LOCUS       sequence-212984-       11063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector carrying human EF1a promoter-driven 
            dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Chong ZS, Ohnishi S, Yusa K, Wright GJ
  TITLE     Pooled extracellular receptor-ligand interaction screening using 
            CRISPR activation.
  JOURNAL   Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
  PUBMED    30477585
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-11-26"; volume: "19(1):205. doi"; pages: " 
            10.1186/s13059-018-1581-3"
COMMENT     Sequence Label: pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
FEATURES             Location/Qualifiers
     source          1..11063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        908..2093
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1139..2085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2042..2062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2125..2172
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(2173..2196,2197..2199,2200..4755,4756..4758,
                     4759..6273)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6328..6348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6373..6522
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6553..6606
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6613..6622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6622..7008
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             7063..7083
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7099..7641
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7666..8037
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     misc_feature    join(8062..8635,8636..8638,8639..8647)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8229..8246)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8456..8475
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8648..9043
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9047..9271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(9292..9391)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9503..9522)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9514..10323
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9581..9600)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10413..11001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10902..10921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga
      481 ctggatccac gcgtgagggg cgcggaagcc ggggcggagg aggagccaac cccgggctgg
      541 gctgagaccc gcagaggaag acgctctagg gatttgtccc ggactagcga gatggcaagg
      601 ctgaggacgg gaggctgatt gagaggcgaa ggtacaccct aatctcaata caacctttgg
      661 agctaagcca gcaatggtag agggaagatt ctgcacgtcc cttccaggcg gcctccccgt
      721 caccaccccc cccaacccgc cccgaccgga gctgagagta attcatacaa aaggactcgc
      781 ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga
      841 tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca
      901 tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      961 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     1021 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1081 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     1141 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     1201 ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     1261 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     1321 ttgaggcctg gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     1381 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     1441 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tcgatctgca cactggtatt
     1501 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1561 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     1621 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     1681 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     1741 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1801 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1861 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1921 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1981 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     2041 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgggaagct
     2101 tggcgcgccc gtacggccac catgaaaagg ccggcggcca cgaaaaaggc cggccaggca
     2161 aaaaagaaaa aggacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
     2221 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     2281 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     2341 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     2401 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     2461 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     2521 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     2581 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     2641 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     2701 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     2761 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     2821 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     2881 ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     2941 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     3001 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     3061 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     3121 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     3181 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     3241 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     3301 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     3361 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     3421 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     3481 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     3541 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     3601 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     3661 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     3721 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     3781 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     3841 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     3901 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     3961 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     4021 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     4081 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     4141 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     4201 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     4261 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     4321 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     4381 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     4441 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     4501 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     4561 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     4621 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     4681 gtggaccaca tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     4741 accagaagcg acaaggcccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     4801 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     4861 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     4921 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     4981 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     5041 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     5101 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     5161 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     5221 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     5281 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     5341 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     5401 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     5461 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     5521 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     5581 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     5641 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     5701 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     5761 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     5821 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     5881 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     5941 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     6001 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     6061 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     6121 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     6181 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     6241 acacggatcg acctgtctca gctgggaggc gacagcgctg gaggaggtgg aagcggagga
     6301 ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctgga
     6361 tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc
     6421 gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg
     6481 ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaactg tacgggcggc
     6541 gggtccggag gagagggcag aggaagtctc ctaacatgcg gtgacgtgga ggagaatcct
     6601 ggcccatgta cggccaccat ggcttcaaac tttactcagt tcgtgctcgt ggacaatggt
     6661 gggacagggg atgtgacagt ggctccttct aatttcgcta atggggtggc agagtggatc
     6721 agctccaact cacggagcca ggcctacaag gtgacatgca gcgtcaggca gtctagtgcc
     6781 cagaagagaa agtataccat caaggtggag gtccccaaag tggctaccca gacagtgggc
     6841 ggagtcgaac tgcctgtcgc cgcttggagg tcctacctga acatggagct cactatccca
     6901 attttcgcta ccaattctga ctgtgaactc atcgtgaagg caatgcaggg gctcctcaaa
     6961 gacggtaatc ctatcccttc cgccatcgcc gctaactcag gtatctacag cgctggagga
     7021 ggtggaagcg gaggaggagg aagcggagga ggaggtagcg gacctaagaa aaagaggaag
     7081 gtggcggccg ctggatcccc ttcagggcag atcagcaacc aggccctggc tctggcccct
     7141 agctccgctc cagtgctggc ccagactatg gtgccctcta gtgctatggt gcctctggcc
     7201 cagccacctg ctccagcccc tgtgctgacc ccaggaccac cccagtcact gagcgctcca
     7261 gtgcccaagt ctacacaggc cggcgagggg actctgagtg aagctctgct gcacctgcag
     7321 ttcgacgctg atgaggacct gggagctctg ctggggaaca gcaccgatcc cggagtgttc
     7381 acagatctgg cctccgtgga caactctgag tttcagcagc tgctgaatca gggcgtgtcc
     7441 atgtctcata gtacagccga accaatgctg atggagtacc ccgaagccat tacccggctg
     7501 gtgaccggca gccagcggcc ccccgacccc gctccaactc ccctgggaac cagcggcctg
     7561 cctaatgggc tgtccggaga tgaagacttc tcaagcatcg ctgatatgga ctttagtgcc
     7621 ctgctgtcac agatttcctc tagtgggcag ggaggaggtg gaagcggctt cagcgtggac
     7681 accagtgccc tgctggacct gttcagcccc tcggtgaccg tgcccgacat gagcctgcct
     7741 gaccttgaca gcagcctggc cagtatccaa gagctcctgt ctccccagga gccccccagg
     7801 cctcccgagg cagagaacag cagcccggat tcagggaagc agctggtgca ctacacagcg
     7861 cagccgctgt tcctgctgga ccccggctcc gtggacaccg ggagcaacga cctgccggtg
     7921 ctgtttgagc tgggagaggg ctcctacttc tccgaagggg acggcttcgc cgaggacccc
     7981 accatctccc tgctgacagg ctcggagcct cccaaagcca aggaccccac tgtctcctgt
     8041 acatgagaat tccgcccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     8101 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     8161 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     8221 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     8281 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     8341 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     8401 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     8461 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     8521 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt
     8581 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     8641 cacaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     8701 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     8761 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     8821 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     8881 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     8941 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     9001 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggccgactgt gccttctagt
     9061 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     9121 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     9181 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agtcaatagc
     9241 aggcatgctg gggatgcggt gggctctatg gactagtctc gagatatcta gacccagctt
     9301 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     9361 tcactatcag tcaaaataaa atcattattt gccatccagc tgcagctctg gcccgtgtct
     9421 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     9481 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     9541 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     9601 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     9661 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     9721 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     9781 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     9841 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     9901 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     9961 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
    10021 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
    10081 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
    10141 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
    10201 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
    10261 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
    10321 taatcagaat tggttaattg gttgtaacat tattcagatt gggccccgtt ccactgagcg
    10381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11041 tgctggcctt ttgctcacat gtt
//
