LOCUS       sequence-212928-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of SFXN5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_SFXN5
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             23..616
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    679..1036
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1095..1511
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1441..1463
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1479..1495
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1521..1895
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1833..1849
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2032..2043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2970..3538
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2988..3540
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3132..3149)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3359..3378
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3552..3947
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4118..4587
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4717..4734)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4888..5476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4968..4987)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5640..6431,6432..6500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6263..6282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6501..6605)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctgc ataatgaaag accccacctg taggtttggc aagctagctt
       61 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
      121 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      181 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
      241 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
      301 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      361 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      421 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      481 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
      541 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
      601 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
      661 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
      721 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
      781 gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc
      841 cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg
      901 gactctttgg tgcacccccc taataggagg gatatgtggt tctggtagga gacgagaacc
      961 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg
     1021 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     1081 tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga
     1141 aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta
     1201 ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta
     1261 accgagacct catcacccag gttaagatca aggtcttttc acctggcccg catggacacc
     1321 cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg
     1381 tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc
     1441 cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt
     1501 ctctaggcgc ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa
     1561 acttccctga ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac
     1621 aggctctcta cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac
     1681 aactggaccg accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc
     1741 gacaccagac taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca
     1801 cccccaccgc cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg
     1861 ctgccgaccc cgggggtgga ccatcctcta gactgccgga tctagctagt taattaagga
     1921 tccgccacca tggcggatac agcgactaca gcatcggcgg cggcggctag tgccgctagc
     1981 gcctcgagcg atgcacctcc tttccaactg ggcaaacccc gcttccagca gacgtccttc
     2041 tatggccgct tcaggcactt cttggatatc atcgaccctc gcacactctt tgtcactgag
     2101 agacgtctca gagaggctgt gcagctgctg gaggactata agcatgggac cctgcgcccg
     2161 ggggtcacca atgaacagct ctggagtgca cagaaaatca agcaggctat tctacatccg
     2221 gacaccaatg agaagatctt catgccattt agaatgtcag gttatattcc ttttgggacg
     2281 ccaattgtag tcggtcttct cttgcccaac cagacactgg catccactgt cttctggcag
     2341 tggctgaacc agagccacaa tgcctgtgtc aactatgcaa accgcaatgc gaccaagcct
     2401 tcacctgcat ccaagttcat ccagggatac ctgggagctg tcatcagcgc tgtctccatt
     2461 gctgtgggcc ttaatgtcct ggttcagaaa gccaacaagt tcaccccagc cacccgcctt
     2521 ctcatccaga ggtttgtgcc gttccctgct gtagccagtg ccaatatctg caatgtggtc
     2581 ctgatgcggt acggggagct ggaggaaggg attgatgtcc tggacagcga tggcaacctc
     2641 gtgggctcct ccaagatcgc agcccgacac gccctgctgg agacggcgct gacgcgagtg
     2701 gtcctgccca tgcccatcct ggtgctaccc ccgatcgtca tgtccatgct ggagaagacg
     2761 gctctcctgc aggcacgccc ccggctgctc ctccctgtgc aaagcctcgt gtgcctggca
     2821 gccttcggcc tggccctgcc gctggccatc agcctcttcc cgcaaatgtc agagattgaa
     2881 acatcccaat tagagccgga gatagcccag gccacgagca gccggacagt ggtgtacaac
     2941 aaggggttgt gagcggccgc tacgtaaatt ccgccccccc ccccctaacg ttactggccg
     3001 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3061 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3121 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3181 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3241 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3301 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3361 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3421 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     3481 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3541 tggccacaac catggttcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     3601 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     3661 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     3721 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     3781 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     3841 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     3901 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagtc gacgataaaa
     3961 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     4021 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     4081 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     4141 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     4201 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     4261 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     4321 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     4381 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     4441 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     4501 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     4561 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     4621 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     4681 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4741 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4801 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4861 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4921 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4981 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5041 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5101 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5161 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5221 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5281 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5341 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5401 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5461 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5521 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5581 ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5641 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5701 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5761 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5821 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5881 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5941 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6001 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6061 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6121 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6181 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6241 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6301 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6361 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6421 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6481 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6541 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6601 ccgcgcacat tt
//
