LOCUS       sequence-213103-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-let-7a-5p
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(104..128)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(352..371)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    417..472
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(471..490)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gaggccgaca aagagacata cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
       61 ctccctagca aactggggca caaacttaat tgagaattct accacccagc tttcttgtac
      121 aaagtggtac gtctcaagac cagaggtctc tgctttgaat gctaactata caacctacta
      181 cctcaaacta tacaacctac tacctcaaac tatacaacct actacctcaa actatacaac
      241 ctactacctc acaaccagaa gagacctgtg cttcactcct caggtgcagg ctgcctatca
      301 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
      361 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
      421 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
      481 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
      541 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
      601 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
      661 gatttttt
//
