LOCUS       sequence-213055-       10003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac compatible plasmid expressing dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC,
            Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET, 
            Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church 
            GM
  TITLE     An enhanced CRISPR repressor for targeted mammalian gene regulation.
  JOURNAL   Nat Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 
            10.1038/s41592-018-0048-5.
  PUBMED    30013045
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Jul 16. pii: 10.1038/s41592-018-0048-5. doi: 
            10.1038/s41592-018-0048-5."
COMMENT     Sequence Label: pB-CAGGS-dCas9
FEATURES             Location/Qualifiers
     source          1..10003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(103..129,130..132,133..2616,2617..2622,2623..2688,
                     2689..2691,2692..4206)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4219..4239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4264..4283)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4308..4356
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4588..4799
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4809..4818
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4815..5213
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5217..5338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5254..5273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5308..5327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6101..6135
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6626..6642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6626..6642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6639..6661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6650..6666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6674..6680,6681..6698,6699..6704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6719..6740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6857..6874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7028..7616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7108..7127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7787..8578,8579..8647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8410..8429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8648..8752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8778..9233)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8915..8936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9127..9146
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9360..9382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9374..9391
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9375..9391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9521..9583
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga atctagatac
       61 cctcgtaaag gtctagagct agcggatcga acccttgcca ccatggacaa gaagtactcc
      121 attgggctcg ctatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag
      181 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
      241 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
      301 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
      361 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
      421 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
      481 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
      541 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
      601 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
      661 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
      721 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
      781 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
      841 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
      901 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
      961 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
     1021 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat
     1081 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
     1141 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
     1201 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
     1261 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
     1321 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
     1381 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
     1441 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
     1501 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc
     1561 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
     1621 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
     1681 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa
     1741 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
     1801 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
     1861 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
     1921 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
     1981 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
     2041 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
     2101 atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct taagtccgat
     2161 ggatttgcca accggaactt catgcagttg atccatgatg actctctcac ctttaaggag
     2221 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat
     2281 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
     2341 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
     2401 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
     2461 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
     2521 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
     2581 gaactggaca tcaatcggct ctccgactac gacgtggctg ctatcgtgcc ccagtctttt
     2641 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaagc tagagggaag
     2701 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
     2761 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
     2821 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
     2881 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
     2941 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc
     3001 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
     3061 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
     3121 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
     3181 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
     3241 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
     3301 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
     3361 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
     3421 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
     3481 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
     3541 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
     3601 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
     3661 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
     3721 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg
     3781 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
     3841 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
     3901 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
     3961 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
     4021 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
     4081 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
     4141 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
     4201 ggagacagca gggctgaccc caagaagaag aggaaggtgt gagtttaaac gggggaggct
     4261 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     4321 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     4381 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     4441 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     4501 gggcggcagg ccctgccata gcagatctgc gaaatcggat ccgcggccgc actgattcta
     4561 gctgcagagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4621 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     4681 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4741 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4801 atcctcgagc caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     4861 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     4921 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     4981 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     5041 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     5101 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     5161 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagttaact
     5221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5341 atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc tggtctccct
     5401 tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa actgggttct
     5461 tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag gaacgaagtc
     5521 cctaaagaaa cagtggcagc caggtttagc cccggaattg actggattcc ttttttaggg
     5581 cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     5641 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     5701 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     5761 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     5821 gtccctgata tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
     5881 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
     5941 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     6001 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     6061 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     6121 actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg
     6181 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     6241 tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac
     6301 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     6361 aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     6421 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     6481 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     6541 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     6601 ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8761 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8821 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8881 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8941 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9001 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9061 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9121 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9181 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     9241 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     9301 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9361 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttttga
     9421 acccgtggag gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat
     9481 gaagatgctc gacacgctgc agaacacgca gctagattaa ccctagaaag ataatcatat
     9541 tgtgacgtac gttaaagata atcatgtgta aaattgacgc atgtgtttta tcggtctgta
     9601 tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt acacttacat
     9661 actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaacaaa
     9721 aactcaaaat ttcttctata aagtaacaaa acttttatga gggacagccc ccccccaaag
     9781 cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc
     9841 tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg
     9901 ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc
     9961 ctgcagacac ctggggggat acggggaaaa ggcctccacg gcc
//
