LOCUS       sequence-213057-        8136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g02385 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g02385-bio-His
FEATURES             Location/Qualifiers
     source          1..8136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3300..3317
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3369..3424
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3423..3442)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5660..5678)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5746..5850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5851..5919,5920..6711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6069..6088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6882..7470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7371..7390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7624..7641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7758..7779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7794..7799,7800..7817,7818..7824)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7832..7848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7837..7859
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7856..7872
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7856..7872
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 atctgcctat ccacgccctg accaacgaac tcgtgggcaa ctggaaggtg tacctgacaa
     1261 acacccacag cgagaagttt ctgaactgcg gcggcagcag ccccaacaac aacgccgcca
     1321 acctgcacca cagcctgaac gactacaagc ggttcctgct ggataagtac ggcaagctga
     1381 ccgagtacga cgtgaacttc gccgtggaaa gaagcgtgga ccactccctg gtgttccccc
     1441 ggaacaagtg gaagctgctg aacatcctgg accagaagca caacatcatc ggccactgga
     1501 ccatggtgta cgatgtgggc ctgaatatcc ggatgtgcaa gatcgaggcc ttcggctacc
     1561 tgcggtacac caagggcaac aaggacgagt gcgtggacag cggcaacggc aagaaccggg
     1621 ccaaggatga gttcgactgc tacaagaccg accccaccaa gatccacatc ggctgggcca
     1681 gacgggacaa cggcgccttc gagtgcttct acggcgagaa gatcggcaat gtggaaggca
     1741 tgggcggctc caccgtgctg ttcaagaaca tggccatgga cgagcggttc aaggtgtccg
     1801 gcgacaacgt ggccatcaac agcgaggcca gccacaagta ccacctgttc ggcaatcagg
     1861 tgtacgcctg cggcagaccc taccccatgt ttaagaccga gagcctgccc aagaattgga
     1921 agtggtccga cctggaactg gacagcagcg agacaggcaa ccagggcgcc tgtggcagct
     1981 gttacgccct ggccagcatc tgggccatcg agaagagatt cgagatcggc tacaaaaaga
     2041 tgatgaacaa ggcctaccgg atcgagtttg atgtgaacag cctcgtgcgg aagtccccct
     2101 acaaccagga atgcaagggc ggatacccat tcctcgtggg aaaggacatc aaggacttcg
     2161 gcatcaaggc cctgaacccc gtgttcagcg accggttcta cgtgaaggat tacaactacg
     2221 tgggcggatg ctacgagtgc accaacgagg ctctgatgat gcacgagatc tacaacttcg
     2281 gccccatcgt ggccgccatc aatgccggcc ctgacttcat ctcccacaag aagggcatct
     2341 tcgagggcga aaccgagatc gagcggaaga ggttctgcga cagccccgat accggcctgg
     2401 gaggctggga gttcaccaac cacgccgtgg ctatcatcgg atggggcgag tccgagaacg
     2461 acggcaagta ctgggtcatc aagaacgact ggggcggaga ttggggcgtg aacggctacg
     2521 ccaaagtgcg gagaggcatc aacctgaacg gcatcgagag ccaggccatc tacttcgacc
     2581 ccgacttcga gagcggcgga ggcaacgaga tcctgactaa gaacagccgc agcaccgtgc
     2641 accggaccgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     2701 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     2761 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     2821 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     2881 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     2941 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     3001 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     3061 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     3121 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     3181 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     3241 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     3301 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     3361 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3421 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     3481 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     3541 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     3601 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     3661 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     3721 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     3781 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     3841 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     3901 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     3961 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     4021 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     4081 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     4141 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     4201 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     4261 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     4321 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     4381 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     4441 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     4501 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     4561 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     4621 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     4681 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     4741 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     4801 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     4861 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     4921 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     4981 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     5041 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     5101 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5161 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     5221 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     5281 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     5341 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     5401 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5461 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     5521 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5581 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     5641 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5701 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5821 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5881 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5941 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6001 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6061 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6121 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6181 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6241 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6301 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6361 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     6421 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6481 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6541 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6601 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6661 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6721 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6781 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6841 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6901 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6961 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7021 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7081 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7141 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7201 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7261 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7321 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7381 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7441 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7501 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7561 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7621 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7681 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7741 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     7801 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     7861 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     7921 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     7981 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     8041 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     8101 tgtacattta tattggctca tgtccaatat gaccgc
//
