LOCUS       sequence-212947-        9325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human APOBEC3A containing an L1 intron fused to Cas9n, 
            Uracil DNA Glycosylase Inhibitor, with a nuclear localization 
            signal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   St Martin A, Salamango D, Serebrenik A, Shaban N, Brown WL, Donati 
            F, Munagala U, Conticello SG, Harris RS
  TITLE     A fluorescent reporter for quantification and enrichment of DNA 
            editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
  JOURNAL   Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 
            10.1093/nar/gky332.
  PUBMED    29746667
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332."
COMMENT     Sequence Label: A3Ai-Cas9n-UGI-NLS
FEATURES             Location/Qualifiers
     source          1..9325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        502..705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     656..676
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(2307..5705,5706..5708,5709..6305,6306,6307..6309,
                     6310..6311,6312..6314,6315..6407)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(2307..2330,2331..2333,2334..6407)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6420..6668
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6681..6701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6710..6727
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6750..6767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6756..6980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7051..7067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7051..7067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7064..7086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7075..7091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7099..7105,7106..7123,7124..7129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7144..7165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7282..7299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7453..8041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7533..7552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8212..9003,9004..9072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8835..8854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9073..9177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9256..9275)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
       61 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      121 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      181 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      241 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      301 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      361 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      421 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      481 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      541 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      601 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      661 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      721 cagatccgct agagatccgc ggccgcgccg ccaccatgat ggaagccagc ccagcatccg
      781 ggcccagaca cttgatggat ccacacatat tcacttccaa ctttaacaat ggcattggaa
      841 ggcataagac ctacctgtgc tacgaagtgg agcgcctgga caatggcacc tcggtcaaga
      901 tggaccagca caggggcttt ctacacaacc aggtgagtcc aggagatgtt tcagcactgt
      961 tgcctttagt ctcgaggcaa cttagacaac tgagtattga tctgagcaca gcagggtgtg
     1021 agctgtttga agatactggg gttgggggtg aagaaactgc agaggactaa ctgggctgag
     1081 acccagtggc aatgttttag ggcctaagga atgcctctga aaatctagat ggacaacttt
     1141 gactttgaga aaagagaggt ggaaatgagg aaaatgactt ttctttatta gatttcggta
     1201 gaaagaactt tcatctttcc cctatttttg ttattcgttt taaaacatct atctggaggc
     1261 aggacaagta tggccattaa aaagatgcag gcagaaggca tatattggct cagtcaaagt
     1321 ggggaacttt ggtggccaaa catacattgc taaggctatt cctatatcag ctggacacat
     1381 ataaaatgct gctaatgctt cattacaaac ttatatcctt taattccaga tgggggcaaa
     1441 gtatgtccag gggtgaggaa caattgaaac atttgggctg gagtagattt tgaaagtcag
     1501 ctctgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgnncgc gcacgtgtgt ttgtgtgtgt
     1561 gtgagagcgt gtgtttcttt taacgttttc agcctacagc atacagggtt catggtggca
     1621 agaagataac aagatttaaa ttatggccag tgactagtgc tgcaagaaga acaactacct
     1681 gcatttaatg ggaaagcaaa atctcaggct ttgagggaag ttaacatagg cttgattctg
     1741 ggtggaagct gggtgtgtag ttatctggag gccaggctgg agctctcagc tcactatggg
     1801 ttcatcttta ttgtctcctt tcatctcaac aggctaagaa tcttctctgt ggcttttacg
     1861 gccgccatgc ggagctgcgc ttcttggacc tggttccttc tttgcagttg gacccggccc
     1921 agatctacag ggtcacttgg ttcatctcct ggagcccctg cttctcctgg ggctgtgccg
     1981 gggaagtgcg tgcgttcctt caggagaaca cacacgtgag actgcgcatc ttcgctgccc
     2041 gcatctatga ttacgacccc ctatataagg aggcgctgca aatgctgcgg gatgctgggg
     2101 cccaagtctc catcatgacc tacgatgaat ttaagcactg ctgggacacc tttgtggacc
     2161 accagggatg tcccttccag ccctgggatg gactagatga gcacagccaa gccctgagtg
     2221 ggaggctgcg ggccattctc cagaatcagg gaaacggaag cggcagcgag actcccggga
     2281 cctcagagtc cgccacaccc gaaagtgata aaaagtattc tattggttta gccatcggca
     2341 ctaattccgt tggatgggct gtcataaccg atgaatacaa agtaccttca aagaaattta
     2401 aggtgttggg gaacacagac cgtcattcga ttaaaaagaa tcttatcggt gccctcctat
     2461 tcgatagtgg cgaaacggca gaggcgactc gcctgaaacg aaccgctcgg agaaggtata
     2521 cacgtcgcaa gaaccgaata tgttacttac aagaaatttt tagcaatgag atggccaaag
     2581 ttgacgattc tttctttcac cgtttggaag agtccttcct tgtcgaagag gacaagaaac
     2641 atgaacggca ccccatcttt ggaaacatag tagatgaggt ggcatatcat gaaaagtacc
     2701 caacgattta tcacctcaga aaaaagctag ttgactcaac tgataaagcg gacctgaggt
     2761 taatctactt ggctcttgcc catatgataa agttccgtgg gcactttctc attgagggtg
     2821 atctaaatcc ggacaactcg gatgtcgaca aactgttcat ccagttagta caaacctata
     2881 atcagttgtt tgaagagaac cctataaatg caagtggcgt ggatgcgaag gctattctta
     2941 gcgcccgcct ctctaaatcc cgacggctag aaaacctgat cgcacaatta cccggagaga
     3001 agaaaaatgg gttgttcggt aaccttatag cgctctcact aggcctgaca ccaaatttta
     3061 agtcgaactt cgacttagct gaagatgcca aattgcagct tagtaaggac acgtacgatg
     3121 acgatctcga caatctactg gcacaaattg gagatcagta tgcggactta tttttggctg
     3181 ccaaaaacct tagcgatgca atcctcctat ctgacatact gagagttaat actgagatta
     3241 ccaaggcgcc gttatccgct tcaatgatca aaaggtacga tgaacatcac caagacttga
     3301 cacttctcaa ggccctagtc cgtcagcaac tgcctgagaa atataaggaa atattctttg
     3361 atcagtcgaa aaacgggtac gcaggttata ttgacggcgg agcgagtcaa gaggaattct
     3421 acaagtttat caaacccata ttagagaaga tggatgggac ggaagagttg cttgtaaaac
     3481 tcaatcgcga agatctactg cgaaagcagc ggactttcga caacggtagc attccacatc
     3541 aaatccactt aggcgaattg catgctatac ttagaaggca ggaggatttt tatccgttcc
     3601 tcaaagacaa tcgtgaaaag attgagaaaa tcctaacctt tcgcatacct tactatgtgg
     3661 gacccctggc ccgagggaac tctcggttcg catggatgac aagaaagtcc gaagaaacga
     3721 ttactccatg gaattttgag gaagttgtcg ataaaggtgc gtcagctcaa tcgttcatcg
     3781 agaggatgac caactttgac aagaatttac cgaacgaaaa agtattgcct aagcacagtt
     3841 tactttacga gtatttcaca gtgtacaatg aactcacgaa agttaagtat gtcactgagg
     3901 gcatgcgtaa acccgccttt ctaagcggag aacagaagaa agcaatagta gatctgttat
     3961 tcaagaccaa ccgcaaagtg acagttaagc aattgaaaga ggactacttt aagaaaattg
     4021 aatgcttcga ttctgtcgag atctccgggg tagaagatcg atttaatgcg tcacttggta
     4081 cgtatcatga cctcctaaag ataattaaag ataaggactt cctggataac gaagagaatg
     4141 aagatatctt agaagatata gtgttgactc ttaccctctt tgaagatcgg gaaatgattg
     4201 aggaaagact aaaaacatac gctcacctgt tcgacgataa ggttatgaaa cagttaaaga
     4261 ggcgtcgcta tacgggctgg ggacgattgt cgcggaaact tatcaacggg ataagagaca
     4321 agcaaagtgg taaaactatt ctcgattttc taaagagcga cggcttcgcc aataggaact
     4381 ttatgcagct gatccatgat gactctttaa ccttcaaaga ggatatacaa aaggcacagg
     4441 tttccggaca aggggactca ttgcacgaac atattgcgaa tcttgctggt tcgccagcca
     4501 tcaaaaaggg catactccag acagtcaaag tagtggatga gctagttaag gtcatgggac
     4561 gtcacaaacc ggaaaacatt gtaatcgaga tggcacgcga aaatcaaacg actcagaagg
     4621 ggcaaaaaaa cagtcgagag cggatgaaga gaatagaaga gggtattaaa gaactgggca
     4681 gccagatctt aaaggagcat cctgtggaaa atacccaatt gcagaacgag aaactttacc
     4741 tctattacct acaaaatgga agggacatgt atgttgatca ggaactggac ataaaccgtt
     4801 tatctgatta cgacgtcgat cacattgtac cccaatcctt tttgaaggac gattcaatcg
     4861 acaataaagt gcttacacgc tcggataaga accgagggaa aagtgacaat gttccaagcg
     4921 aggaagtcgt aaagaaaatg aagaactatt ggcggcagct cctaaatgcg aaactgataa
     4981 cgcaaagaaa gttcgataac ttaactaaag ctgagagggg tggcttgtct gaacttgaca
     5041 aggccggatt tattaaacgt cagctcgtgg aaacccgcca aatcacaaag catgttgcac
     5101 agatactaga ttcccgaatg aatacgaaat acgacgagaa cgataagctg attcgggaag
     5161 tcaaagtaat cactttaaag tcaaaattgg tgtcggactt cagaaaggat tttcaattct
     5221 ataaagttag ggagataaat aactaccacc atgcgcacga cgcttatctt aatgccgtcg
     5281 tagggaccgc actcattaag aaatacccga agctagaaag tgagtttgtg tatggtgatt
     5341 acaaagttta tgacgtccgt aagatgatcg cgaaaagcga acaggagata ggcaaggcta
     5401 cagccaaata cttcttttat tctaacatta tgaatttctt taagacggaa atcactctgg
     5461 caaacggaga gatacgcaaa cgacctttaa ttgaaaccaa tggggagaca ggtgaaatcg
     5521 tatgggataa gggccgggac ttcgcgacgg tgagaaaagt tttgtccatg ccccaagtca
     5581 acatagtaaa gaaaactgag gtgcagaccg gagggttttc aaaggaatcg attcttccaa
     5641 aaaggaatag tgataagctc atcgctcgta aaaaggactg ggacccgaaa aagtacggtg
     5701 gcttcgatag ccctacagtt gcctattctg tcctagtagt ggcaaaagtt gagaagggaa
     5761 aatccaagaa actgaagtca gtcaaagaat tattggggat aacgattatg gagcgctcgt
     5821 cttttgaaaa gaaccccatc gacttccttg aggcgaaagg ttacaaggaa gtaaaaaagg
     5881 atctcataat taaactacca aagtatagtc tgtttgagtt agaaaatggc cgaaaacgga
     5941 tgttggctag cgccggagag cttcaaaagg ggaacgaact cgcactaccg tctaaatacg
     6001 tgaatttcct gtatttagcg tcccattacg agaagttgaa aggttcacct gaagataacg
     6061 aacagaagca actttttgtt gagcagcaca aacattatct cgacgaaatc atagagcaaa
     6121 tttcggaatt cagtaagaga gtcatcctag ctgatgccaa tctggacaaa gtattaagcg
     6181 catacaacaa gcacagggat aaacccatac gtgagcaggc ggaaaatatt atccatttgt
     6241 ttactcttac caacctcggc gctccagccg cattcaagta ttttgacaca acgatagatc
     6301 gcaaacgata cacttctacc aaggaggtgc tagacgcgac actgattcac caatccatca
     6361 cgggattata tgaaactcgg atagatttgt cacagcttgg gggtgactct ggtggttcta
     6421 ctaatctgtc agatattatt gaaaaggaga ccggtaagca actggttatc caggaatcca
     6481 tcctcatgct cccagaggag gtggaagaag tcattgggaa caagccggaa agcgatatac
     6541 tcgtgcacac cgcctacgac gagagcaccg acgagaatgt catgcttctg actagcgacg
     6601 cccctgaata caagccttgg gctctggtca tacaggatag caacggtgag aacaagatta
     6661 agatgctctc tggtggttct cccaagaaga agaggaaagt ctaaccggtc atcatcacca
     6721 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6781 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6841 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6901 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6961 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc
     7021 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7081 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctagggtgc
     7141 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7201 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7261 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7321 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7381 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7441 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7501 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7561 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7621 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7681 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7741 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7801 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7861 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7921 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7981 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8041 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8101 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8161 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8221 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8281 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8341 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8401 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8461 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8521 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8581 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8641 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8701 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8761 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8821 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8881 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8941 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9001 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9061 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9121 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9181 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     9241 agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9301 tgcttgtgtg ttggaggtcg ctgag
//
