LOCUS       sequence-212980-       11587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector carrying human EF1a promoter-driven 
            dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Chong ZS, Ohnishi S, Yusa K, Wright GJ
  TITLE     Pooled extracellular receptor-ligand interaction screening using 
            CRISPR activation.
  JOURNAL   Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
  PUBMED    30477585
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-11-26"; volume: "19(1):205. doi"; pages: " 
            10.1186/s13059-018-1581-3"
COMMENT     Sequence Label: pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
FEATURES             Location/Qualifiers
     source          1..11587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        908..2093
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1139..2085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2042..2062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2125..2172
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(2173..2196,2197..2199,2200..4755,4756..4758,
                     4759..6273)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6328..6348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6373..6522
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6553..6606
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6613..6622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6622..7008
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             7063..7083
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7099..7641
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7666..8037
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             8075..8095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(8120..8693,8694..8696,8697..8705)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8287..8304)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8514..8533
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8706..9509
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8769..8788)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9379..9398
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9541..9558)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9547..9771
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(9816..9915)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10027..10046)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10038..10847
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10105..10124)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10937..11525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11426..11445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccac gcgtgagggg cgcggaagcc ggggcggagg aggagccaac cccgggctgg
      541 gctgagaccc gcagaggaag acgctctagg gatttgtccc ggactagcga gatggcaagg
      601 ctgaggacgg gaggctgatt gagaggcgaa ggtacaccct aatctcaata caacctttgg
      661 agctaagcca gcaatggtag agggaagatt ctgcacgtcc cttccaggcg gcctccccgt
      721 caccaccccc cccaacccgc cccgaccgga gctgagagta attcatacaa aaggactcgc
      781 ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga
      841 tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca
      901 tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      961 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     1021 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1081 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     1141 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     1201 ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     1261 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     1321 ttgaggcctg gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     1381 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     1441 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tcgatctgca cactggtatt
     1501 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1561 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     1621 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     1681 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     1741 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1801 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1861 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1921 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1981 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     2041 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgggaagct
     2101 tggcgcgccc gtacggccac catgaaaagg ccggcggcca cgaaaaaggc cggccaggca
     2161 aaaaagaaaa aggacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     2221 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     2281 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     2341 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     2401 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     2461 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     2521 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     2581 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     2641 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     2701 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     2761 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     2821 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     2881 ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     2941 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     3001 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     3061 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     3121 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     3181 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     3241 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     3301 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     3361 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     3421 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     3481 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     3541 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     3601 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     3661 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     3721 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     3781 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     3841 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     3901 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     3961 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     4021 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     4081 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     4141 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     4201 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     4261 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     4321 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     4381 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     4441 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     4501 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     4561 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     4621 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     4681 gtggaccaca tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     4741 accagaagcg acaaggcccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     4801 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     4861 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     4921 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     4981 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     5041 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     5101 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     5161 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     5221 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     5281 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     5341 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     5401 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     5461 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     5521 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     5581 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     5641 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     5701 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     5761 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     5821 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     5881 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     5941 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     6001 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     6061 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     6121 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     6181 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     6241 acacggatcg acctgtctca gctgggaggc gacagcgctg gaggaggtgg aagcggagga
     6301 ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctgga
     6361 tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc
     6421 gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg
     6481 ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaactg tacgggcggc
     6541 gggtccggag gagagggcag aggaagtctc ctaacatgcg gtgacgtgga ggagaatcct
     6601 ggcccatgta cggccaccat ggcttcaaac tttactcagt tcgtgctcgt ggacaatggt
     6661 gggacagggg atgtgacagt ggctccttct aatttcgcta atggggtggc agagtggatc
     6721 agctccaact cacggagcca ggcctacaag gtgacatgca gcgtcaggca gtctagtgcc
     6781 cagaagagaa agtataccat caaggtggag gtccccaaag tggctaccca gacagtgggc
     6841 ggagtcgaac tgcctgtcgc cgcttggagg tcctacctga acatggagct cactatccca
     6901 attttcgcta ccaattctga ctgtgaactc atcgtgaagg caatgcaggg gctcctcaaa
     6961 gacggtaatc ctatcccttc cgccatcgcc gctaactcag gtatctacag cgctggagga
     7021 ggtggaagcg gaggaggagg aagcggagga ggaggtagcg gacctaagaa aaagaggaag
     7081 gtggcggccg ctggatcccc ttcagggcag atcagcaacc aggccctggc tctggcccct
     7141 agctccgctc cagtgctggc ccagactatg gtgccctcta gtgctatggt gcctctggcc
     7201 cagccacctg ctccagcccc tgtgctgacc ccaggaccac cccagtcact gagcgctcca
     7261 gtgcccaagt ctacacaggc cggcgagggg actctgagtg aagctctgct gcacctgcag
     7321 ttcgacgctg atgaggacct gggagctctg ctggggaaca gcaccgatcc cggagtgttc
     7381 acagatctgg cctccgtgga caactctgag tttcagcagc tgctgaatca gggcgtgtcc
     7441 atgtctcata gtacagccga accaatgctg atggagtacc ccgaagccat tacccggctg
     7501 gtgaccggca gccagcggcc ccccgacccc gctccaactc ccctgggaac cagcggcctg
     7561 cctaatgggc tgtccggaga tgaagacttc tcaagcatcg ctgatatgga ctttagtgcc
     7621 ctgctgtcac agatttcctc tagtgggcag ggaggaggtg gaagcggctt cagcgtggac
     7681 accagtgccc tgctggacct gttcagcccc tcggtgaccg tgcccgacat gagcctgcct
     7741 gaccttgaca gcagcctggc cagtatccaa gagctcctgt ctccccagga gccccccagg
     7801 cctcccgagg cagagaacag cagcccggat tcagggaagc agctggtgca ctacacagcg
     7861 cagccgctgt tcctgctgga ccccggctcc gtggacaccg ggagcaacga cctgccggtg
     7921 ctgtttgagc tgggagaggg ctcctacttc tccgaagggg acggcttcgc cgaggacccc
     7981 accatctccc tgctgacagg ctcggagcct cccaaagcca aggaccccac tgtctcctgt
     8041 acatgagaat tcgcggccgc aaggtggagg tggacccaag aagaagcgca aggtgtgagt
     8101 taacggccgc cgcccccccc ccctctccct cccccccccc taacgttact ggccgaagcc
     8161 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     8221 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     8281 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     8341 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     8401 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     8461 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     8521 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     8581 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct
     8641 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     8701 caaccatggg atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8761 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     8821 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     8881 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8941 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     9001 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     9061 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     9121 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     9181 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     9241 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     9301 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     9361 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9421 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9481 tctatcgcct tcttgacgag ttcttctgac gtacgcgatg atctagagct cgctgatcag
     9541 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     9601 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     9661 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     9721 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     9781 cggaaagaac ctgcaactag tctcgagata tctagaccca gctttcttgt acaaagttgg
     9841 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     9901 taaaatcatt atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt
     9961 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
    10021 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
    10081 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
    10141 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
    10201 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
    10261 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
    10321 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
    10381 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
    10441 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
    10501 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
    10561 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
    10621 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
    10681 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
    10741 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
    10801 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
    10861 attggttgta acattattca gattgggccc cgttccactg agcgtcagac cccgtagaaa
    10921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11581 acatgtt
//
