LOCUS       sequence-212902-       12887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation and expression of Csy4 and cogDsRed proteins in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Liang Y, Richardson S, Yan J, Benites VT, Cheng-Yue C, Tran T, 
            Mortimer J, Mukhopadhyay A, Keasling JD, Scheller HV, Loque D
  TITLE     Endoribonuclease-Based Two-Component Repressor Systems for Tight 
            Gene Expression Control in Plants.
  JOURNAL   ACS Synth Biol. 2017 May 19;6(5):806-816. doi: 
            10.1021/acssynbio.6b00295. Epub 2017 Feb 8.
  PUBMED    28094975
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.6b00295"; journalName: "ACS Synth Biol"; date: 
            "2017-05-19- 19"; volume: "6"; issue: "5"; pages: "806-816"
COMMENT     Sequence Label: pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)
FEATURES             Location/Qualifiers
     source          1..12887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    318..342
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    507..528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(543..548,549..566,567..573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    581..597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     586..608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     605..621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     605..621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        924..1269
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1212..1232
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1300..1324
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1333..1353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1925..1945)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      1969..2169
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        2315..2494
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    2541..2561
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2565..2584
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             2594..3268
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2774..2794)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3186..3209
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(3270..3294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3392..4033
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(4041..4058)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4041..4057)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4050..4072)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(4639..4891)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(5087..5881)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5198..5217)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5808..5827
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5911..6291)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    6663..6687
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7208..7999
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8245..8833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8734..8753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8987..9004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9019..9159
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9085..9104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9245..9267
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9503..9697
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9763..10836)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11265..11894)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
       61 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
      121 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
      181 ggatcaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
      241 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
      301 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
      361 tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagccaat acgcaaaccg
      421 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      481 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      541 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      601 cacacaggaa acagctatga catgattacg aattcgagct cggtacccgg ggatcctcta
      661 gagggcccgg gacgtccgcg gagatctacg cgtgtcgact cgagcggccg ccagtgtgat
      721 ggatatctgc agaattcagg catggagtca aagattcaaa tagaggacct aacagaactc
      781 gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa
      841 atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
      901 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
      961 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
     1021 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
     1081 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     1141 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     1201 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     1261 gagagaacac gggggactcc tagggctagc ctgttatcaa caagtttgta caaaaaagca
     1321 ggcttcatgg ctccaaaaaa gaaaagaaag gttgctagcg accattactt agatattaga
     1381 ttgagaccag acccagagtt ccctccagca cagttgatgt cagtgttatt cggaaaattg
     1441 caccaagctt tggttgcaca aggtggagat agaattggtg tgagttttcc tgatttggac
     1501 gagtctaggt caagattggg agaaaggtta agaatccatg cttcagcaga tgaccttagg
     1561 gctttgttag caagaccttg gttagagggt cttagagatc atttgcagtt cggagaacca
     1621 gctgttgtgc cacaccctac accatataga caagtttcta gagtgcaggc aaaatcaaat
     1681 cctgagagac ttagaaggag attgatgagg agacacgact tatctgaaga ggaagctagg
     1741 aagagaatac ctgataccgt tgctagagca cttgacttgc catttgtgac tttgagaagt
     1801 caatccactg gtcagcattt taggttattc attagacacg gaccacttca agttactgca
     1861 gaggagggag gattcacctg ttatggattg agtaagggag gattcgtgcc ttggttctga
     1921 cacccaactt ttctatacaa agttgactga ctaactagga tgagctaagc tagctatatc
     1981 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
     2041 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
     2101 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
     2161 tttcaagatg ggaattaaca tctacaaatt gccttttctt atcgaccatg taccccgggt
     2221 accaagcttc tcgaggatac atgagaatta agggagtcac gttatgaccc ccgccgatga
     2281 cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa ggagccactg
     2341 agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca ttattgcgcg
     2401 ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa aatgctccac
     2461 tgacgttcca taaattcccc tcggtatcca attagagtct catattcact ctcaactcga
     2521 tcgaggggat ctacctagga caactttgta taataaagtt ggccgttcac tgccgtatag
     2581 gcagctaaga aatatggcct cctccgagaa cgtcatcacc gagttcatgc gcttcaaggt
     2641 gcgcatggag ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2701 cccctacgag ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt
     2761 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
     2821 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2881 gatgaacttc gaggacggcg gcgtggcgac cgtgacccag gactcctccc tgcaggacgg
     2941 ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtgat
     3001 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     3061 gctgaagggc gagacccaca aggccctgaa gctgaaggac ggcggccact acctggtgga
     3121 gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga
     3181 cgccaagctg gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg
     3241 caccgagggc cgccaccacc tgttcctgga cccagctttc ttgtacaaag tggttgataa
     3301 cagcgctgag ctctagcacg tgcgggcctg aattccagca cactggcggc cgttactagt
     3361 ggatccgagc tcggtaccaa gcttgatgca gagctttcgt tcgtatcatc ggtttcgaca
     3421 acgttcgtca agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttt
     3481 gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac
     3541 tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt
     3601 aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat
     3661 ttgttgtgtg ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa
     3721 tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt
     3781 gtaccattat gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa
     3841 tgttactgaa tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt
     3901 aattttccag aatccttgtc agattctaat cattgcttta taattatagt tatactcatg
     3961 gatttgtagt tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga
     4021 caacatgcat caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4081 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4141 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag
     4201 agcagcttgc caatcgaggc cgaccaaccg caagcgttgt cagtgttgca aagcgctctg
     4261 tgtgggccta ctttaattgc ttccagtgtt aaattggcga aaggcaataa tatcgcaaaa
     4321 tattgtgttg taaaatgtaa ttatgtttta atttcatgga aatgtttgag cataattttt
     4381 attaatgtac taaattactg ttttgttaaa tgcaattttg ctttctcggg attttaatat
     4441 caaaatctat ttagaaatac acaatatttt gttgcaggct tgctggagaa tcgatctgct
     4501 atcataaaaa ttacaaaaaa attttatttg cctcaattat tttaggattg gtattaagga
     4561 cgcttaaatt atttgtcggg tcactacgca tcattgtgat tgagaagatc agcgatacga
     4621 aatattcgta gtactatcga taatttattt gaaaattcat aagaaaagca aacgttacat
     4681 gaattgatga aacaatacaa agacagataa agccacgcac atttaggata ttggccgaga
     4741 ttactgaata ttgagtaaga tcacggaatt tctgacagga gcatgtcttc aattcagccc
     4801 aaatggcagt tgaaatactc aaaccgcccc atatgcagga gcggatcatt cattgtttgt
     4861 ttggttgcct ttgccaacat gggagtccaa ggtttcaggg aagctggaat tccggggtgg
     4921 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     4981 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc
     5041 aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt
     5101 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     5161 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     5221 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     5281 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     5341 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     5401 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     5461 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     5521 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     5581 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     5641 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     5701 ggagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     5761 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     5821 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     5881 tgcgaaacga tccggggaat tcgctagagt cgatttggtg tatcgagatt ggttatgaaa
     5941 ttcagatgct agtgtaatgt attggtaatt tgggaagata taataggaag caaggctatt
     6001 tatccatttc tgaaaaggcg aaatggcgtc accgcgagcg tcacgcgcat tccgttcttg
     6061 ctgtaaagcg ttgtttggta cacttttgac tagcgaggct tggcgtgtca gcgtatctat
     6121 tcaaaagtcg ttaatggctg cggatcaaga aaaagttgga atagaaacag aatacccgcg
     6181 aaattcaggc ccggttgcca tgtcctacac gccgaaataa acgaccaaat tagtagaaaa
     6241 ataaaaactg actcggatac ttacgtcacg tcttgcgcac tgatttgaaa aatctcaata
     6301 taaacaaaga cggccacaag aaaaaaccaa aacaccgata ttcattaatc ttatctagtt
     6361 tctcaaaaaa attcatatct tccacacgtg aaaatgccaa tttctcagac ctacctcggc
     6421 tctgcgaagg cccccgctgg tatcaaaagt ttttatttca tccgacatgg cgcgaccgac
     6481 ctcaacgaga aggaaattgt cgtgaacggt gagaagctct ggggcgtgca aggttccgga
     6541 acgaacatcg gtctcaatgc aaaaggggaa cgccaggctc tgttggcccc tcgaaattcg
     6601 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     6661 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     6721 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     6781 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     6841 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     6901 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc
     6961 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     7021 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     7081 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     7141 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     7201 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     7261 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     7321 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     7381 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     7441 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     7501 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     7561 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     7621 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     7681 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     7741 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     7801 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     7861 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     7921 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     7981 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
     8041 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     8101 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
     8161 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     8401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8941 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9001 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     9061 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     9121 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     9181 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     9241 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     9301 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
     9361 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
     9421 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
     9481 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
     9541 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
     9601 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
     9661 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
     9721 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
     9781 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
     9841 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
     9901 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
     9961 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
    10021 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
    10081 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
    10141 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
    10201 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
    10261 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
    10321 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
    10381 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
    10441 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
    10501 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
    10561 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
    10621 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
    10681 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
    10741 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
    10801 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
    10861 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
    10921 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    10981 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    11041 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    11101 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    11161 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    11221 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    11281 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    11341 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    11401 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    11461 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    11521 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    11581 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    11641 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    11701 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    11761 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    11821 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    11881 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    11941 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    12001 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    12061 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    12121 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    12181 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    12241 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    12301 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    12361 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    12421 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    12481 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    12541 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    12601 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    12661 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    12721 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    12781 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    12841 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaat
//
