LOCUS       sequence-213063-        7668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g00690 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g00690-bio-His
FEATURES             Location/Qualifiers
     source          1..7668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2832..2849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2901..2956
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2955..2974)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5192..5210)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5278..5382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5383..5451,5452..6243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5601..5620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6414..7002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6903..6922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7156..7173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7290..7311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7326..7331,7332..7349,7350..7356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7364..7380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7369..7391
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7388..7404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7388..7404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tcctgttcga cacctacctg caggtcgtgt ccgagaacga ctgcaacccc aagtgcctgc
     1261 agggccacac ctgtatcctg aacagaaaga ccaacaagaa gtcctgcacc tgtcccccca
     1321 accactacta cgacgagaac ttcggctgcc agatggtcat cacatgcccc ctgtgccggc
     1381 acgccaaccc ttggggcacc acccacaccg tgcccaatac cgccctgccc ggccagcaca
     1441 agagcggcta ccagtacagc atctgcaagg acggccacac aaaggacgag atgcggacct
     1501 tctgccggcg gtacaacgcc tgcgagagag gcgccaagat ctgccccgag cacagcacat
     1561 gcatcatcga caacaagggc cacgccgtgt gcaacctgga caacggctac cggtggcacg
     1621 acaacaccaa gaaaggcgcc gtgcggatcg agtactgtgg cggccataac aagaacaagt
     1681 gcatccctcc cgccacctgt caggaagtga acaacgccaa cagcaacacc ctgaccgtgt
     1741 gtagctgccc cagcgagatg tacctgaccc ggaacaagcg gcagtgcagc aagcagcagc
     1801 agttcagcga taacaaggtg tactccatca gcgtgaagaa ccgggccgag aagttccccg
     1861 agaatttcaa ggtgttcctg gacggctgct tcgacgtgca gctggatggc aaagaaggcg
     1921 tggtcatcta caagagcagc gacaccgtgt ccagcatcaa gtccatcgtg aacatcccca
     1981 ccgacctgaa gtctatccac ttcgagatga agttcaagac cctgaacatc ttcgtcgaga
     2041 acggcaacga catgcaccct atcttctcca tcaagttcga ccacagcgat tgcaagttca
     2101 tcgagagcat cgagggcctg gaaatcggcg acgacatcct gcggaccgag agcaacatgc
     2161 gggacctgca ccccaccctg ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     2221 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     2281 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     2341 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     2401 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     2461 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     2521 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     2581 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     2641 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     2701 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     2761 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     2821 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     2881 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2941 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     3001 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     3061 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     3121 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     3181 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     3241 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     3301 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     3361 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     3421 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     3481 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     3541 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     3601 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     3661 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     3721 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     3781 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     3841 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     3901 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     3961 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     4021 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     4081 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     4141 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     4201 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     4261 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     4321 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     4381 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     4441 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     4501 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     4561 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4621 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4681 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     4741 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     4801 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     4861 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     4921 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     4981 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     5041 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     5101 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     5161 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5221 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     5641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     5941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7141 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7261 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7321 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7381 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     7441 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     7501 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     7561 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     7621 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgc
//
