LOCUS       sequence-213066-       11424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g02515 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g02515-bio-His
FEATURES             Location/Qualifiers
     source          1..11424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6588..6605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6657..6712
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6711..6730)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8948..8966)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9034..9138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9139..9207,9208..9999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9357..9376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10170..10758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10659..10678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10912..10929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11046..11067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11082..11087,11088..11105,11106..11112)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11120..11136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11125..11147
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11144..11160
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11144..11160
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgcccgtgga caccatcaag aaccaggcca accagcctat ccagcccaac ctggccgatc
     1261 agcccaatca gctgagcagc gccgaccagt acgacatcct gcggaccaac agcgccacct
     1321 ccagaagcaa cacccccagc gacgacacca gcccctccga caacgccgtg gacgactcca
     1381 agaacgagct gatggtgcag gcccagaaca ccctgttcac cgaccccaag cccagcagcc
     1441 tgtttggcgg ccagagcgcc tttgccagca gcgtgtccag agagctggaa aacggcatcc
     1501 ctaagtacgc cgccaagaat gccctgaccc ggggcagcaa ctactggaag tccgagcaga
     1561 atctggccga caaggacgag gtgtccttcg tggtcacctt caagctgctg aaggtcgtgg
     1621 gcggcaccga catcgagtgg gtcaagcccc ccatcaccta cagcatcagc tgctccatcg
     1681 gcaagaacgc cggcatcgac cactgggaga caatcgtgac caccatcagc ggcgagagca
     1741 gctacaccaa gttcagcgac ttcgtgcggt gccggaagat caagttcgtg atggtcccca
     1801 acgatttcgt ggccatccgc agcgtcagat ttgtgggcga gcccgacgcc ctgttccgga
     1861 tccagagcgg catcaccagc gtgcaggaca tgtgcctcca ggtggaccag accggggagc
     1921 tgatcctgtc cagctgcatc atccctatcg ccatgaacag cggcctggac atcttcaagt
     1981 tcaacagcaa cggccagctg tacagcccca tcctggataa gtgcgtggtg gtcaagagca
     2041 gcaacgacat catcgacagc accaacaacg gcatgggcga actggtcatg atgggcgact
     2101 gcaccgagta cctggacaag ggcggcagca tctggcacct gaccccccag aaccagctgt
     2161 ccgtggataa gagcggcgac tactgcctga gccagagagg cagcaaggcc ggctacaacg
     2221 atgtggccat gagcaagctg gtgtctagct ccgacgatag cgacgagtac cccccctaca
     2281 atgctgtgga cggcaacccc gccaccaagt ggcagagcag acccttcgcc accgagaagc
     2341 cctccatcgt gacactgacc ctggacctga tggaagccta caaggtggaa cgggtggaaa
     2401 tcctgtggga cagcgcccca ctgacctacg agatcctgat cagcaacgtg ggcgacggcg
     2461 acaacggcgc caagggcgat aatggggcta agggggacaa tggcgtgcgg tacaagttcg
     2521 agtccgtggt ggaagtgaag tccaacatcc tggtgcacac ccacaacgag atcggcggca
     2581 tctcctgcca gtttgtgaag atcgtgatga agaacggcct gagcgagcct agcaacagcc
     2641 agtacagctg ctacggcatc agaagcgtgg gcatcctggc caaccggctg acaaccatcg
     2701 tggaagagtg cagcattgcc aagaagtcct acgacagccg ggacaagtac ttcctggaat
     2761 ccatccacca ggtcgacctc cacaagctgg acagcgtgcg ctccaagctg aactccctga
     2821 tcggcgatat cgagaacctg gcttctaagc tggaacatct gcccatcgac ctgtgcaagc
     2881 gggatatcct gcagaagtct gagaagatcg agtttctcga gcgcgagttc gaagtgatca
     2941 cccagaatct cgacaagctg cactactcca accccgtgaa gtctaccacc ctgggcatct
     3001 ctagcgacaa ccccggcaga gactgcctgg aaatcaaaaa caacaaccct cagatcccca
     3061 gcggcttcta ctacatccgg cacgagtgcg ccaccagcgc catccgggtg tactgcgaca
     3121 tgaagatggg cctgacatac ctggaacagc agatcgacat tgactccgtg tccgtgaacg
     3181 acgccgtgaa catgtgcaaa gccgtgggcc tggatgtcgt gcggatctac gatcctgccc
     3241 aggtccgagc catccagcac atgctgcagg tcatctccgc cgactccgac gctctgtacc
     3301 ccgtggcctt catcagcgac ggcaagttta agagcctgga tctgaacacc gatagcaccg
     3361 aggccctgga aaatctggtc aaccacaatg acggcgataa cacaatcgcc gtgtcccggg
     3421 acgccatcat gggcatcgat tccaccacca aagagatggc cggaatcatc tgcggctttt
     3481 atggccagcc catgaagagt atcgactgcg tgacaacaat gaagtccctg tcccacacac
     3541 tgggcaacga gtatctgttc agctgcccta gcgactgccc caagaagctg gataccgaca
     3601 agttccggct cgtgggaggc ttcgaggctc tgaccacaaa caccggcgtg cacaaggcct
     3661 tcgtgtttac catggacagc cccctgtgcc tggccgctgc tgtgaatggc gccagatata
     3721 tccggaccaa agtgatccag ggactggaaa gctacggcgg ctttgtggcc aacggggtgg
     3781 tggccgaggc tgtggaaggc cagaggggcg agtttgccgt ggaaattctg ggcctgtgca
     3841 gcaatagcaa gagcgacaat atcctgtcta gcggcaatac cgccatcgga aagaccaaga
     3901 tctccggcac cagcgagatc ccaaaggccg ctccccggaa ggacacaggc atcggcgact
     3961 ccgcctccga tggcaagatg accaactcca acaatgtgca gaccgacaat gagctggact
     4021 ccaccaacac cgccgattac aacatctaca accacgagaa cgatgagtac tacaacaacg
     4081 ccaacaccca gagacagatg ggactgctcg tgcggctgtt cgacaagagc atccaggaaa
     4141 tctccaacag cttcatcaac ctgctgagca cccacgtgaa ccacattctg cacaacacca
     4201 gaagcctgct gatccccaca ctgttcgcct accagcggaa cgacaagaat ctgctgaacc
     4261 tcgagcggaa gtttaccgag atccagtcca agctcgacct ggccaccacc tacctcgtga
     4321 acaaagccaa agagtcccac gatcagtacc ggaccgtgaa gcagaagtac gataagcacc
     4381 agagctttct gagctggtcc atcaaccagc tccagaactt caacatggaa ttcggctacc
     4441 tgggaatcgg catctgggaa gtcaacgatc agaccatcca gtactttaac gaccagcaga
     4501 ccaacccaca gcagctgaag gaccgcagca cctccatctg cgtgaagaga gacaagaacc
     4561 tcttcgactt catctacgtg gccacagtga agtttgtggg aagcggctct gccggcctgg
     4621 tgttcaggtt ccagaaccct gccaactact atctggtgca gctcgtgcag aatgccgata
     4681 acgacgggga gatgtacaag agactcgtcc gggtgcagga tggaatcgcc cacgagctgg
     4741 cccggttcat caatatcaac gagcccagcc ccgacgacaa ttccaacgag gacgtgacca
     4801 tccggatcga ggccagacgg gaaaagctgg gcgtgtcctt tctgaagcac acctccgata
     4861 tctacagcac cccccccttc gacatcaacg tgatcgacga cggcctgctg tccggcacat
     4921 tcggcttcac cgccagctcc ctgcctagag tgaccttcag caatgtgaac atcgaagccc
     4981 tgaactgcat caacaatatc tatacctccc ccatctccaa gacctgcacc acatacaatg
     5041 tcgaggacat tgtgaccgac gactggatca gcctgccccc tggcaaggtc gaagtgtcca
     5101 atatgcccat ctactccgcc cagtcctccg tgctcgagaa caatctggac aacaagcact
     5161 tcttcggcgg gaacaagatg aagtctctga tcatgcggga cctgggagat aaccggtact
     5221 acctgctggg caatcggaga agctgcctgt tcggagactt ccacttccac atctttctgc
     5281 agtgcaccga cggctacgct ggcggcgtcg tccggttctc caatgccaac aactacatca
     5341 catttctgac ctccgccact aacagcaagc tcatgcaggt gacagacgga atcccttacg
     5401 aactggccac atcctccatc cggctgaaga ttggccggtg gtacaaagtg aagattagca
     5461 tgaccaagcg cgacgatcag ctctacatca gcgtgttcat ggatgagcag tccctggctg
     5521 acgataccac ccacgccaac tctaacgtgt tcagcaagtc caccacaatc acaagcaaga
     5581 acaagcacca gctgtttacc gtgggactga ccaaccgcgg ctgcaaggca gtggccttta
     5641 ccaatatcgc cctcgagaga aactccgcca atggggcctc ctccagcaac aacaaggccc
     5701 tgctggccct gaagctgagc cgcgagggcg ccaatctgct cagcctgtgt ctggaacact
     5761 ccatcaatgc ctgtgccggc gacgtggaat gctgcaagaa ctgttgcatc accgcccagg
     5821 gctactctac caaagccggc aatgagtgcg agtactcctg cgctaacggc gctatgaagt
     5881 catacaacga caacgaggcc gtcggcggcg accggatcta taacaatgcc gagctgggcg
     5941 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     6001 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     6061 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     6121 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     6181 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     6241 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     6301 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     6361 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     6421 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     6481 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     6541 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     6601 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     6661 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     6721 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     6781 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     6841 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     6901 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     6961 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     7021 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     7081 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     7141 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     7201 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     7261 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     7321 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     7381 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     7441 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     7501 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     7561 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     7621 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     7681 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     7741 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     7801 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     7861 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     7921 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     7981 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     8041 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     8101 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     8161 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     8221 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     8281 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     8341 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     8401 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     8461 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     8521 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     8581 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     8641 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     8701 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     8761 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     8821 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     8881 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     8941 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     9001 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9061 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9301 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9361 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     9421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9661 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     9721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10921 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10981 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11041 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11101 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    11161 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
    11221 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
    11281 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
    11341 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
    11401 ttggctcatg tccaatatga ccgc
//
