LOCUS       sequence-213074-         407 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Delivery of human-codon-optimized Cas9 from Neisseria meningitidis 
            (NmeCas9) and its single-guide RNA in a single AAV vector for in 
            vivo genome editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Ibraheim R, Song CQ, Mir A, Amrani N, Xue W, Sontheimer EJ
  TITLE     All-in-one adeno-associated virus delivery and genome editing by 
            Neisseria meningitidis Cas9 in vivo.
  JOURNAL   Genome Biol. 2018 Sep 19;19(1):137. doi: 10.1186/s13059-018-1515-0.
  PUBMED    30231914
REFERENCE   2  (bases 1 to 407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-09-19"; volume: "19(1):137. doi"; pages: " 
            10.1186/s13059-018-1515-0"
COMMENT     Sequence Label: pEJS654 All-in-One AAV-sgRNA-hNmeCas9
FEATURES             Location/Qualifiers
     source          1..407
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(16..108)
                     /label=Nm tracrRNA
                     /note="trans-activating CRISPR RNA for the Neisseria 
                     meningitidis CRISPR/Cas9 system (Hou et al., 2013)"
     promoter        complement(159..407)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(217..236)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(387..407)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtttaaacaa aaaaataaac gatgcccctt aaagcagaag ctttaagggg cagagcgttg
       61 cggcacatct tttcagacgg ccttattgta gcaacggttc tcatttcgtt tccgaaatga
      121 gaaagggagc tacaactgaa gagctggaat gctcttccgg tgtttcgtcc tttccacaag
      181 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
      241 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
      301 ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc
      361 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctc
//
