LOCUS       sequence-213050-        6008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6008)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2039..2048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2045..2728
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2850..2971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2887..2906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2941..2960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2978..3433)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3115..3136)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3327..3346
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3460..3564
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3566..3923
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3566..3757
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3589..3609)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3774..3909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3958..4752
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3958..4752
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4012..4031)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4622..4641
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4940..4959)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4984..5031
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5360..5948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5849..5868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattcgg cagcactttt ctttcattgg ggggaaggcg
      721 tgaggaaagt accaaacagc agcagacttt taaactttaa acagacaggt ctgagagcct
      781 gaactctcat ttttcctttg acttcagcct ccaaggagtt ccaccaactt tggcgcgccg
      841 gcttcacttt cattaagtga aagagaggtg cccagacatg ggtgactgga gtgccttggg
      901 gaagcttctg gacaaggtcc aagcctactc caccgctgga gggaaggtgt ggctgtcagt
      961 gctcttcata ttcagaatcc tgctcctggg gacagctgtt gagtcagctt ggggtgatga
     1021 acagtctgcc tttcgctgta acactcaaca acctggctgc gaaaacgtct gctatgacaa
     1081 gtccttcccc atctctcacg tgcgcttctg ggtccttcag atcatattcg tgtctgtgcc
     1141 cacactcctg tacttggccc atgtgttcta tgtgatgagg aaggaagaga agctaaacaa
     1201 gaaagaagag gagctcaaag tggcccagac tgacggggtc aacgtggaga tgcacctgaa
     1261 gcagattgaa atcaagaagt tcaagtacgg gattgaagag cacggcaagg tgaaaatgag
     1321 gggcggcttg ctgagaacct acatcatcag catcctcttc aagtctgtct tcgaggtggc
     1381 cttcctgctc atccagtggt acatctatgg gttcagcttg agcgcggtct acacctgcaa
     1441 gagagatccc tgcccccacc aggtagactg cttcctctca cgtcccacgg agaaaaccat
     1501 cttcatcatc ttcatgctgg tggtgtcctt ggtgtctctc gctttgaaca tcattgagct
     1561 cttctacgtc ttcttcaagg gcgttaagga tcgcgtgaag ggaagaagcg atccttacca
     1621 cgccaccact ggcccactga gcccatcaaa agactgcgga tctccaaaat acgcctactt
     1681 caatggctgc tcctcaccaa cggctccact ctcgcctatg tctcctcctg ggtacaagct
     1741 ggttactggt gacagaaaca attcctcgtg ccgcaattac aacaagcaag ctagcgagca
     1801 aaactgggcg aactacagcg cagagcaaaa tcgcatgggg caggccggaa gcaccatctc
     1861 caactcgcac gcccagccgt tcgatttccc cgacgacaac cagaatgcca aaaaagttgc
     1921 tgctggacat gaactccagc cattagccat cgtggaccaa cgaccttcca gcagagccag
     1981 cagccgcgcc agcagcaggc ctcggcctga tgacctggag attgcggatc caccggtcgc
     2041 caccatggtg agtgcgatta agccagacat gaggatcaaa ctccgtatgg aaggcaacgt
     2101 aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttatg agggaaaaca
     2161 gaccatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac
     2221 cactgcattc cattacggca acagggtatt cgttaaatat ccagacaaca tacaagacta
     2281 ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg
     2341 gggcatttgc tatgccagaa acgacataac aatggaaggg gacactttct ataataaagt
     2401 tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa
     2461 atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatattca
     2521 tatggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa
     2581 agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccacg ccattgagat
     2641 tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc
     2701 tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata
     2761 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     2821 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2881 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2941 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     3001 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3061 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3121 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3181 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3241 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     3301 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     3361 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     3421 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3481 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3541 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     3601 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3661 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3721 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     3781 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3841 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3901 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     3961 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4021 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4081 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4141 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4201 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4261 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4321 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4381 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4441 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     4501 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4561 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4621 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     4681 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     4741 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     4801 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     4861 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     4921 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     4981 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     5041 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5101 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5161 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5941 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6001 ctcacatg
//
