LOCUS       sequence-212954-       14894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in
            transgenic mice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Hohenstein P, Slight J, Ozdemir DD, Burn SF, Berry R, Hastie ND
  TITLE     High-efficiency Rosa26 knock-in vector construction for 
            Cre-regulated overexpression and RNAi.
  JOURNAL   Pathogenetics. 2008  . 1(1):3.
  PUBMED    19014667
REFERENCE   2  (bases 1 to 14894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Pathogenetics. 2008  . 1(1):3."
COMMENT     Sequence Label: pRosa26-DEST
FEATURES             Location/Qualifiers
     source          1..14894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..1088
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(1107..1172,1173..1210)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     1243..1259
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1243..1259
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    1273..1306
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1336..1835
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1849..2652
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1912..1931)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2522..2541
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2662..3123
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    3271..3405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3525..3659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3779..3913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3942..3975
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4031..4155
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4180..4185,4186..4203,4204..4210)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4264..4944
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(4330..4349)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5264..5569
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5448..5467
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5610..5734)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    5781..6005
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6059..10399
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        10449..10948
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             10975..11547
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          11624..11689
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    11763..11987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12049..12069)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12049..12067)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12088..12104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12088..12104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12101..12123)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12112..12128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12136..12142,12143..12160,12161..12166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12181..12202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12319..12336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12490..13078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12570..12589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13249..14040,14041..14109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13872..13891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14110..14214)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14267..14695)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14377..14398)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14589..14608
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14822..14844
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14836..14853
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14837..14853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14863..14882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14863..14881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccgcggc aggccctccg agcgtggtgg agccgttctg tgagacagcc gggtacgagt
       61 cgtgacgctg gaaggggcaa gcgggtggtg ggcaggaatg cggtccgccc tgcagcaacc
      121 ggagggggag ggagaaggga gcggaaaagt ctccaccgga cgcggccatg gctcgggggg
      181 gggggggcag cggaggagcg cttccggccg acgtctcgtc gctgattggc ttcttttcct
      241 cccgccgtgt gtgaaaacac aaatggcgtg ttttggttgg cgtaaggcgc ctgtcagtta
      301 acggcagccg gagtgcgcag ccgccggcag cctcgctctg cccactgggt ggggcgggag
      361 gtaggtgggg tgaggcgagc tggacgtgcg ggcgcggtcg gcctctggcg gggcgggggg
      421 ggggagggag ggtcagcgaa agtagctcgc gcgcgagcgg ccgcccaccc tccccttcct
      481 ctgggggagt cgttttaccc gccgccggcc gggcctcgtc gtctgattgg ctctcggggc
      541 ccagaaaact ggcccttgcc attggctcgt gttcgtgcaa gttgagtcca tccgccggcc
      601 agcgggggcg gcgaggaggc gctcccaggt tccggccctc ccctcggccc cgcgccgcag
      661 agtctggccg cgcgcccctg cgcaacgtgg caggaagcgc gcgctggggg cggggacggg
      721 cagtagggct gagcggctgc ggggcgggtg caagcacgtt tccgacttga gttgcctcaa
      781 gaggggcgtg ctgagccaga cctccatcgc gcactccggg gagtggaggg aaggagcgag
      841 ggctcagttg ggctgttttg gaggcaggaa gcacttgctc tcccaaagtc gctctgagtt
      901 gttatcagta agggagctgc agtggagtag gcggggagaa ggccgcaccc ttctccggag
      961 gggggagggg agtgttgcaa tacctttctg ggagttctct gctgcctcct ggcttctgag
     1021 gaccgccctg ggcctgggag aatcccttcc ccctcttccc tcgtgatctg caactccagt
     1081 ctttctagcc ttaattaagg gatctgtagg gcgcagtagt ccagggtttc cttgatgatg
     1141 tcatacttat cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg
     1201 tctttccagt ggggatcgac ggtatcgtag agtcgaggcc gctctagaac tagtggatcc
     1261 ggaaccctta atataacttc gtataatgta tgctatacga agttattagg tccctcgacc
     1321 tgcaggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     1381 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     1441 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     1501 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     1561 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     1621 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     1681 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     1741 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     1801 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaatat gggatcggcc
     1861 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     1921 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     1981 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     2041 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     2101 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     2161 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     2221 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     2281 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     2341 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     2401 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     2461 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     2521 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     2581 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     2641 gagttcttct gaggggatcc gctgtaagtc tgcagaaatt gatgatctat taaacaataa
     2701 agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg tgagaacaga
     2761 gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg tgggattaga
     2821 taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg tttcatagtt
     2881 ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc tcccactcat
     2941 gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt cccactttgt
     3001 ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa cgagatcagc
     3061 agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct aattccatca
     3121 gaagcttgca gatctgcgac tctagaggat ctgcgactct agaggatcat aatcagccat
     3181 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     3241 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     3301 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3361 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgcga ctctagagga
     3421 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     3481 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     3541 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3601 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     3661 gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3721 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3781 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3841 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3901 tcatgtctgg atccccatca agctgatccg gaacccttaa tataacttcg tataatgtat
     3961 gctatacgaa gttattaggt ccctcgacct gcagcccaag ctagcttatc gataccgtcg
     4021 acctcatcaa acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     4081 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     4141 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     4201 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     4261 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     4321 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     4381 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     4441 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     4501 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     4561 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     4621 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     4681 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     4741 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     4801 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     4861 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca ggcggggcgt
     4921 aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     4981 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     5041 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     5101 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     5161 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     5221 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     5281 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     5341 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     5401 cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     5461 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     5521 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     5581 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     5641 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     5701 acgtttctcg ttcagctttc ttgtacaaag tggttcgatg ggcccccgcg ggcggccgcg
     5761 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5821 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5881 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5941 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6001 tatggcttct gaggcggaaa gaaccagctg gggctcgatc ctctagttgg cgcgccggct
     6061 agaagatggg cgggagtctt ctgggcaggc ttaaaggcta acctggtgtg tgggcgttgt
     6121 cctgcagggg aattgaacag gtgtaaaatt ggagggacaa gacttcccac agattttcgg
     6181 ttttgtcggg aagtttttta ataggggcaa ataaggaaaa tgggaggata ggtagtcatc
     6241 tggggtttta tgcagcaaaa ctacaggtta ttattgcttg tgatccgcct cggagtattt
     6301 tccatcgagg tagattaaag acatgctcac ccgagtttta tactctcctg cttgagatcc
     6361 ttactacagt atgaaattac agtgtcgcga gttagactat gtaagcagaa ttttaatcat
     6421 ttttaaagag cccagtactt catatccatt tctcccgctc cttctgcagc cttatcaaaa
     6481 ggtattttag aacactcatt ttagccccat tttcatttat tatactggct tatccaaccc
     6541 ctagacagag cattggcatt ttccctttcc tgatcttaga agtctgatga ctcatgaaac
     6601 cagacagatt agttacatac accacaaatc gaggctgtag ctggggcctc aacactgcag
     6661 ttcttttata actccttagt acactttttg ttgatccttt gccttgatcc ttaattttca
     6721 gtgtctatca cctctcccgt caggtggtgt tccacatttg ggcctattct cagtccaggg
     6781 agttttacaa caatagatgt attgagaatc caacctaaag cttaactttc cactcccatg
     6841 aatgcctctc tcctttttct ccatttataa actgagctat taaccattaa tggtttccag
     6901 gtggatgtct cctcccccaa tattacctga tgtatcttac atattgccag gctgatattt
     6961 taagacatta aaaggtatat ttcattattg agccacatgg tattgattac tgcttactaa
     7021 aattttgtca ttgtacacat ctgtaaaagg tggttccttt tggaatgcaa agttcaggtg
     7081 tttgttgtct ttcctgacct aaggtcttgt gagcttgtat tttttctatt taagcagtgc
     7141 tttctcttgg actggcttga ctcatggcat tctacacgtt attgctggtc taaatgtgat
     7201 tttgccaagc ttcttcagga cctataattt tgcttgactt gtagccaaac acaagtaaaa
     7261 tgattaagca acaaatgtat ttgtgaagct tggtttttag gttgttgtgt tgtgtgtgct
     7321 tgtgctctat aataatacta tccaggggct ggagaggtgg ctcggagttc aagagcacag
     7381 actgctcttc cagaagtcct gagttcaatt cccagcaacc acatggtggc tcacaaccat
     7441 ctgtaatggg atctgatgcc ctcttctggt gtgtctgaag accacaagtg tattcacatt
     7501 aaataaataa atcctccttc ttcttctttt tttttttttt aaagagaata ctgtctccag
     7561 tagaatttac tgaagtaatg aaatactttg tgtttgttcc aatatggtag ccaataatca
     7621 aattactctt taagcactgg aaatgttacc aaggaactaa tttttatttg aagtgtaact
     7681 gtggacagag gagccataac tgcagacttg tgggatacag aagaccaatg cagactttaa
     7741 tgtcttttct cttacactaa gcaataaaga aataaaaatt gaacttctag tatcctattt
     7801 gtttaaactg ctagctttac ttaacttttg tgcttcatct atacaaagct gaaagctaag
     7861 tctgcagcca ttactaaaca tgaaagcaag taatgataat tttggatttc aaaaatgtag
     7921 ggccagagtt tagccagcca gtggtggtgc ttgcctttat gcctttaatc ccagcactct
     7981 ggaggcagag acaggcagat ctctgagttt gagcccagcc tggtctacac atcaagttct
     8041 atctaggata gccaggaata cacacagaaa ccctgttggg gaggggggct ctgagatttc
     8101 ataaaattat aattgaagca ttccctaatg agccactatg gatgtggcta aatccgtcta
     8161 cctttctgat gagatttggg tattattttt tctgtctctg ctgttggttg ggtcttttga
     8221 cactgtgggc tttctttaaa gcctccttcc tgccatgtgg tctcttgttt gctactaact
     8281 tcccatggct taaatggcat ggctttttgc cttctaaggg cagctgctga gatttgcagc
     8341 ctgatttcca gggtggggtt gggaaatctt tcaaacacta aaattgtcct ttaatttttt
     8401 ttttaaaaaa tgggttatat aataaacctc ataaaatagt tatgaggagt gaggtggact
     8461 aatattaaat gagtccctcc cctataaaag agctattaag gctttttgtc ttatacttaa
     8521 cttttttttt aaatgtggta tctttagaac caagggtctt agagttttag tatacagaaa
     8581 ctgttgcatc gcttaatcag attttctagt ttcaaatcca gagaatccaa attcttcaca
     8641 gccaaagtca aattaagaat ttctgacttt taatgttaat ttgcttactg tgaatataaa
     8701 aatgatagct tttcctgagg cagggtctca ctatgtatct ctgcctgatc tgcaacaaga
     8761 tatgtagact aaagttctgc ctgcttttgt ctcctgaata ctaaggttaa aatgtagtaa
     8821 tacttttgga acttgcaggt cagattcttt tataggggac acactaaggg agcttgggtg
     8881 atagttggta aaatgtgttt caagtgatga aaacttgaat tattatcacc gcaacctact
     8941 ttttaaaaaa aaaagccagg cctgttagag catgcttaag ggatccctag gacttgctga
     9001 gcacacaaga gtagttactt ggcaggctcc tggtgagagc atatttcaaa aaacaaggca
     9061 gacaaccaag aaactacagt taaggttacc tgtctttaaa ccatctgcat atacacaggg
     9121 atattaaaat attccaaata atatttcatt caagttttcc cccatcaaat tgggacatgg
     9181 atttctccgg tgaataggca gagttggaaa ctaaacaaat gttggttttg tgatttgtga
     9241 aattgttttc aagtgatagt taaagcccat gagatacaga acaaagctgc tatttcgagg
     9301 tctcttggtt tatactcaga agcacttctt tgggtttccc tgcactatcc tgatcatgtg
     9361 ctaggcctac cttaggctga ttgttgttca aataaactta agtttcctgt caggtgatgt
     9421 catatgattt catatatcaa ggcaaaacat gttatatatg ttaaacattt gtacttaatg
     9481 tgaaagttag gtctttgtgg gtttgatttt taattttcaa aacctgagct aaataagtca
     9541 tttttacatg tcttacattt ggtggaattg tataattgtg gtttgcaggc aagactctct
     9601 gacctagtaa ccctacctat agagcacttt gctgggtcac aagtctagga gtcaagcatt
     9661 tcaccttgaa gttgagacgt tttgttagtg tatactagtt tatatgttgg aggacatgtt
     9721 tatccagaag atattcagga ctatttttga ctgggctaag gaattgattc tgattagcac
     9781 tgttagtgag cattgagtgg cctttaggct tgaattggag tcacttgtat atctcaaata
     9841 atgctggcct tttttaaaaa gcccttgttc tttatcaccc tgttttctac ataatttttg
     9901 ttcaaagaaa tacttgtttg gatctccttt tgacaacaat agcatgtttt caagccatat
     9961 tttttttcct tttttttttt ttttttggtt tttcgagaca gggtttctct gtatagccct
    10021 ggctgtcctg gaactcactt tgtagaccag gctggcctcg aactcagaaa tccgcctgcc
    10081 tctgcctcct gagtgccggg attaaaggcg tgcaccacca cgcctggcta agttggatat
    10141 tttgttatat aactataacc aatactaact ccactgggtg gatttttaat tcagtcagta
    10201 gtcttaagtg gtctttattg gcccttcatt aaaatctact gttcactcta acagaggctg
    10261 ttggtactag tggcacttaa gcaacttcct acggatatac tagcagatta agggtcaggg
    10321 atagaaacta gtctagcgtt ttgtatacct accagcttta tactaccttg ttctgataga
    10381 aatatttcag gacatctagc ttatcgatac cgtcgacggt atcgataagc ttgatatcga
    10441 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
    10501 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
    10561 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
    10621 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
    10681 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
    10741 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
    10801 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
    10861 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
    10921 ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc tcgccatgga tcctgatgat
    10981 gttgttgatt cttctaaatc ttttgtgatg gaaaactttt cttcgtacca cgggactaaa
    11041 cctggttatg tagattccat tcaaaaaggt atacaaaagc caaaatctgg tacacaagga
    11101 aattatgacg atgattggaa agggttttat agtaccgaca ataaatacga cgctgcggga
    11161 tactctgtag ataatgaaaa cccgctctct ggaaaagctg gaggcgtggt caaagtgacg
    11221 tatccaggac tgacgaaggt tctcgcacta aaagtggata atgccgaaac tattaagaaa
    11281 gagttaggtt taagtctcac tgaaccgttg atggagcaag tcggaacgga agagtttatc
    11341 aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc ttcccttcgc tgaggggagt
    11401 tctagcgttg aatatattaa taactgggaa caggcgaaag cgttaagcgt agaacttgag
    11461 attaattttg aaacccgtgg aaaacgtggc caagatgcga tgtatgagta tatggctcaa
    11521 gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag gaaccttact tctgtggtgt
    11581 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    11641 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    11701 tggaactgat gaatgggagc agtggtggaa tgcagatcct agagctcgct gatcagcctc
    11761 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    11821 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    11881 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    11941 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    12001 aagaaccagc tggggctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
    12061 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    12121 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    12181 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    12241 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    12301 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    12361 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    12421 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    12481 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12541 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12601 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12661 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12721 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12781 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12841 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    12901 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    12961 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13021 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    13141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    13201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    13261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    13321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    13381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    13441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    13501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    13561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    13621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    13681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    13741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    13801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    13861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    13921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    13981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    14041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    14101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    14161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    14221 tccccgaaaa gtgcaaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
    14281 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
    14341 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
    14401 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
    14461 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    14521 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    14581 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
    14641 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg caatttccat
    14701 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    14761 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    14821 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
    14881 ggcgaattgg agct
//
