LOCUS       sequence-213111-        5922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 
            / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5922)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-TES-N-14
FEATURES             Location/Qualifiers
     source          1..5922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1962..2642
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2764..2885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2801..2820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2855..2874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2892..3347)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3029..3050)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3241..3260
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3374..3478
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3480..3837
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3480..3671
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3503..3523)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3688..3823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3872..4666
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3872..4666
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3926..3945)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4536..4555
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4854..4873)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4898..4945
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5274..5862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5763..5782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gccaccatgg
      661 acctggaaaa caaagtgaag aagatgggct taggtcacga gcaaggattt ggagcccctt
      721 gtttaaaatg caaagaaaaa tgtgaaggat tcgaactgca cttctggaga aaaatatgtc
      781 gtaactgcaa gtgtggccaa gaagagcatg atgtcctctt gagcaatgaa gaggatcgaa
      841 aagtgggaaa actttttgaa gacaccaagt ataccactct gattgcaaaa ctaaagtcag
      901 atggaattcc catgtataaa cgcaatgtta tgatattgac gaatccagtt gctgccaaga
      961 agaatgtctc catcaataca gttacctatg agtgggctcc tcctgtccag aatcaagcat
     1021 tggccaggca gtacatgcag atgctaccca aggaaaagca gccagtagca ggctcagagg
     1081 gggcacagta ccggaagaag cagctggcaa agcagctccc tgcacatgac caggaccctt
     1141 caaagtgcca tgagttgtct cccagagagg tgaaggagat ggagcagttt gtgaagaaat
     1201 ataagagcga agctctggga gtaggagatg tcaaacttcc ctgtgagatg gatgcccaag
     1261 gccccaaaca aatgaacatt cctggagggg atagaagcac cccagcagca gtgggggcca
     1321 tggaggacaa atctgctgag cacaaaagaa ctcaatattc ctgctattgc tgcaaactga
     1381 gtatgaaaga aggtgaccca gccatctatg ccgaaagggc tggctatgat aaactgtggc
     1441 acccagcttg ttttgtctgc agcacctgcc atgaactcct ggttgacatg atttattttt
     1501 ggaagaatga gaagctatac tgtggcagac attactgtga cagcgagaaa ccccgatgtg
     1561 ctggctgtga cgagctgata ttcagcaatg agtataccca ggcagaaaac cagaattggc
     1621 acctgaaaca cttctgctgc tttgactgtg atagcattct agctggggag atatacgtga
     1681 tggtcaatga caagcccgtg tgcaagccct gctatgtgaa gaatcacgct gtggtgtgtc
     1741 aaggatgcca caatgccatc gacccagaag tgcagcgggt gacctataac aatttcagct
     1801 ggcatgcatc cacagagtgc tttctgtgct cttgctgcag caaatgcctc attgggcaga
     1861 agttcatgcc agtagaaggg atggttttct gttcagtgga atgtaagaag aggatgtctt
     1921 ccggcggtag cggcggtagc ggggatccac cggtcgccac catgagtgcg attaagccag
     1981 acatgaagat caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg
     2041 gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg
     2101 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg
     2161 tattcgccaa atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt
     2221 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcaacgcc agaaacgaca
     2281 taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg
     2341 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt
     2401 atgtgcgtga tggagtgctg acgggtgata ttgagatggc tttgttgctt gaaggaaatg
     2461 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac
     2521 caggcgccca ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca
     2581 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgat
     2641 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2701 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2761 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2881 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2941 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     3001 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3061 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3121 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3181 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3241 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3301 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3481 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3541 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3601 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3661 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3721 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     3841 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     3901 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3961 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4021 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4081 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4141 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4201 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4261 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4321 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4381 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4441 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4501 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4561 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4621 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4681 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     4741 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4801 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     4861 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     4921 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     4981 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     5041 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     5101 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     5161 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5221 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5281 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5341 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5401 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5461 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5521 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5581 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5641 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5701 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5761 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5821 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5881 tttttacggt tcctggcctt ttgctggcct tttgctcaca tg
//
