LOCUS       sequence-212953-        5143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene cloning of human CD1E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Facciotti F, Cavallari M, Angenieux C, Garcia-Alles LF, 
            Signorino-Gelo F, Angman L, Gilleron M, Prandi J, Puzo G, Panza L, 
            Xia C, Wang PG, Dellabona P, Casorati G, Porcelli SA, de la Salle H,
            Mori L, De Libero G
  TITLE     Fine tuning by human CD1e of lipid-specific immune responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14228-33. doi: 
            10.1073/pnas.1108809108. Epub 2011 Aug 15.
  PUBMED    21844346
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1108809108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-08-23- 23"; volume: "108"; issue: "34"; pages: 
            "14228-33"
COMMENT     Sequence Label: pHCD1E
FEATURES             Location/Qualifiers
     source          1..5143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(629..1420,1421..1489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1252..1271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1507..2301)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1618..1637)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2228..2247
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(2645..3073)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2755..2776)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2967..2986
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3199..3221
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3213..3230
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3214..3230
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3237..3256
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3237..3255
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3912..3923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4611..4627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4611..4627)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4624..4646)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4635..4651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4659..4665,4666..4683,4684..4689))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4704..4725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4842..4859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5013..5143,1..458))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5093..5112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
       61 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1501 ttcaattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     1561 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     1621 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     1681 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     1741 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     1801 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     1861 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     1921 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     1981 aaggtgggat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     2041 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     2101 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     2161 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     2221 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     2281 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga
     2341 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     2401 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     2461 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     2521 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     2581 ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgaattttg
     2641 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2701 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2761 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2821 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     2881 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2941 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3001 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3061 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     3121 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     3181 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     3241 acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc cgccagtgtg
     3301 atggatatct gcagaattcg cccttctcga gtttaacaga gcttcaatgc tgctcctgtt
     3361 cctcctcttc gagggtctct gctgtcctgg ggaaaataca gcagctcccc aggctctaca
     3421 atcctatcat ctagcagcag aggagcagct gtccttccgc atgctccaaa cttcctcctt
     3481 tgccaaccac agctgggcac acagtgaggg ctcaggatgg ctgggtgacc tgcagactca
     3541 tggctgggac actgtcttgg gcaccatccg ctttctgaag ccctggtccc atggaaactt
     3601 cagcaagcag gagctgaaaa acttacagtc actgttccag ttatacttcc atagttttat
     3661 ccggatagtg caagcttctg ctggtcaatt tcagcttgaa taccccttcg agatccagat
     3721 attagctggc tgtagaatga atgccccaca aatcttctta aatatggcat atcaagggtc
     3781 agatttcctg agtttccaag gaatttcctg ggagccatct ccaggagcag ggatccgggc
     3841 ccagaacatc tgtaaagtgc tcaatcgcta cctagatatt aaggaaatac tgcaaagcct
     3901 tcttggtcac acctgccctc gatttctagc ggggctcatg gaagcagggg agtcagaact
     3961 gaaacggaaa gtgaagccag aggcctggct gtcctgtggc cccagtcctg gccctggccg
     4021 tctgcagctt gtgtgccatg tctcaggatt ctacccaaag cccgtgtggg tgatgtggat
     4081 gcggggtgag caggagcagc ggggcactca gcgaggggac gtcctgccta atgctgacga
     4141 gacatggtat ctccgagcaa ccctggatgt ggcggctggg gaggcagctg gcctgtcctg
     4201 tcgggtgaaa cacagcagtc tagggggcca tgatctaatc atccattggg gtggatattc
     4261 catctttctc atcctgatct gtttgactgt gatagttacc ctggtcatat tggttgtagt
     4321 tgactcacgg ttaaaaaaac agagttcaaa taagaacatt ctttctcccc acacacccag
     4381 ccctgtcttt ctcatgggag ccaacactca ggacaccaag aattcaagac atcagttctg
     4441 cttggcacaa gtatcgtgga tcaaaaacag agtattgaag aagtggaaga cacgcctaaa
     4501 ccaactctgg tgacatttgc tttaccttag cggccgcaag ggcgaattcc agcacactgg
     4561 cggccgttac tagtggatcc gagctcggta ccaagcttgg cgtaatcatg gtcatagctg
     4621 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4681 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4741 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4801 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4861 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttc
//
