LOCUS       sequence-21302-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-dependent expression of DsRed, i.e. DsRed is only 
            expressed in the presence of Cre due to a transcriptional stop in 
            between the loxP sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCALNL-DsRed
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..736)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(121..144)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     536..556
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      733..742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    814..847
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(885..904)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     939..958
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag tgcggccgct
       61 acaggaacag gtggtggcgg ccctcggtgc gctcgtactg ctccacgatg gtgtagtcct
      121 cgttgtggga ggtgatgtcc agcttggcgt ccacgtagta gtagccgggc agctgcacgg
      181 gcttcttggc catgtagatg gacttgaact ccaccaggta gtggccgccg tccttcagct
      241 tcagggcctt gtgggtctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg
      301 aggcctccca gcccatggtc ttcttctgca tcacggggcc gtcggagggg aagttcacgc
      361 cgatgaactt caccttgtag atgaagcagc cgtcctgcag ggaggagtcc tgggtcacgg
      421 tcgccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
      481 acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac gtacaccttg gagccgtact
      541 ggaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
      601 gcttcacggt gttgtggccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
      661 cgtggccgtt cacggtgccc tccatgcgca ccttgaagcg catgaactcg gtgatgacgt
      721 tctcggagga ggccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
      781 gaattcaatt taaatcgtac cgagcgactc gagataactt cgtataatgt atgctatacg
      841 aagttatacg cgttcggatt gatccagaca tgataagata cattgatgag tttggacaaa
      901 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      961 tatttgtaac catta
//
