LOCUS       sequence-213041-       14231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABA signaling reporter, GFP under the RAB18 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Kim TH, Hauser F, Ha T, Xue S, Bohmer M, Nishimura N, Munemasa S, 
            Hubbard K, Peine N, Lee BH, Lee S, Robert N, Parker JE, Schroeder JI
  TITLE     Chemical genetics reveals negative regulation of abscisic acid 
            signaling by a plant immune response pathway.
  JOURNAL   Curr Biol. 2011 Jun 7;21(11):990-7. doi: 10.1016/j.cub.2011.04.045. 
            Epub 2011 May 27.
  PUBMED    21620700
REFERENCE   2  (bases 1 to 14231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2011.04.045"; journalName: "Curr Biol"; date: 
            "2011-06-7- 7"; volume: "21"; issue: "11"; pages: "990-7"
COMMENT     Sequence Label: pRAB18-GFP
FEATURES             Location/Qualifiers
     source          1..14231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6441..6443,6444..6446,6447..7160)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(6486..6507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6747..6766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7094..7115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7179..7431
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7461..7478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7461..7477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7470..7492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7617..7636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7827..7848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8095..8119
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9715..10863)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11162..11956)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12148..12915
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(13608..14231,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agctttccag acacatcttg ggattagatc
     4981 atagtccata ctaatgaaga caaaagtgtt aaccaaatat agataatgag agaatgtggg
     5041 gggtgaagtt gaaccctgag tgctgcaatc ctatctgcgt gcaacttttc tagctctgga
     5101 tcctacagaa caagagcaag tcagtctccc atagagacag aagagaaaca taacatttca
     5161 ttgtacagat tataaaagaa caattcaaag agagccccca ttgaacttac atccatcaat
     5221 tcgtcaagat caacttcctc gttgacaggt cttgatcctt gtgccttttc atttgcaaga
     5281 acttcctgca caacaaaaat acggaaatgt ttttgatcca agaacatcaa actctaattg
     5341 caattgtaac caacaaaaaa acacttaaaa ttcgctatac actatcaaat ttcctgacac
     5401 gtagacctca tgtcagcaca aatagaatct agcctatctc tgttaattgg gttaccaaac
     5461 aacatgagat tgattatgtg gaaaaccaag cacattattt accctttgaa aatacgcgaa
     5521 atcaagatcc aagaagaatt tatggagaac agcttcaaag tacgaaataa acaatgaaga
     5581 ttaaattacc tttttataat ctctagcagc tgccgccaat acattcccga atgccagatt
     5641 cgagagggtc gacttcaccg tatccggatc catctcttta ccaaccaact aatccaactc
     5701 agaaaatttt aaaatctcaa tcaaaaatcc ctctaagata gccagagaag agattgtaaa
     5761 caaggatttg aaatctggtg cagagaggag aaactccccg acaatgaaca ccaacgatct
     5821 aaacgcggcg tttggtaaaa gttgagtaaa ttttgttagg gcttagtttt agtccatggg
     5881 ctaattagta agtgatttac ggcccacaca tgagcccaaa tgtttcagac ccagccaagt
     5941 ttcttcaaat tcacccaatc aacgacgatg tacgtgtgta tgaaaatcat taacacgacg
     6001 catcgctttc gaggaggagc attacgtgtc ctgttagcta cgataatgtt agtaccgcca
     6061 caaagaaaag gatagatatt ttgctttcca gcaccctgtc atgggattga tatgaacacg
     6121 tacttggtat cgacatgaaa gctcaaaaat aaattcaatc cgattccttt agtgatatca
     6181 gaagttcatt ttaaatacga acacgtatgg cgaaacacca cgccgacatt ttctgctgct
     6241 gccacgcgtc actttccaaa tattgattca ttaaactaat agttgatcca tatccgaaac
     6301 cggactataa aactatcttc aatgcgttaa cgaatcttca tcgatcaaac tcatcaaagt
     6361 ctaatatcac aaagaaagag tttttttaac tagcttagct caaagtgttt gcttaagaca
     6421 agaagaactc tagaggatcc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     6481 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     6541 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6601 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc
     6661 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6721 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6781 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6841 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6901 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     6961 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     7021 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     7081 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca
     7141 tggacgagct gtacaagtaa agcggccgcc cggctgcaga tcgttcaaac atttggcaat
     7201 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     7261 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     7321 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     7381 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat ccgatgataa
     7441 gctgtcaaac atgagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7501 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7561 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc
     7621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7741 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7861 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc
     7921 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     7981 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     8041 ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     8101 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     8161 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     8221 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     8281 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     8341 gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta
     8401 ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg
     8461 aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt
     8521 tgacgatatc aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt
     8581 tccgactgtc ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc
     8641 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     8701 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     8761 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     8821 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     8881 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
     8941 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
     9001 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
     9061 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
     9121 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
     9181 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
     9241 gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat
     9301 gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct
     9361 cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca
     9421 tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg
     9481 ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag
     9541 ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt
     9601 gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa
     9661 tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
     9721 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
     9781 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
     9841 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
     9901 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
     9961 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
    10021 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
    10081 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
    10141 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
    10201 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
    10261 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
    10321 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
    10381 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
    10441 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
    10501 cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
    10561 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
    10621 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
    10681 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga
    10741 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
    10801 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt
    10861 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
    10921 atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
    10981 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
    11041 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
    11101 gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt
    11161 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
    11221 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
    11281 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
    11341 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
    11401 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
    11461 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
    11521 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
    11581 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
    11641 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
    11701 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
    11761 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
    11821 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
    11881 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
    11941 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
    12001 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
    12061 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    12121 ggagccgatt ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg
    12181 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
    12241 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
    12301 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
    12361 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat
    12421 gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc
    12481 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc
    12541 tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag
    12601 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
    12661 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
    12721 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
    12781 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
    12841 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
    12901 gttggagtca ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt
    12961 tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc
    13021 gcgctgccta tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag
    13081 atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc
    13141 atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt
    13201 ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg
    13261 tttatcgcac ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag
    13321 gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc
    13381 aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc
    13441 agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt
    13501 actgccccga tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg
    13561 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag
    13621 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
    13681 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
    13741 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
    13801 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
    13861 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
    13921 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
    13981 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
    14041 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
    14101 ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt
    14161 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    14221 aaccagcccg g
//
