LOCUS       sequence-213071-        7884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g01540 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g01540-bio-His
FEATURES             Location/Qualifiers
     source          1..7884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3048..3065
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3117..3172
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3171..3190)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5408..5426)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5494..5598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5599..5667,5668..6459)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5817..5836)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6630..7218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7119..7138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7372..7389
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7506..7527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7542..7547,7548..7565,7566..7572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7580..7596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7585..7607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7604..7620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7604..7620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tggtgctgag caatagcaat tccgccggca agggcgtgtc caagtctgag ggcggcgagg
     1261 gcctggacgt ggtggtcaag acaaaccacg ccgcccagag cgacagcacc aacaacgaga
     1321 tcagcgacgg ccggacctcc atcaacgacg agaagaaagt gaacaaagag gacagcagcg
     1381 gcagcgactc cagcgtggaa agctccggca gcggcgagaa agagggcgcc aaagtcggca
     1441 acaaagaaga tgaggaaaag aacgagcaca agtccgatga gaagaatgag cataagagcg
     1501 acgaaaagaa tacccccgtg gacgacaaga acaacgccga gagcggcccc aaagagcaga
     1561 ccacagacgc cgccaccggc gactccgtga agcacagcga gttcgaagag tacaagaaag
     1621 aagaaggcga gaaggtcgac aagctggcca aggacgagca ggccaaatac gaggacctgg
     1681 aagccaaggt ggccaaactg accgagtccg tgaacgtgat cgagaagttc gaggaaaaac
     1741 tggccagcat caagcccgtg acccaggaaa cccagagcaa gacccagcag aaggtgcccg
     1801 gccagcaggg caaggccgag tctgccaaca tcatcacctt cagcaaggcc agcttcaact
     1861 gccccctgga ctgcaccggc tgcagcaacg gcgtgggcaa gaacttcttc tgcaagggca
     1921 ccatctgcaa gccctacaag aacctggccg acttcagctg cgagcagggc ctgaccatgt
     1981 gcggcgtgtt caacgtgaac gacaaccagg ccttcaacct gatcagcagc aacagcgcca
     2041 gccggaccct gcagagcttc gaggtgcagg gctccaacat gaacccctgc gtggaactgc
     2101 tggtggtgcc caacagctac gtgtgcgacc ggatgaacat caacgtgctg gaacaggaat
     2161 ctcagaagat cctggacccc aacttcatct ccaccatctt ccccaccggc atcagattcg
     2221 acaacctgct gatcaatgcc gcccctggca actacaaagt gtgcatggcc cagtacagca
     2281 acgacgcccg gctggccggc agctttgtgt ccagcctggg caacctgttc aacagactga
     2341 gcaacccccc accccagctg atctccatcc agcaggccgg cacaatcatc ctgaccggcg
     2401 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2461 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2521 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2581 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2641 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2701 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2761 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2821 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2881 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2941 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     3001 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     3061 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     3121 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3181 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     3241 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     3301 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     3361 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3421 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3481 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     3541 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     3601 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     3661 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     3721 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3781 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3841 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3901 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     3961 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     4021 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     4081 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     4141 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     4201 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     4261 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     4321 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     4381 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4441 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4501 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     4561 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     4621 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     4681 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     4741 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4801 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4861 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4921 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4981 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     5041 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     5101 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     5161 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5221 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     5281 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     5341 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     5401 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5461 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5521 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5581 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5641 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5701 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5761 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5821 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5881 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5941 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6001 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6061 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6121 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     6181 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6241 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6301 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6361 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6421 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7321 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7381 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7501 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7561 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7621 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     7681 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     7741 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7801 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7861 ttggctcatg tccaatatga ccgc
//
