747..8127)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    8499..8523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9044..9835
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10081..10669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10570..10589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10823..10840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10855..10995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10921..10940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11081..11103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11339..11533
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11599..12672)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13101..13730)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    15041..15065
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    15230..15251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15266..15271,15272..15289,15290..15296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15304..15320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15309..15331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15328..15344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15328..15344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
       61 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
      121 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
      181 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
      241 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
      301 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
      361 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
      421 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
      481 aggaagttca tttcatttgg agagaacacg ggggactcct agggctagcc tgttatcaac
      541 aagtttgtac aaaaaagcag gcttcatggc tccaaaaaag aaaagaaagg ttgctagcga
      601 ccattactta gatattagat tgagaccaga cccagagttc cctccagcac agttgatgtc
      661 agtgttattc ggaaaattgc accaagcttt ggttgcacaa ggtggagata gaattggtgt
      721 gagttttcct gatttggacg agtctaggtc aagattggga gaaaggttaa gaatccatgc
      781 ttcagcagat gaccttaggg ctttgttagc aagaccttgg ttagagggtc ttagagatca
      841 tttgcagttc ggagaaccag ctgttgtgcc acaccctaca ccatatagac aagtttctag
      901 agtgcaggca aaatcaaatc ctgagagact tagaaggaga ttgatgagga gacacgactt
      961 atctgaagag gaagctagga agagaatacc tgataccgtt gctagagcac ttgacttgcc
     1021 atttgtgact ttgagaagtc aatccactgg tcagcatttt aggttattca ttagacacgg
     1081 accacttcaa gttactgcag aggagggagg attcacctgt tatggattga gtaagggagg
     1141 attcgtgcct tggttctgac acccaacttt tctatacaaa gttgactgac taactaggat
     1201 gagctaagct agctatatca tcaatttatg tattacacat aatatcgcac tcagtctttc
     1261 atctacggca atgtaccagc tgatataatc agttattgaa atatttctga atttaaactt
     1321 gcatcaataa atttatgttt ttgcttggac tataatacct gacttgttat tttatcaata
     1381 aatatttaaa ctatatttct ttcaagatgg gaattaacat ctacaaattg ccttttctta
     1441 tcgaccatgt accccgggta ccaagcttct cgagcggaat gagagacgag agcaatgtgc
     1501 taagagaaga gattgggaag agagaagaga agataaagga aacggaaaag catatggagg
     1561 agcttcacat ggagcaagtg aggctgagaa gacggtcaag tgagcttacg gaagaagtgg
     1621 aaaggacgag agtgtctgca tcggaaatgg ctgagcagaa aagagaagct ataagacagc
     1681 tttgtatgtc tcttgaccat tacagagatg ggtacgacag actttggaga gttgttgcag
     1741 gacataagag taagagagta gtggtcttat caacttgaag tgtaagaaca atgagtcaat
     1801 gactacgtgc aggacattgg acataccgtg tgttcttttg gattgaaatg ttgtttcgaa
     1861 gggctgttag ttgatgttga aaataggttg aagttgaata atgcatgttg atatagtaaa
     1921 tatcaatggt aatattttct catttcccaa aactcaaatg atatcattta actataaact
     1981 aacgtaaact gttgacaata cacttatggt taaaaatttg gagtcttgtt ttagtatacg
     2041 tatcaccacc gcacggtttc aaaaccacat aattgtaaat gttattggaa aatagaactc
     2101 gcaatacgta ttgtattttg gtaaacatag ctctaagcct ctaatatata agctctcaac
     2161 aattctggct aatggtccca agtaagaaaa gcccatgtat tgtaaggtca tgatctcaaa
     2221 aacgagggtg aggtggaata ctaacatgag gagaaagtaa ggtgacaaat ttttggggca
     2281 atagtggtgg atatggtggg gaggtaggta gcatcatttc tccaagtcgc tgtctttcgt
     2341 ggtaatggta ggtgtgtctc tctttatatt atttattact actcattgta aatttctttt
     2401 ttctacaatt tgtttctgac tccaaaatac gtcacaaata taatactagg caaataatta
     2461 ttttattata agtcaataga gtggttgttg taaaattgat tttttgatat tgaaagagtt
     2521 catggacgga tgtgtatgcg ccaaatggta agcccttgta ctgtgccgcg cgtatatttt
     2581 aaccaccact agttgtttct ctttttcaaa aaacacaaaa aaaaaataat ttgttttctt
     2641 aacggcgtca aatctgacgg cgtctcaata cgttcaattt ttttctttct ttcacatggt
     2701 ttctcatagc tttgcattga ccataggtaa agggataagg ataatggttt tttctcttgt
     2761 ttgttttatc cttattattc aaaaaggata aaaaaacagt gatatttaga tttctttgat
     2821 taaaaaagtc attgaaattc atatttgatt ttttgctaaa tgtcaacaca gagacacaaa
     2881 cgtaatgcac tgtcgccaat attcatggat catgacaata aatatcacta gaataattaa
     2941 aaatcagtag aatgcaaaca aagcattttc taagtaaaac agtcttttat attcacgtaa
     3001 ttggaatttc cttttttttt ttttgtcgta attggaattt cctttatcaa acccaaagtc
     3061 caaaacaatc ggcaatgttt tgcaaaatgt tcaaaactat tggcgggttg gtctatccga
     3121 attgaagatc ttttctccat atgatagacc aacgaaattc ggcatacgtg tttttttttt
     3181 tgttttgaaa accctttaaa caaccttaat tcaaaatact aatgtaactt tattgaacgt
     3241 gcatctaaaa attttgaact ttgcttttga gaaataatca atgtaccaat aaagaagatg
     3301 tagtacatac attataatta aatacaaaaa aggaatcacc atatagtaca tggtagacaa
     3361 tgaaaaactt taaaacatat acaatcaata atactctttg tgcataactt tttttgtcgt
     3421 ctcgagttta tatttgagta cttatacaaa ctattagatt acaaactgtg ctcagataca
     3481 ttaagttaat cttatataca agagcactcg agtgttgtcc ttaagttaat cttaagatat
     3541 cttgaggtaa atagaaatag ttgactcgtt tttatcttct tcttttttta ccatgagcaa
     3601 aaaagatgaa ataagttcaa aacgtgacga atctatatgt tactacttag tatgtgtcaa
     3661 tcattaaatc gggaaaactt catcatttca ggagtattac aaaactccta agagtgagaa
     3721 cgactacata gtacatattt tgataaaaga cttgaaaact tgctaaaacg aatttgcgaa
     3781 aatataatca tacaagtgcc agtgattttg atcgaattat tcatagcttt gtaggatgaa
     3841 cttaattaaa taatatctca caaaagtatt gacagtaacc tagtactata ctatctatgt
     3901 tagaatatga ttatgatata atttatcccc tcacttattc atatgatttt tgaagcaact
     3961 actttcgttt ttttaacatt ttcttttgtt ggttattgtt aatgagcata tttagtcgtt
     4021 tcttaattcc actgaaatag aaaatacaaa gagaacttta gttaatagat atgaacataa
     4081 tctcacatcc tcctcctacc ttcaccaaac acttttacat acactttgtg gtctttcttt
     4141 acctaccacc atcaacaaca acaccaagcc ccactcacac acacgcaatc acgttaaatt
     4201 taacgccgtt tattatctca tcattcacca actcccacgt acctaacgcc gtttaccttt
     4261 tgccgttggt cctcatttct caaaccaacc aaacctctcc ctcttataaa atcctctctc
     4321 ccttctttat ttcttcctca gcagcttctt ctgctttcaa ttactctcgc ctaggacaac
     4381 tttgtataat aaagttggcc gttcactgcc gtataggcag ctaagaaata tggcctcctc
     4441 cgagaacgtc atcaccgagt tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg
     4501 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggcc acaacaccgt
     4561 gaagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     4621 gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa
     4681 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4741 ggcgaccgtg acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt
     4801 catcggcgtg aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
     4861 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga cccacaaggc
     4921 cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa
     4981 gaagcccgtg cagctgcccg gctactacta cgtggacgcc aagctggaca tcacctccca
     5041 caacgaggac tacaccatcg tggagcagta cgagcgcacc gagggccgcc accacctgtt
     5101 cctggaccca gctttcttgt acaaagtggt tgataacagc gctgagctct agcacgtgcg
     5161 ggcctgaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt
     5221 gatgcagagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca
     5281 gtttcattgc gcacacacca gaatcctact gagtttgagt attatggcat tgggaaaact
     5341 gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct
     5401 atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt
     5461 cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt
     5521 ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg
     5581 accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt attcactagg
     5641 caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca agtatgtcct
     5701 cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc cttgtcagat
     5761 tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag tatgaaaata
     5821 ttttttaatg cattttatga cttgccaatt gattgacaac atgcatcaag cttggcactg
     5881 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5941 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6001 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgccaat cgaggccgac
     6061 caaccgcaag cgttgtcagt gttgcaaagc gctctgtgtg ggcctacttt aattgcttcc
     6121 agtgttaaat tggcgaaagg caataatatc gcaaaatatt gtgttgtaaa atgtaattat
     6181 gttttaattt catggaaatg tttgagcata atttttatta atgtactaaa ttactgtttt
     6241 gttaaatgca attttgcttt ctcgggattt taatatcaaa atctatttag aaatacacaa
     6301 tattttgttg caggcttgct ggagaatcga tctgctatca taaaaattac aaaaaaattt
     6361 tatttgcctc aattatttta ggattggtat taaggacgct taaattattt gtcgggtcac
     6421 tacgcatcat tgtgattgag aagatcagcg atacgaaata ttcgtagtac tatcgataat
     6481 ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca atacaaagac
     6541 agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga gtaagatcac
     6601 ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa atactcaaac
     6661 cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc caacatggga
     6721 gtccaaggtt tcagggaagc tggaattccg gggtgggcga agaactccag catgagatcc
     6781 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     6841 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     6901 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     6961 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     7021 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     7081 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     7141 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
     7201 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     7261 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     7321 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     7381 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     7441 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     7501 tcgtggccag ccacgatagc cgcgctgcct cgtcctggag ttcattcagg gcaccggaca
     7561 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     7621 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     7681 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatccg gggaattcgc
     7741 tagagtcgat ttggtgtatc gagattggtt atgaaattca gatgctagtg taatgtattg
     7801 gtaatttggg aagatataat aggaagcaag gctatttatc catttctgaa aaggcgaaat
     7861 ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt aaagcgttgt ttggtacact
     7921 tttgactagc gaggcttggc gtgtcagcgt atctattcaa aagtcgttaa tggctgcgga
     7981 tcaagaaaaa gttggaatag aaacagaata cccgcgaaat tcaggcccgg ttgccatgtc
     8041 ctacacgccg aaataaacga ccaaattagt agaaaaataa aaactgactc ggatacttac
     8101 gtcacgtctt gcgcactgat ttgaaaaatc tcaatataaa caaagacggc cacaagaaaa
     8161 aaccaaaaca ccgatattca ttaatcttat ctagtttctc aaaaaaattc atatcttcca
     8221 cacgtgaaaa tgccaatttc tcagacctac ctcggctctg cgaaggcccc cgctggtatc
     8281 aaaagttttt atttcatccg acatggcgcg accgacctca acgagaagga aattgtcgtg
     8341 aacggtgaga agctctgggg cgtgcaaggt tccggaacga acatcggtct caatgcaaaa
     8401 ggggaacgcc aggctctgtt ggcccctcga aattcggcgt taattcagta cattaaaaac
     8461 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     8521 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     8581 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     8641 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     8701 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     8761 aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     8821 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     8881 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     8941 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     9001 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
     9061 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     9121 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     9181 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     9241 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     9301 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     9361 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     9421 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     9481 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     9541 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     9601 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     9661 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     9721 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     9781 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     9841 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     9901 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     9961 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
    10021 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10081 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10141 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10201 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
    10261 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10321 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10381 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10441 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10501 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10561 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10621 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10681 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10741 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10801 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
    10861 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
    10921 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    10981 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    11041 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    11101 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
    11161 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
    11221 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
    11281 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
    11341 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
    11401 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
    11461 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
    11521 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
    11581 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
    11641 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
    11701 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
    11761 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
    11821 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
    11881 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
    11941 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
    12001 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
    12061 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    12121 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    12181 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    12241 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    12301 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    12361 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    12421 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    12481 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    12541 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    12601 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    12661 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    12721 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    12781 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    12841 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    12901 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    12961 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    13021 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    13081 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    13141 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    13201 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    13261 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    13321 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    13381 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    13441 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    13501 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    13561 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    13621 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    13681 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    13741 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    13801 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    13861 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    13921 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    13981 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    14041 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    14101 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    14161 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    14221 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    14281 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    14341 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    14401 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    14461 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    14521 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    14581 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    14641 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    14701 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    14761 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    14821 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    14881 tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttga tcccgagggg
    14941 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
    15001 taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
    15061 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
    15121 tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    15181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    15241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    15301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
    15361 gctcggtacc cggggatcct ctagagggcc cgggacgtcc gcggagatct acgcgtgtcg
    15421 actcgagcgg ccgccagtgt gatggatatc tgcagaattc aggcatggag tcaaagattc
    15481 aaat
//
