LOCUS       sequence-212945-        9325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses catalytically inactive human APOBEC3A with an L1 intron 
            fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear 
            localization signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati 
            F, Munagala U, Conticello SG, Harris RS
  TITLE     A fluorescent reporter for quantification and enrichment of DNA 
            editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
  JOURNAL   Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 
            10.1093/nar/gky332.
  PUBMED    29746667
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332."
COMMENT     Sequence Label: A3Ai E72A-Cas9n-UGI-NLS
FEATURES             Location/Qualifiers
     source          1..9325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(1937..5335,5336..5338,5339..5935,5936,5937..5939,
                     5940..5941,5942..5944,5945..6037)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1937..1960,1961..1963,1964..6037)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6050..6298
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6311..6331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6340..6357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6380..6397)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6386..6610
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6681..6697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6681..6697)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6694..6716)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6705..6721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6729..6735,6736..6753,6754..6759))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6774..6795
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6912..6929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7083..7671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7163..7182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7842..8633,8634..8702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8465..8484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8703..8807)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8886..8905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9077..9325,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgat ggaagccagc ccagcatccg ggcccagaca
      421 cttgatggat ccacacatat tcacttccaa ctttaacaat ggcattggaa ggcataagac
      481 ctacctgtgc tacgaagtgg agcgcctgga caatggcacc tcggtcaaga tggaccagca
      541 caggggcttt ctacacaacc aggtgagtcc aggagatgtt tcagcactgt tgcctttagt
      601 ctcgaggcaa cttagacaac tgagtattga tctgagcaca gcagggtgtg agctgtttga
      661 agatactggg gttgggggtg aagaaactgc agaggactaa ctgggctgag acccagtggc
      721 aatgttttag ggcctaagga atgcctctga aaatctagat ggacaacttt gactttgaga
      781 aaagagaggt ggaaatgagg aaaatgactt ttctttatta gatttcggta gaaagaactt
      841 tcatctttcc cctatttttg ttattcgttt taaaacatct atctggaggc aggacaagta
      901 tggccattaa aaagatgcag gcagaaggca tatattggct cagtcaaagt ggggaacttt
      961 ggtggccaaa catacattgc taaggctatt cctatatcag ctggacacat ataaaatgct
     1021 gctaatgctt cattacaaac ttatatcctt taattccaga tgggggcaaa gtatgtccag
     1081 gggtgaggaa caattgaaac atttgggctg gagtagattt tgaaagtcag ctctgtgtgt
     1141 gtgtgtgtgt gtgtgtgtgt gtgtgnncgc gcacgtgtgt ttgtgtgtgt gtgagagcgt
     1201 gtgtttcttt taacgttttc agcctacagc atacagggtt catggtggca agaagataac
     1261 aagatttaaa ttatggccag tgactagtgc tgcaagaaga acaactacct gcatttaatg
     1321 ggaaagcaaa atctcaggct ttgagggaag ttaacatagg cttgattctg ggtggaagct
     1381 gggtgtgtag ttatctggag gccaggctgg agctctcagc tcactatggg ttcatcttta
     1441 ttgtctcctt tcatctcaac aggctaagaa tcttctctgt ggcttttacg gccgccatgc
     1501 ggcgctgcgc ttcttggacc tggttccttc tttgcagttg gacccggccc agatctacag
     1561 ggtcacttgg ttcatctcct ggagcccctg cttctcctgg ggctgtgccg gggaagtgcg
     1621 tgcgttcctt caggagaaca cacacgtgag actgcgcatc ttcgctgccc gcatctatga
     1681 ttacgacccc ctatataagg aggcgctgca aatgctgcgg gatgctgggg cccaagtctc
     1741 catcatgacc tacgatgaat ttaagcactg ctgggacacc tttgtggacc accagggatg
     1801 tcccttccag ccctgggatg gactagatga gcacagccaa gccctgagtg ggaggctgcg
     1861 ggccattctc cagaatcagg gaaacggaag cggcagcgag actcccggga cctcagagtc
     1921 cgccacaccc gaaagtgata aaaagtattc tattggttta gccatcggca ctaattccgt
     1981 tggatgggct gtcataaccg atgaatacaa agtaccttca aagaaattta aggtgttggg
     2041 gaacacagac cgtcattcga ttaaaaagaa tcttatcggt gccctcctat tcgatagtgg
     2101 cgaaacggca gaggcgactc gcctgaaacg aaccgctcgg agaaggtata cacgtcgcaa
     2161 gaaccgaata tgttacttac aagaaatttt tagcaatgag atggccaaag ttgacgattc
     2221 tttctttcac cgtttggaag agtccttcct tgtcgaagag gacaagaaac atgaacggca
     2281 ccccatcttt ggaaacatag tagatgaggt ggcatatcat gaaaagtacc caacgattta
     2341 tcacctcaga aaaaagctag ttgactcaac tgataaagcg gacctgaggt taatctactt
     2401 ggctcttgcc catatgataa agttccgtgg gcactttctc attgagggtg atctaaatcc
     2461 ggacaactcg gatgtcgaca aactgttcat ccagttagta caaacctata atcagttgtt
     2521 tgaagagaac cctataaatg caagtggcgt ggatgcgaag gctattctta gcgcccgcct
     2581 ctctaaatcc cgacggctag aaaacctgat cgcacaatta cccggagaga agaaaaatgg
     2641 gttgttcggt aaccttatag cgctctcact aggcctgaca ccaaatttta agtcgaactt
     2701 cgacttagct gaagatgcca aattgcagct tagtaaggac acgtacgatg acgatctcga
     2761 caatctactg gcacaaattg gagatcagta tgcggactta tttttggctg ccaaaaacct
     2821 tagcgatgca atcctcctat ctgacatact gagagttaat actgagatta ccaaggcgcc
     2881 gttatccgct tcaatgatca aaaggtacga tgaacatcac caagacttga cacttctcaa
     2941 ggccctagtc cgtcagcaac tgcctgagaa atataaggaa atattctttg atcagtcgaa
     3001 aaacgggtac gcaggttata ttgacggcgg agcgagtcaa gaggaattct acaagtttat
     3061 caaacccata ttagagaaga tggatgggac ggaagagttg cttgtaaaac tcaatcgcga
     3121 agatctactg cgaaagcagc ggactttcga caacggtagc attccacatc aaatccactt
     3181 aggcgaattg catgctatac ttagaaggca ggaggatttt tatccgttcc tcaaagacaa
     3241 tcgtgaaaag attgagaaaa tcctaacctt tcgcatacct tactatgtgg gacccctggc
     3301 ccgagggaac tctcggttcg catggatgac aagaaagtcc gaagaaacga ttactccatg
     3361 gaattttgag gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg agaggatgac
     3421 caactttgac aagaatttac cgaacgaaaa agtattgcct aagcacagtt tactttacga
     3481 gtatttcaca gtgtacaatg aactcacgaa agttaagtat gtcactgagg gcatgcgtaa
     3541 acccgccttt ctaagcggag aacagaagaa agcaatagta gatctgttat tcaagaccaa
     3601 ccgcaaagtg acagttaagc aattgaaaga ggactacttt aagaaaattg aatgcttcga
     3661 ttctgtcgag atctccgggg tagaagatcg atttaatgcg tcacttggta cgtatcatga
     3721 cctcctaaag ataattaaag ataaggactt cctggataac gaagagaatg aagatatctt
     3781 agaagatata gtgttgactc ttaccctctt tgaagatcgg gaaatgattg aggaaagact
     3841 aaaaacatac gctcacctgt tcgacgataa ggttatgaaa cagttaaaga ggcgtcgcta
     3901 tacgggctgg ggacgattgt cgcggaaact tatcaacggg ataagagaca agcaaagtgg
     3961 taaaactatt ctcgattttc taaagagcga cggcttcgcc aataggaact ttatgcagct
     4021 gatccatgat gactctttaa ccttcaaaga ggatatacaa aaggcacagg tttccggaca
     4081 aggggactca ttgcacgaac atattgcgaa tcttgctggt tcgccagcca tcaaaaaggg
     4141 catactccag acagtcaaag tagtggatga gctagttaag gtcatgggac gtcacaaacc
     4201 ggaaaacatt gtaatcgaga tggcacgcga aaatcaaacg actcagaagg ggcaaaaaaa
     4261 cagtcgagag cggatgaaga gaatagaaga gggtattaaa gaactgggca gccagatctt
     4321 aaaggagcat cctgtggaaa atacccaatt gcagaacgag aaactttacc tctattacct
     4381 acaaaatgga agggacatgt atgttgatca ggaactggac ataaaccgtt tatctgatta
     4441 cgacgtcgat cacattgtac cccaatcctt tttgaaggac gattcaatcg acaataaagt
     4501 gcttacacgc tcggataaga accgagggaa aagtgacaat gttccaagcg aggaagtcgt
     4561 aaagaaaatg aagaactatt ggcggcagct cctaaatgcg aaactgataa cgcaaagaaa
     4621 gttcgataac ttaactaaag ctgagagggg tggcttgtct gaacttgaca aggccggatt
     4681 tattaaacgt cagctcgtgg aaacccgcca aatcacaaag catgttgcac agatactaga
     4741 ttcccgaatg aatacgaaat acgacgagaa cgataagctg attcgggaag tcaaagtaat
     4801 cactttaaag tcaaaattgg tgtcggactt cagaaaggat tttcaattct ataaagttag
     4861 ggagataaat aactaccacc atgcgcacga cgcttatctt aatgccgtcg tagggaccgc
     4921 actcattaag aaatacccga agctagaaag tgagtttgtg tatggtgatt acaaagttta
     4981 tgacgtccgt aagatgatcg cgaaaagcga acaggagata ggcaaggcta cagccaaata
     5041 cttcttttat tctaacatta tgaatttctt taagacggaa atcactctgg caaacggaga
     5101 gatacgcaaa cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg tatgggataa
     5161 gggccgggac ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca acatagtaaa
     5221 gaaaactgag gtgcagaccg gagggttttc aaaggaatcg attcttccaa aaaggaatag
     5281 tgataagctc atcgctcgta aaaaggactg ggacccgaaa aagtacggtg gcttcgatag
     5341 ccctacagtt gcctattctg tcctagtagt ggcaaaagtt gagaagggaa aatccaagaa
     5401 actgaagtca gtcaaagaat tattggggat aacgattatg gagcgctcgt cttttgaaaa
     5461 gaaccccatc gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg atctcataat
     5521 taaactacca aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga tgttggctag
     5581 cgccggagag cttcaaaagg ggaacgaact cgcactaccg tctaaatacg tgaatttcct
     5641 gtatttagcg tcccattacg agaagttgaa aggttcacct gaagataacg aacagaagca
     5701 actttttgtt gagcagcaca aacattatct cgacgaaatc atagagcaaa tttcggaatt
     5761 cagtaagaga gtcatcctag ctgatgccaa tctggacaaa gtattaagcg catacaacaa
     5821 gcacagggat aaacccatac gtgagcaggc ggaaaatatt atccatttgt ttactcttac
     5881 caacctcggc gctccagccg cattcaagta ttttgacaca acgatagatc gcaaacgata
     5941 cacttctacc aaggaggtgc tagacgcgac actgattcac caatccatca cgggattata
     6001 tgaaactcgg atagatttgt cacagcttgg gggtgactct ggtggttcta ctaatctgtc
     6061 agatattatt gaaaaggaga ccggtaagca actggttatc caggaatcca tcctcatgct
     6121 cccagaggag gtggaagaag tcattgggaa caagccggaa agcgatatac tcgtgcacac
     6181 cgcctacgac gagagcaccg acgagaatgt catgcttctg actagcgacg cccctgaata
     6241 caagccttgg gctctggtca tacaggatag caacggtgag aacaagatta agatgctctc
     6301 tggtggttct cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga
     6361 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6421 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6481 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6541 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6601 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc
     6661 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6721 attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg
     6781 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6841 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6901 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6961 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7021 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7081 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7141 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7201 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7261 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7321 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7381 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7441 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7501 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7561 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7621 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7681 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7741 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7801 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7861 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7921 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7981 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8041 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8101 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8161 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8221 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8281 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8341 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8401 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8461 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8521 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8581 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8641 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8701 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8761 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8821 aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact
     8881 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     8941 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9001 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     9061 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     9121 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     9181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     9241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9301 cacttggcag tacatcaagt gtatc
//
