LOCUS       sequence-212913-       15281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human soluble CD1D cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Facciotti F, Cavallari M, Angenieux C, Garcia-Alles LF, 
            Signorino-Gelo F, Angman L, Gilleron M, Prandi J, Puzo G, Panza L, 
            Xia C, Wang PG, Dellabona P, Casorati G, Porcelli SA, de la Salle H,
            Mori L, De Libero G
  TITLE     Fine tuning by human CD1e of lipid-specific immune responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14228-33. doi: 
            10.1073/pnas.1108809108. Epub 2011 Aug 15.
  PUBMED    21844346
REFERENCE   2  (bases 1 to 15281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1108809108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-08-23- 23"; volume: "108"; issue: "34"; pages: 
            "14228-33"
COMMENT     Sequence Label: pHCD1Dsol
FEATURES             Location/Qualifiers
     source          1..15281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          696..1268
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             2243..2287
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(2380..2399)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2445..2500
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2499..2518)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3832..4226
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(10970..10989)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11274..11291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11445..12033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11525..11544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12204..12995,12996..13064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12827..12846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13065..13169)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13237..13255
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13708..13853
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13910..14704
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13964..13983)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14574..14593
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(14898..14917)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14942..14989
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
      721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      841 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     1321 tcctcgagat atggggtgcc tgctgtttct gctgctctgg gcgctcctcc aggcttgggg
     1381 aagcgctgaa gtcccgcaaa ggcttttccc cctccgctgc ctccagatct cgtccttcgc
     1441 caatagcagc tggacgcgca ccgacggctt ggcgtggctg ggggagctgc agacgcacag
     1501 ctggagcaac gactcggaca ccgtccgctc tctgaagcct tggtcccagg gcacgttcag
     1561 cgaccagcag tgggagacgc tgcagcatat atttcgggtt tatcgaagca gcttcaccag
     1621 ggacgtgaag gaattcgcca aaatgctacg cttatcctat cccttggagc tccaggtgtc
     1681 cgctggctgt gaggtgcacc ctgggaacgc ctcaaataac ttcttccatg tagcatttca
     1741 aggaaaagat atcctgagtt tccaaggaac ttcttgggag ccaacccaag aggccccact
     1801 ttgggtaaac ttggccattc aagtgctcaa ccaggacaag tggacgaggg aaacagtgca
     1861 gtggctcctt aatggcacct gcccccaatt tgtcagtggc ctccttgagt cagggaagtc
     1921 ggaactgaag aagcaagtga agcccaaggc ctggctgtcc cgtggcccca gtcctggccc
     1981 tggccgtctg ctgctggtgt gccatgtctc aggattctac ccaaagcctg tatgggtgaa
     2041 gtggatgcgg ggtgagcagg agcagcaggg cactcagcca ggggacatcc tgcccaatgc
     2101 tgacgagaca tggtatctcc gagcaaccct ggatgtggtg gctggggagg cagctggcct
     2161 gtcctgtcgg gtgaagcaca gcagtctaga gggccaggac atcgtcctct actggggtgg
     2221 gcctatcgat aagcttggtg gtggtctgaa cgatattttt gaagctcaga aaatcgaatg
     2281 gcatgaatag cggccgcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     2341 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     2401 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     2461 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2521 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     2581 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     2641 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     2701 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     2761 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2821 gagatccctc gacggatcct gagaacttca gggtgagtct atgggaccct tgatgttttc
     2881 tttccccttc ttttctatgg ttaagttcat gtcataggaa ggggagaagt aacagggtac
     2941 agtttagaat gggaaacaga cgaatgattg catcagtgtg gaagtctcag gatcgtttta
     3001 gtttctttta tttgctgttc ataacaattg ttttcttttg tttaattctt gctttctttt
     3061 tttttcttct ccgcaatttt tactattata cttaatgcct taacattgtg tataacaaaa
     3121 ggaaatatct ctgagataca ttaagtaact taaaaaaaaa ctttacacag tctgcctagt
     3181 acattactat ttggaatata tgtgtgctta tttgcatatt cataatctcc ctactttatt
     3241 ttcttttatt tttaattgat acataatcat tatacatatt tatgggttaa agtgtaatgt
     3301 tttaatatgt gtacacatat tgaccaaatc agggtaattt tgcatttgta attttaaaaa
     3361 atgctttctt cttttaatat acttttttgt ttatcttatt tctaatactt tccctaatct
     3421 ctttctttca gggcaataat gatacaatgt atcatgcctc tttgcaccat tctaaagaat
     3481 aacagtgata atttctgggt taaggcaata gcaatatttc tgcatataaa tatttctgca
     3541 tataaattgt aactgatgta agaggtttca tattgctaat agcagctaca atccagctac
     3601 cattctgctt ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc
     3661 cttttgctaa tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg
     3721 gtctgtgtgc tggcccatca ctttggcaaa gaattcaccc caccagtgca ggctgcctat
     3781 cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta agctcgcttt
     3841 cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta ctaaactggg
     3901 ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat ttattttcat
     3961 tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg tgggaggtca
     4021 gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa tacactatat
     4081 cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg caacagcccc
     4141 tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt gatttggagg
     4201 ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc tcatgaatgt
     4261 cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact cagttctctt
     4321 gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc gagatggttt
     4381 ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt ctacttgaag
     4441 aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct gcctccccca
     4501 ctcacagtga cccggaatct gcagtgctag tctcccggaa ctatcactct ttcacagtct
     4561 gctttggaag gactgggctt agtatgaaaa gttaggactg agaagaattt gaaagggggc
     4621 tttttgtagc ttgatattca ctactgtctt attaccctat cataggccca ccccaaatgg
     4681 aagtcccatt cttcctcagg atgtttaaga ttagcattca ggaagagatc agaggtctgc
     4741 tggctccctt atcatgtccc ttatggtgct tctggctctg cagttattag catagtgtta
     4801 ccatcaacca ccttaacttc atttttctta ttcaatacct aggtaggtag atgctagatt
     4861 ctggaaataa aatatgagtc tcaagtggtc cttgtcctct ctcccagtca aattctgaat
     4921 ctagttggca agattctgaa atcaaggcat ataatcagta ataagtgatg atagaagggt
     4981 atatagaaga attttattat atgagagggt gaaatcccag caatttggga ggctgaggca
     5041 ggagaatcgc ttgatcctgg gaggcagagg ttgcagtgag ccaagattgt gccactgcat
     5101 tccagcccag gtgacagcat gagactccgt cacaaaaaaa aaagaaaaaa aagggggggg
     5161 ggggcggtgg agccaagatg accgaatagg aacagctcca gtctatagct cccatcgtga
     5221 gtgacgcaga agacgggtga tttctgcatt tccaactgag gtaccaggtt catctcacag
     5281 ggaagtgcca ggcagtgggt gcaggacagt aggtgcagtg cactgtgcat gagccgaagc
     5341 agggcgaggc atcacctcac ccgggaagca caaggggtca gggaattccc tttcctagtc
     5401 aaagaaaagg gtgacagatg gcacctggaa aatcgggtca ctcccgccct aatactgcgc
     5461 tcttccaaca agctttcttt ggacttagat caatttccct tgggaagaag atttttagca
     5521 cagcaagggg caggatgttc aactgtgaga aaacgaagaa ttagccaaaa aacttccagt
     5581 aagcctgcaa aaaaaaaaaa aaaataaaag ctaagtttct ataaatgttc tgtaaatgta
     5641 aaacagaagg taagtcaact gcacctaata aaaatcactt aatagcaatg tgctgtgtca
     5701 gttgtttatt ggaaccacac ccggtacaca tcctgtccag catttgcagt gcgtgcattg
     5761 aattattgtg ctggctagac ttcatggcgc ctggcaccga atcctgcctt ctcagccaaa
     5821 atgaataatt gctttgttgg caagaaacta agcatcaatg ggacgcgtgc aaagcaccgg
     5881 cggcggtaga tgcggggtaa gtactgaatt ttaattcgac ctatcccggt aaagcgaaag
     5941 cgacacgctt ttttttcaca catagcggga ccgaacacgt tataagtatc gattaggtct
     6001 atttttgtct ctctgtcgga accagaactg gtaaaagttt ccattgcgtc tgggcttgtc
     6061 tatcattgcg tctctatggt ttttggagga ttagacgggc caccagtaat ggtgcatagc
     6121 ggatgtctgt accgccatcg gtgcaccgat ataggtttgg ggctccccaa gggactgctg
     6181 ggatgacagc ttcatattat attgaatggg cgcataatca gcttaattgg tgaggacaag
     6241 ctacaagttg taacctgatc tccacaaagt accgttgccg gtcggggtca aaccgtcttc
     6301 ggtgctcgaa accgccttaa actacagaca ggtcccagcc aagtaggcgg atcaaaacct
     6361 caaaaaggcg ggagccaatc aaaatgcagc attatatttt aagctcaccg aaaccggtaa
     6421 gtaaagacta tgtatttttt cccagtgaat aattgttgtt aacaataatc acaccatcac
     6481 cgttttttca agcgggaaaa aatagccagc taactataaa aagctgctga cagaccccgg
     6541 ttttcacatg gacctgaaac cttttgcaag aaccaatcca ttctcagggt tggattgtct
     6601 gtggtgcaga gagcctctta cagaagttga tgcttttagg tgcatggtca aagactttca
     6661 tgttgtaatt cgggaaggct gtagatatgg tgcatgtacc atttgtcttg aaaactgttt
     6721 agctactgaa agaagacttt ggcaaggtgt tccagtaaca ggtgaggaag ctgaattatt
     6781 gcatggcaaa acacttgata ggctttgcat aagatgctgc tactgtgggg gcaaactaac
     6841 aaaaaatgaa aaacatcggc atgtgctttt taatgagcct ttctgcaaaa ccagagctaa
     6901 cataattaga ggacgctgct acgactgctg cagacatggt tcaaggtcca aatacccata
     6961 gaaacttgga tgattcacct gcaggaccgt tgctgatttt aagtccatgt gcaggcacac
     7021 ctaccaggtc tcctgcagca cctgatgcac ctgatttcag acttccgtgc catttcggcc
     7081 gtcctactag gaagcgaggt cccactacgc ctccgctttc ctctcccgga aaactgtgtg
     7141 caacagggcc acgtcgagtg tattctgtga ctgtctgctg tggaaactgc ggaaaagagc
     7201 tgacttttgc tgtgaagacc agctcgacgt ccctgcttgg atttgaacac cttttaaact
     7261 cagatttaga cctcttgtgt ccacgttgtg aatctcgcga gcgtcatggc aaacgataaa
     7321 ggtagcaatt gggattcggg cttgggatgc tcatatctgc tgactgaggc agaatgtgaa
     7381 agtgacaaag agaatgagga acccggggca ggtgtagaac tgtctgtgga atctgatcgg
     7441 tatgatagcc aggatgagga ttttgttgac aatgcatcag tctttcaggg aaatcacctg
     7501 gaggtcttcc aggcattaga gaaaaaggcg ggtgaggagc agattttaaa tttgaaaaga
     7561 aaagtattgg ggagttcgca aaacagcagc ggttccgaag catctgaaac tccagttaaa
     7621 agacggaaat caggagcaaa gcgaagatta tttgctgaaa atgaagctaa ccgtgttctt
     7681 acgcccctcc aggtacaggg ggagggggag gggaggcaag aacttaatga ggagcaggca
     7741 attagtcatc tacatctgca gcttgttaaa tctaaaaatg ctacagtttt taagctgggg
     7801 ctctttaaat ctttgttcct ttgtagcttc catgatatta cgaggttgtt taagaatgat
     7861 aagaccacta atcagcaatg ggtgctggct gtgtttggcc ttgcagaggt gttttttgag
     7921 gcgagtttcg aactcctaaa gaagcagtgt agttttctgc agatgcaaaa aagatctcat
     7981 gaaggaggaa cttgtgcagt ttacttaatc tgctttaaca cagctaaaag cagagaaaca
     8041 gtccggaatc tgatggcaaa catgctaaat gtaagagaag agtgtttgat gctgcagcca
     8101 cctaaaattc gaggactcag cgcagctcta ttctggttta aaagtagttt gtcacccgct
     8161 acacttaaac atggtgcttt acctgagtgg atacgggcgc aaactactct gaacgagagc
     8221 ttgcagaccg agaaattcga cttcggaact atggtgcaat gggcctatga tcacaaatat
     8281 gctgaggagt ctaaaatagc ctatgaatat gctttggctg caggatctga tagcaatgca
     8341 cgggcttttt tagcaactaa cagccaagct aagcatgtga aggactgtgc aactatggta
     8401 agacactatc taagagctga aacacaagca ttaagcatgc ctgcatatat taaagctagg
     8461 tgcaagctgg caactgggga aggaagctgg aagtctatcc taactttttt taactatcag
     8521 aatattgaat taattacctt tattaatgct ttaaagctct ggctaaaagg aattccaaaa
     8581 aaaaactgtt tagcatttat tggccctcca aacacaggca agtctatgct ctgcaactca
     8641 ttaattcatt ttttgggtgg tagtgtttta tcttttgcca accataaaag tcacttttgg
     8701 cttgcttccc tagcagatac tagagctgct ttagtagatg atgctactca tgcttgctgg
     8761 aggtactttg acacatacct cagaaatgca ttggatggct accctgtcag tattgataga
     8821 aaacacaaag cagcggttca aattaaagct ccacccctcc tggtaaccag taatattgat
     8881 gtgcaggcag aggacagata tttgtacttg catagtcggg tgcaaacctt tcgctttgag
     8941 cagccatgca cagatgaatc gggtgagcaa ccttttaata ttactgatgc agattggaaa
     9001 tctttttttg taaggttatg ggggcgttta gacctgattg acgaggagga ggatagtgaa
     9061 gaggatggag acagcatgcg aacgtttaca tgtagcgcaa gaaacacaaa tgcagttgat
     9121 tgagaaaagt agtgataagt tgcaagatca tatactgtac tggactgctg ttagaactga
     9181 gaacacactg ctttatgctg caaggaaaaa aggggtgact gtcctaggac actgcagagt
     9241 accacactct gtagtttgtc aagagagagc caagcaggcc attgaaatgc agttgtcttt
     9301 gcaggagtta agcaaaactg agtttgggga tgaaccatgg tctttgcttg acacaagctg
     9361 ggaccgatat atgtcagaac ctaaacggtg ctttaagaaa ggcgccaggg tggtagaggt
     9421 ggagtttgat ggaaatgcaa gcaatacaaa ctggtacact gtctacagca atttgtacat
     9481 gcgcacagag gacggctggc agcttgcgaa ggctggggct gacggaactg ggctctacta
     9541 ctgcaccatg gccggtgctg gacgcattta ctattctcgc tttggtgacg aggcagccag
     9601 atttagtaca acagggcatt actctgtaag agatcaggac agagtgtatg ctggtgtctc
     9661 atccacctct tctgatttta gagatcgccc agacggagtc tgggtcgcat ccgaaggacc
     9721 tgaaggagac cctgcaggaa aagaagccga gccagcccag cctgtctctt ctttgctcgg
     9781 ctcccccgcc tgcggtccca tcagagcagg cctcggttgg gtacgggacg gtcctcgctc
     9841 gcacccctac aattttcctg caggctcggg gggctctatt ctccgctctt cctccacccc
     9901 ggtgcagggc acggtaccgg tggacttggc atcaaggcag gaagaagagg agcagtcgcc
     9961 cgactccaca gaggaagaac cagtgactct cccaaggcgc accaccaatg atggattcca
    10021 cctgttaaag gcaggagggt catgctttgc tctaatttca ggaactgcta accaggtaaa
    10081 gtgctatcgc tttcgggtga aaaagaacca tagacatcgc tacgagaact gcaccaccac
    10141 ctggttcaca gttgctgaca acggtgctga aagacaagga caagcacaaa tactgatcac
    10201 ctttggatcg ccaagtcaaa ggcaagactt tctgaaacat gtaccactac ctcctggaat
    10261 gaacatttcc ggctttacag ccagcttgga cttctgatca ctgccattgc cttttcttca
    10321 tctgactggt gtactatgcc aaatctatgg tttctattgt tcttgggact agttgctgca
    10381 atgcaactgc tgctattact gttcttactc ttgttttttc ttgtatactg ggatcatttt
    10441 gagtgctcct gtacaggtct gcccttttaa tgcctttaca tcactggcta ttggctgtgt
    10501 ttttactgtt gtgtggattt gttttatata ctgtatgaag ttttttcatt tgtgcttgta
    10561 ttgctgtttg taagtttttt actagagttt gtattccccc tgctcagatt ttatatggtt
    10621 taagctgcag caataaaaat gagtgcacga aaaagagtaa aacgtgccag tgcctatgac
    10681 ctgtacagga catgcaagca agcgggcaca tgtccaccag atgtgatacc aaaggtagaa
    10741 ggagatacta tagcagataa aattttgaaa tttgggggtc ttgcaatcta cttaggaggg
    10801 ctaggaatag gaacatggtc tactggaagg gttgctgcag gtggatcacc aaggtacaca
    10861 ccactccgaa cagcagggtc cacatcatcg cttgcatcaa taggatcgat cctctacgcc
    10921 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
    10981 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
    11041 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
    11101 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
    11161 caggagtcgc ataagggaga gcgtcgagcg gctgcgcggc cggccgctat gcggtgtgaa
    11221 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
    11281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11641 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11881 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12481 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
    12541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
    12841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    13021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    13261 cgtcttcaag aattctcatg tttgacagct tatcatcgat aagcttcacg ctgccgcaag
    13321 cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa
    13381 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
    13441 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
    13501 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
    13561 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
    13621 agatcctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
    13681 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
    13741 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
    13801 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
    13861 aacagcgtgc cgcagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
    13921 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    13981 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    14041 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
    14101 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    14161 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    14221 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    14281 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    14341 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    14401 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
    14461 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    14521 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    14581 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    14641 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    14701 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    14761 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    14821 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccggg
    14881 agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
    14941 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
    15001 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    15061 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    15121 gcagccaacg tcggggcggc aagccctgcc atagccacgg gccccgtggg ttagggacgg
    15181 cggatcgcgg ccctctagag agcttggccc attgcatacg ttgtatccat atcataatat
    15241 gtacatttat attggctcat gtccaacatt accgccatgt t
//
