LOCUS       sequence-213061-        7446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g04855 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g04855-bio-His
FEATURES             Location/Qualifiers
     source          1..7446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2610..2627
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2679..2734
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2733..2752)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4970..4988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5056..5160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5161..5229,5230..6021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5379..5398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6192..6780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6681..6700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6934..6951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7068..7089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7104..7109,7110..7127,7128..7134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7142..7158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7147..7169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7166..7182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7166..7182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 ctcccagcaa tggcctgtac gagagcaacc tgttctacac cgagggctac ggcaagtacc
     1261 tgaccagccc caccaagatc aagaccatcg agttcggcgg ctacaagttc gagtttgacg
     1321 acgacaccct gcccgtgacc agcatcacaa agatcgacgt gatcacctac gacgataagc
     1381 ccatcctgtt tgagttcatc agcgacaagg accggcccta ccggcggttc tactactaca
     1441 ccctggacag caagaccaac aagctgtaca actacgtgac cgccgagaca ggctacaacg
     1501 tggaagatag cagcggcctg aagtactaca cagagctgag caagagcggc atcaacgacg
     1561 tgctgcagga cctggacaag aacatcgacg agtccaacat cgagcacctg aaaaccagct
     1621 atgtgaccaa gggcctgaat atcgccatcg aggtgtacag caaccgggtg gtggaacaga
     1681 tcaagtctat caaggtggtc acccccgtgg aactgttcga ctacaagacc gaggtgccca
     1741 tcgagagcgt ggaccacgag agccgggaca acagcctggc cgaggtggaa gaggacggca
     1801 aggccgtgca ggtcggaacc cagcccgtgt acgaagtgaa cgacggcgcc cacaaccctg
     1861 ccgcccaggt gctgagccag aacaacatca tcgagacact ggacgacaag agcaaagtga
     1921 cccacctgag aaacgccggc agcgagaaga tcagagtggg cgcgccgtcg acctccatca
     1981 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     2041 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     2101 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     2161 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     2221 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     2281 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     2341 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     2401 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     2461 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     2521 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     2581 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     2641 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     2701 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2761 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     2821 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     2881 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     2941 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3001 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3061 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3121 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     3181 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     3241 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     3301 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     3361 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     3421 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     3481 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     3541 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     3601 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     3661 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     3721 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     3781 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     3841 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     3901 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     3961 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4021 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4081 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4141 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     4201 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     4261 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     4321 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     4381 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4441 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4501 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4561 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     4621 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     4681 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     4741 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4801 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     4861 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     4921 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     4981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5161 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5221 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5281 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5341 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5401 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5461 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5521 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5581 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5641 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5701 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5761 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5881 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5941 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6961 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7021 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7081 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7141 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     7201 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     7261 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     7321 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     7381 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     7441 gaccgc
//
