LOCUS       sequence-213082-        5514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5514)
  TITLE     Johannes Schmid plasmids
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4xNFkB Luc
FEATURES             Location/Qualifiers
     source          1..5514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1909..1926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1941..2081
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2007..2026)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2320..2441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2572..2603
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2630..4282
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2696..2714)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4413..4478
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4608..4628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5053..5187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5460..5478)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1861 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1921 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     1981 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2041 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     2101 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2161 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     2221 aggatcagcc atatcacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2281 cccctgaacc tgaaacataa aatgaatgca attgttgtta acttgtttat tgcagcttat
     2341 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg ggccctggga
     2461 ctttcctctg ctgagaaact ttctgctggg actttcctct gtctccgccc tgggactttc
     2521 ctctgctgag aaactttctg ctgggacttt cctctgtctc cgcggagact ctagagggta
     2581 tataatggaa gctcgaattc cagcttggca ttccggtact gttggtaaaa tggaagacgc
     2641 caaaaacata aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga
     2701 gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga
     2761 tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc
     2821 agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa
     2881 ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc
     2941 cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac
     3001 cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc
     3061 aataatccag aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat
     3121 gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga
     3181 gtcctttgat cgtgacaaaa caattgcact gataatgaat tcctctggat ctactgggtt
     3241 acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga
     3301 tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca
     3361 tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt
     3421 aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag
     3481 tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata
     3541 cgatttatct aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg
     3601 ggaagcggtt gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga
     3661 gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa
     3721 agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt
     3781 taatcagaga ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa
     3841 tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc
     3901 ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata
     3961 caaaggatat caggtggccc ccgctgaatt ggaatcgata ttgttacaac accccaacat
     4021 cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt
     4081 tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag
     4141 tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa
     4201 aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa
     4261 gggcggaaag tccaaattgt aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta
     4321 ttgtaatact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     4381 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     4441 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     4501 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     4561 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     4621 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     4681 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     4741 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     4801 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     4861 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     4921 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     4981 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5041 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5101 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5161 atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
     5221 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
     5281 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
     5341 tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
     5401 ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
     5461 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt ctta
//
