LOCUS       sequence-212974-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PCDH7 transcript variant b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Zhou X, Updegraff BL, Guo Y, Peyton M, Girard L, Larsen JE, Xie XJ, 
            Zhou Y, Hwang TH, Xie Y, Rodriguez-Canales J, Villalobos P, Behrens 
            C, Wistuba II, Minna JD, O'Donnell KA
  TITLE     PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK 
            Signaling by EGFR and KRAS during Lung Tumorigenesis.
  JOURNAL   Cancer Res. 2017 Jan 1;77(1):187-197. doi: 
            10.1158/0008-5472.CAN-16-1267-T. Epub 2016 Nov 7.
  PUBMED    27821484
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-16-1267-T"; journalName: "Cancer Res"; date: 
            "2017-01-1- 1"; volume: "77"; issue: "1"; pages: "187-197"
COMMENT     Sequence Label: pLX304-PCDH7-b
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     522..542
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    594..618
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3869..3893)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3895..3936
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3978..4566
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4031..4051)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4449..4460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4638..4871
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4949..5070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4986..5005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5040..5059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5110..5245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5265..5284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5266..5284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5294..5311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5294..5310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5303..5325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5452..5907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5539..5558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5749..5770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7069..7657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7558..7577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7811..7828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7945..7966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7981..7986,7987..8004,8005..8011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8019..8035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8024..8046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8043..8059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8043..8059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8078..8098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8080..8098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8126..8352
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8353..8533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8580..8705
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9198..9431
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9616..9660
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9835..10335
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     10254..10272
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             10347..10742
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10802..10919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccggggttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      421 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      481 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      541 tgtacggtgg gaggtctata taagcagagc tctctggcta actgtcggga tcaacaagtt
      601 tgtacaaaaa agcaggctcc gaattcgccc ttcaccatgc tgaggatgcg gaccgcggga
      661 tgggcgcgcg gctggtgctt gggctgctgc ctcctcctgc cgctctcgct cagcctggcg
      721 gccgccaagc agctcctccg gtaccggctg gccgaggagg gccccgccga cgtccgcatc
      781 ggcaacgtgg cttcagacct gggcatcgtg accggatcgg gtgaggtgac tttcagcctg
      841 gagtccggtt ccgagtacct gaagatcgac aacctcactg gcgagctgag cacgagcgag
      901 cggcgcatcg accgcgagaa gctgccccag tgtcagatga tcttcgacga gaacgagtgc
      961 ttcctggact tcgaggtgtc ggtgatcggg ccctcgcaga gctgggtgga cctgtttgag
     1021 ggtcaggtca tcgtgcttga catcaacgac aacacgccca ccttcccgtc gcccgtgctc
     1081 acgctcacgg tggaggagaa tcggccggtg ggcacacttt acctgctgcc cacagccacc
     1141 gaccgcgact tcggccgcaa cggcatcgag cgctacgagc tgctccagga gcccggaggc
     1201 ggcggcagcg gcggcgagag ccggcgcgcc ggggcggccg acagcgcccc ctaccccggg
     1261 ggcggcggga acggcgcgag cggcggcggc tcgggaggct ccaagcggcg gctggacgca
     1321 tcagagggcg gcggcggcac caaccccggc ggccgcagca gcgtgttcga gctgcaggtg
     1381 gcggacaccc cggacggcga gaagcagccg cagctgatcg tgaagggggc gctggaccgc
     1441 gagcagcgcg actcctacga gctgaccctg cgagtgcgcg acggcggcga cccgcctcgc
     1501 tcctcgcagg ccatcctacg ggtcctcatc accgacgtga acgacaacag cccccgcttc
     1561 gagaagagcg tgtacgaggc cgacttggct gagaacagcg ccccggggac ccccatcctg
     1621 caactgcgcg cagccgactt ggacgtgggg gtcaacgggc agatcgaata cgtgttcggg
     1681 gcggccaccg agtcggtgag gcggctgctg cgccttgacg agacgtccgg ctggctcagc
     1741 gtcctgcacc ggatcgaccg cgaggaggtg aaccagctgc gcttcacggt catggcccgc
     1801 gaccgcgggc agccccccaa gaccgacaag gccaccgtgg tccttaacat caaagacgag
     1861 aacgacaacg tgccgtccat tgaaatccgc aagattgggc gcatccccct caaggacggg
     1921 gtggccaacg tggccgagga cgttctggtc gacaccccca tcgctctggt gcaggtgtcc
     1981 gaccgagacc aaggcgagaa cggggtggtc acctgcaccg tggtgggcga cgtgcccttc
     2041 cagctcaagc cagccagcga caccgagggc gaccagaaca agaaaaagta cttcttgcac
     2101 acctcgaccc ctctggacta tgaggccacc cgggagttca acgtggtcat cgtggcggtg
     2161 gactcaggca gccccagcct ctcgagcaac aactccctga ttgtcaaggt gggagacacc
     2221 aacgacaacc cgcccatgtt cggccagtcg gtggtggagg tttacttccc tgagaacaac
     2281 atcccgggcg agagggtggc cacggtgctg gcgacagacg cagacagcgg taagaacgcc
     2341 gagatcgcct actcgctgga ctcctctgtg atggggatct ttgccatcga tcccgattct
     2401 ggggacatcc tggtcaatac cgtgctggac cgcgagcaga ctgacaggta tgagtttaaa
     2461 gttaacgcca aagacaaagg catccccgtg ctgcagggca gcactacggt gattgtgcag
     2521 gtggctgata aaaatgacaa tgaccctaag tttatgcagg acgtcttcac cttttatgtg
     2581 aaagaaaact tgcagcccaa cagccctgtg gggatggtca ccgtgatgga tgctgacaag
     2641 gggcggaatg cagagatgag cctgtacata gaggagaaca ataacatttt ttctattgaa
     2701 aatgacacgg ggaccattta ctccacaatg tcttttgacc gggaacatca gaccacatac
     2761 actttcagag tcaaggctgt ggatggggga gatcctccca gatctgccac agctacagtc
     2821 tcgctttttg tgatggatga aaatgacaat gctcccacag ttacccttcc caaaaacatt
     2881 tcctacactt tactgccacc ttcgagtaat gtcaggacag tagtagctac agtgttggca
     2941 acagacagtg atgatggcat caatgcagac ctgaactaca gcattgtggg aggaaatccc
     3001 ttcaagctgt ttgaaattga tcccactagt ggtgtggttt ccttagtggg aaaactcacc
     3061 caaaagcatt atggcttgca caggttggtg gtgcaagtga atgacagtgg gcagccttcc
     3121 cagtccacca cgactctggt gcacgtgttt gtcaatgaaa gtgtttctaa tgcaactgcg
     3181 attgactccc agatagctag aagtttgcac atcccactca cccaggatat agctggtgac
     3241 ccaagctatg aaattagcaa acagagactc agtattgtca ttggcgtggt tgctggcatt
     3301 atgacggtga ttctaatcat cttaattgta gtgatggcaa ggtactgcag gtccaaaaat
     3361 aaaaatggct atgaagccgg caaaaaagat cacgaagact tttttacacc ccaacagcat
     3421 gacaaatcta aaaagcctaa aaaggacaag aaaaacaaaa aatctaagca gcctctctac
     3481 agcagcattg tcactgtgga ggcttctaag ccaaatggac agaggtatga tagtgtcaat
     3541 gagaagctgt cagacagccc aagcatgggg cgatacaggt ccgttaatgg tgggcccggc
     3601 agtcctgacc tggcaaggca ttacaaatct agttccccat tgcctactgt tcagcttcat
     3661 ccccagtcac caactgcagg aaaaaaacac caggccgtac aagatctacc accagccaac
     3721 acatttgtgg gagcaggaga caacatttca attggatcag atcactgctc tgagtacagc
     3781 tgtcaaacca ataacaagta cagcaaacag gtaagatgta tcccaaatat atttaaatat
     3841 cccagggagg gctaaaaggg cgaattcgac ccagctttct tgtacaaagt ggttggtaag
     3901 cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg
     3961 tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4021 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4081 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4141 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4201 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4261 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4381 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4441 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4501 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4561 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4621 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     4681 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4741 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4801 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4861 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     4921 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     4981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5221 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     5281 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     5461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5521 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5641 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5761 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5821 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5881 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7741 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7801 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7861 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7921 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7981 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8041 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     8101 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     8161 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     8221 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     8281 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     8341 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8521 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8581 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8641 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8701 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8761 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8821 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8881 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8941 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     9001 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     9061 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     9121 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     9181 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9241 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     9301 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9361 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9421 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9481 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9541 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9601 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9661 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9721 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9781 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
     9841 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     9901 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     9961 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
    10021 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
    10081 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
    10141 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
    10201 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
    10261 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
    10321 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
    10381 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
    10441 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
    10501 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
    10561 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
    10621 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
    10681 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
    10741 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
    10801 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    10861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    10921 gggtttatta cagggacagc agagatccac tttggcgccg gctcgagggg gc
//
