LOCUS       sequence-213068-        9849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g04335 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g04335-bio-His
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5013..5030
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5082..5137
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5136..5155)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7373..7391)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7459..7563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7564..7632,7633..8424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7782..7801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8595..9183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9084..9103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9337..9354
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9471..9492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9507..9512,9513..9530,9531..9537)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9545..9561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9550..9572
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9569..9585
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9569..9585
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 gcgacatcaa gctggaaatc aacgagatca agaaccggct gctgaacatg ctgcagttcg
     1261 agaacgtggg cgagaacaag cccgagaatg tggcctacaa agtgatctac gccaacaccc
     1321 aggtgcccaa cgacgtgatc aaaaagatca gcaagatcgt gaaccggaac gagcccatcc
     1381 acgacgccct gatccggaac tacgccgaca acgacgccgc cctgagctac ttctggctga
     1441 tggtggaaac cctgttcggc gtggaagcct acaacggccg gtacagaatc aagagcagcg
     1501 gcgaggtggt caacagcctg gtggactacg tgatcctggc cgacaagaac ttcgccttcc
     1561 cactgtacat caaagaagtg aaggacgccg agaagcggat caatgagttc atcatcagca
     1621 gcttcgacca gggaatcgcc cccaacctgg atgagatcat cgaccggatc ggcatcgaga
     1681 tcgtgcagct gctgaccctg ctcgtgaaca gcgtgaacat ggaaggcaag ttcgaagagg
     1741 accagcggct gcccaagagc ttcctgagca agtccttcca gtggcagttc ggcattcacg
     1801 actctgccgc cgacagcaac aacgacaagc tgttcagcaa gctgacctcc ttcgacatct
     1861 tcgccagcga ccacctgagc agcctgagct tccggcccaa ccacatgttt aaagagatcc
     1921 tgtccaccgg cttcaccagc aactttgccg gccctagcaa catcatccag ggccagagcc
     1981 tgaacttcaa ggacgtgtac gatatcttca acatgatctc caacctgaag tacaagaaga
     2041 tgtcctccaa caatatcatc cccctgatca tgcggaacat ctgcctgaaa tactccggca
     2101 agtttggcaa cctgctgagc gagtacctgg aactgcccca gagcagcatc agcatgctcg
     2161 tgggagagat gcactgcgtg cggggctacc tgttcatctt cggcttcccc gactccaagg
     2221 gcaagctgtc cttccccaac ggctggccca gaaacatcga ctacgccctg gctgacatcc
     2281 tgagcggcca ggcctctggc tgcggcctgt gctacgctct gatcggcttt ctgctggcca
     2341 tcggctaccc ccccgtcgtg aacggctaca gcggcatcag cgtgaccagc accggcagca
     2401 ccgtccaggt gctgcccccc ctgtccaatc tgaagatccc cttcgacgcc ccctacaata
     2461 gcttcgagta ccccgaggaa aagagcggct tcgatcagcc cttcatcagc ttcgtgaccg
     2521 gctccaagct gggcgacagc ctgtccatgc tggctcacag ctactacctg aaaaccggct
     2581 ttggccacgt gtccaacgtg tacatgggcc tgcctaagtc cagccactac agcctgcaca
     2641 gacccgccga cgagagcccc gtgaagtgcc tgagagccat cccctacatc ctggaaattg
     2701 cccggaacag catcaacggc gccaacggcg acaattggct gcaccccgat atcaacgatg
     2761 cctacgcctc caccatgatc cagaacgccc acgacatgca caacgccgcc agatattacg
     2821 ccgagtttat caagtctatc gcctccgagg gcaaccccga cggcattaga gccctgggcg
     2881 agctgtacta tatgggccac gacgacggcg agatcagccg ggatatcgtg cgggccctga
     2941 gaatctgggg cgacgctgcc gagagcggcg atcctgtgtc tgccctggcc gtggcctccg
     3001 tgcatctgca gagcggcagc aactccctgt cctccgccga gcccttcctg cggatggtgc
     3061 tgaacgaccc caagtccacc aaagagcaga tctacctggc caagttctac atggccagat
     3121 acggcctggg cagagagaag aacccctacg aggccgggga gcatctgctg aaggccggca
     3181 ccttcggcga caccatgaca cagatgctga tcggacacgc ctatgccgga atcctcgtgg
     3241 gcgtgaagat gcacgagggc accaatctgt accgcgccct gaagcactat aagtatgccg
     3301 ccaacgccgg cagcgtcgtg ggcaagtaca acagcgccat cctgaccatt cagggctacg
     3361 accccctgta caccaccgcc tacgagcggt gcaatgccgg ctatcccctg ttccacagcg
     3421 tggccatgaa gaacaccgcc gtggctagcc tgctgcatat ggccaagcgg gccgagattc
     3481 tgaacgatca gacaggcgcc ctgctgtgtt acatgatgct gtccgaagtg ggccaccccg
     3541 tgggacacgt gcggagcgcc aagctgtgga agtacagaaa ctacgacaac aatttcgccc
     3601 agtccttcgt ggccaatctg gccatggccg tgctgggaaa gttcaccgga agcctgatca
     3661 cctccaatgt gcccctgagc cagaaagagt actacaagtc ctccattaag tacgccattc
     3721 tggccgataa cttcatcttt agcaaggatg ccaactccgc caagaagctg cactatcagc
     3781 acctgctcaa gtccgagtat ctgttctgct ttgccagacc cgtgtcccac taccagcagc
     3841 tgtctaacaa cgaaaacgtg aacctgctcg attacggcaa cgataacaag tgtctgcggg
     3901 gcagggacgg caatgagctg tgcctgttct actacagcaa gagagccgcc gctcggaacg
     3961 atcccgacgc catgagctat atcgccaaca gctacctgac aagcaagaac tggatcaagc
     4021 gggactactc taccgccttc aaatgggcct ccgacgcagc caaactgggc aacggcatgg
     4081 gactgtatat cagcgccaga ctgctggaac ggggcttcgg agtggaccgg aactgcgaca
     4141 tggctatcat gtattacaag accctgtttg agtcccccca cctcgagagc aagattgtgg
     4201 gctttatcat gatctggatg accgtgctca accggttcat ctacaacctg cagctgaaga
     4261 ttggcttcga attcatccag ttcagctaca agccctgcaa cccctatgtg atcgagttca
     4321 agatgagcga caagttcgcc aagatgttcg ccatcgtgac aggcgcgccg tcgacctcca
     4381 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     4441 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     4501 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     4561 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     4621 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     4681 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     4741 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     4801 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     4861 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     4921 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     4981 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     5041 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     5101 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5161 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     5221 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     5281 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     5341 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     5401 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     5461 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     5521 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     5581 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     5641 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     5701 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     5761 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     5821 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     5881 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     5941 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     6001 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     6061 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     6121 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     6181 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     6241 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     6301 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     6361 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     6421 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     6481 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     6541 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     6601 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     6661 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     6721 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     6781 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     6841 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     6901 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     6961 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     7021 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     7081 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     7141 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     7201 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     7261 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     7321 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     7381 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7561 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7621 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7681 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7741 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7801 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7861 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7921 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7981 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8041 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8101 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8161 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8221 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8281 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8341 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8401 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8461 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8521 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8581 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8641 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8701 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8761 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8821 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8881 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8941 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9001 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9061 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9181 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9241 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9301 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9361 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9421 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9481 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9541 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9601 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     9661 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     9721 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     9781 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     9841 tatgaccgc
//
