LOCUS       sequence-213052-        5880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5880)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..5880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        230..433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     384..404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1915..1924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1921..2604
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2726..2847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2763..2782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2817..2836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2854..3309)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2991..3012)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3203..3222
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3336..3440
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3442..3799
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3442..3633
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3465..3485)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3650..3785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3834..4628
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3834..4628
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3888..3907)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4498..4517
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4816..4835)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4860..4907
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5236..5824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5725..5744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      241 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      301 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      361 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      421 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
      481 gagctcaagc ttcgaattcc catgtccacc aggtccgtgt cctcgtcctc ctaccgcagg
      541 atgttcggcg gcccgggcac cgcgagccgg ccgagctcca gccggagcta cgtgactacg
      601 tccacccgca cctacagcct gggcagcgcg ctgcgcccca gcaccagccg cagcctctac
      661 gcctcgtccc cgggcggcgt gtatgccacg cgctcctctg ccgtgcgcct gcggagcagc
      721 gtgcccgggg tgcggctcct gcaggactcg gtggacttct cgctggccga cgccatcaac
      781 accgagttca agaacacccg caccaacgag aaggtggagc tgcaggagct gaatgaccgc
      841 ttcgccaact acatcgacaa ggtgcgcttc ctggagcagc agaataagat cctgctggcc
      901 gagctcgagc agctcaaggg ccaaggcaag tcgcgcctgg gggacctcta cgaggaggag
      961 atgcgggagc tgcgccggca ggtggaccag ctaaccaacg acaaagcccg cgtcgaggtg
     1021 gagcgcgaca acctggccga ggacatcatg cgcctccggg agaaattgca ggaggagatg
     1081 cttcagagag aggaagccga aaacaccctg caatctttca gacaggatgt tgacaatgcg
     1141 tctctggcac gtcttgacct tgaacgcaaa gtggaatctt tgcaagaaga gattgccttt
     1201 ttgaagaaac tccacgaaga ggaaatccag gagctgcagg ctcagattca ggaacagcat
     1261 gtccaaatcg atgtggatgt ttccaagcct gacctcacgg ctgccctgcg tgacgtacgt
     1321 cagcaatatg aaagtgtggc tgccaagaac ctgcaggagg cagaagaatg gtacaaatcc
     1381 aagtttgctg acctctctga ggctgccaac cggaacaatg acgccctgcg ccaggcaaag
     1441 caggagtcca ctgagtaccg gagacaggtg cagtccctca cctgtgaagt ggatgccctt
     1501 aaaggaacca atgagtccct ggaacgccag atgcgtgaaa tggaagagaa ctttgccgtt
     1561 gaagctgcta actaccaaga cactattggc cgcctgcagg atgagattca gaatatgaag
     1621 gaggaaatgg ctcgtcacct tcgtgaatac caagacctgc tcaatgttaa gatggccctt
     1681 gacattgaga ttgccaccta caggaagctg ctggaaggcg aggagagcag gatttctctg
     1741 cctcttccaa acttttcctc cctgaacctg agggaaacta atctggattc actccctctg
     1801 gttgataccc actcaaaaag gacacttctg attaagacgg ttgaaactag agatggacag
     1861 gttatcaacg aaacttctca gcatcacgat gaccttgaag gggatccacc ggtcgccacc
     1921 atggtgagtg cgattaagcc agacatgagg atcaaactcc gtatggaagg caacgtaaac
     1981 gggcaccact ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc
     2041 atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact
     2101 gcattccatt acggcaacag ggtattcgtg aaatatccag acaacataca agactatttt
     2161 aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc
     2221 atttgcaatg ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga
     2281 ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg
     2341 gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tattgagatg
     2401 gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct
     2461 aaggagaagg gtgtcaagtt accaggcgcc cactttgtgg accacgccat tgagatttta
     2521 agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga
     2581 ttgcctgaca atgccagacg ataagcggcc gcgactctag atcataatca gccataccac
     2641 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2701 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2821 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     2881 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     2941 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3001 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3061 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3121 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3181 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3241 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3301 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3361 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3421 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3481 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3541 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3601 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3661 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3721 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3781 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     3841 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3901 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3961 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4021 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4081 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4141 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4201 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4261 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4321 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4381 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4441 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4501 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4561 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4621 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4681 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4741 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4801 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     4861 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     4921 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     4981 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5041 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5101 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
//
