LOCUS       sequence-213000-        5763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP12-LASP1-C-10
FEATURES             Location/Qualifiers
     source          1..5763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             667..1626
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     regulatory      1877..1886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2605..2726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2642..2661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2696..2715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2733..3188)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2870..2891)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3082..3101
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3215..3319
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3321..3678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3321..3512
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3344..3364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3529..3664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3713..4507
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3713..4507
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3767..3786)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4377..4396
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4695..4714)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4739..4786
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5115..5703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5604..5623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatgt cggtaccgct gactacctca gcattcggcc acgcgtttct ggctaactgt
      721 gaacgcgagc agatccacct ggcgggctcc attcagccgc acggtatcct gctggctgtg
      781 aaagagccgg acaacgtggt gatccaggct tctattaacg ctgcggagtt cctgaacacc
      841 aactctgttg ttggccgtcc gctgcgtgac ctgggcggcg atctgccttt gcagatcctg
      901 ccgcacctga acggcccgct gcacctggct ccgatgaccc tgcgttgtac cgtgggttct
      961 ccgccgcgtc gtgtggactg taccattcat cgtccgtcta acggcggcct gatcgtagaa
     1021 ctggaaccag caaccaagac cactaacatt gcgccggctc tggacggtgc gtttcatcgt
     1081 atcacttctt catcctccct gatgggcctg tgtgacgaaa ccgcgactat tatccgtgag
     1141 attactggct acgaccgtgt gatggtagta cgtttcgatg aagagggtaa tggcgaaatt
     1201 ctgtccgaac gtcgtcgtgc ggacctggaa gcgttcctgg gtaaccgcta cccggcgtct
     1261 actattccgc agatcgctcg tcgcctgtac gaacataacc gtgttcgcct gctggtagat
     1321 gtgaactata ctccggttcc gctacagccg cgcatcagcc cgctgaacgg tcgtgatctg
     1381 gatatgtccc tgtcttgcct gcgctctatg tccccgatcc accagaaata catgcaggac
     1441 atgggcgttg gcgcgaccct ggtttgctct ctgatggtgt ctggtcgtct gtggggtctg
     1501 atcgcttgcc accactacga accgcgcttc gttccgttcc acattcgcgc tgctggcgaa
     1561 gcgctggcgg aaacttgtgc gatccgcatc gcgacgctgg agagctttgc acagtctcag
     1621 tccaaaggtg gcggaagcgg cggcggaagc ggtggcggaa gttccggact cagatctagc
     1681 ggtggcagtg gaaaccccaa ctgcgcccgg tgcggcaaga tcgtgtatcc cacggagaag
     1741 gtgaactgtc tggataagtt ctggcataaa gcatgcttcc attgcgagac ctgcaagatg
     1801 acactgaaca tgaagaacta caagggctac gagaagaagc cctactgcaa cgcacactac
     1861 cccaagcagt ccttcaccat ggtggcggac accccggaaa accttcgcct caagcaacag
     1921 agtgagctcc agagtcaggt gcgctacaag gaggagtttg agaagaacaa gggcaaaggt
     1981 ttcagcgtag tggcagacac gcccgagctc cagagaatca agaagaccca ggaccagatc
     2041 agtaacataa aataccatga ggagtttgag aagagccgca tgggccctag cgggggcgag
     2101 ggcatggagc cagagcgtcg ggattcacag gacggcagca gctaccggcg gcccctggag
     2161 cagcagcagc ctcaccacat cccgaccagt gccccggttt accagcagcc ccagcagcag
     2221 ccggtggccc agtcctatgg tggctacaag gagcctgcag ccccagtctc catacagcgc
     2281 agcgccccag gtggtggcgg gaagcggtac cgcgtggtgt atgactacag cgccgccgac
     2341 gaggacgagg tctccttcca ggacggggac accatcgtca acgtgcagca gatcgacgac
     2401 ggctggatgt acgggacggt ggagcgcacc ggcgacacgg ggatgctgcc ggccaactac
     2461 gtggaggcca tctgaggatc caccggatct agataactga tcataatcag ccataccaca
     2521 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2581 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2701 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     2761 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     2821 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     2881 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcaaaaaa ccgtctatca
     2941 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3001 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3061 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3121 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3181 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3301 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3361 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3421 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3481 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3541 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3601 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3661 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3721 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3781 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3841 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     3901 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     3961 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4021 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4081 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4141 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4201 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4261 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4321 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4381 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4441 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4501 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4561 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4621 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4681 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4741 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     4801 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     4861 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     4921 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     4981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5761 atg
//
