LOCUS       sequence-213118-        5487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 507 / 572, 
            Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5487)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MCPB2-N-18
FEATURES             Location/Qualifiers
     source          1..5487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1527..2207
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2329..2450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2366..2385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2420..2439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2457..2912)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2594..2615)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2806..2825
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2939..3043
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3045..3402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3045..3236
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3068..3088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3253..3388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3437..4231
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3437..4231
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3491..3510)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4101..4120
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4419..4438)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4463..4510
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4839..5427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5328..5347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gccaccatga
      661 gcgatcagca gctggactgc gccttggacc tgatgaggcg cctgcctcca cagcagattg
      721 agaagaacct cagcgatctg atcgacctgg tccccagtct gtgtgaagat ctcctgtcat
      781 ctgttgacca gcccctgaaa attgccagag acaaggtggt gggcaaggat taccttttgt
      841 gtgactacaa cagagacggg gactcctata ggtcaccgtg gagtaacaag tatgaccctc
      901 ctttggaaga tggggccatg ccatctgctc ggctcagaaa gctggaggta gaggccaaca
      961 atgccttcga ccaataccga gacctgtatt ttgaaggtgg ggtctcatca gtctacctct
     1021 gggatcttga tcatggcttt gctggagtga tcctcataaa gaaagctgga gatggatcca
     1081 agaagatcaa aggctgctgg gattccatcc acgtggtgga agtgcaggag aagtccagcg
     1141 gccgtactgc ccattacaag ttgacctcca cggtgatgct atggctgcaa accaacaaat
     1201 ccggctcggg caccatgaac ctgggaggca gcctaaccag acagatggag aaagacgaaa
     1261 ctgtgagtga ctgttcccca cacatagcca acatcgggcg cctggtggag gacatggaaa
     1321 acaaaatccg aagcacgctg aatgagatct actttggaaa aacaaaggac atcgtcaacg
     1381 ggctgaggtc tgtgcagacg tttgcagaca aatcaaagca agaagcgctt aagaacgacc
     1441 tggtggaggc cttgaagaga aagcagcagt gtagtcgcgg aggcagtggt ggggtaccgc
     1501 gggcccggga tccaccggtc gccaccatga gtgcgattaa gccagacatg aagatcaaac
     1561 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
     1621 agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt
     1681 ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc gccaaatatc
     1741 cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
     1801 gcttgacttt cgaagacggg ggcatttgca acgccagaaa cgacataaca atggaagggg
     1861 acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta
     1921 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
     1981 tgctgacggg tgatattgag atggctttgt tgcttgaagg aaatgcccat taccgatgtg
     2041 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc gcccactttg
     2101 tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
     2161 agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc
     2221 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2281 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2341 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2401 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2461 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2521 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2581 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2641 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2701 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2761 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2821 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2881 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     2941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3061 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3121 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3301 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3361 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3421 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3481 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3541 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3601 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3661 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3721 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3781 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3841 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3901 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3961 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4021 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4081 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4141 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4201 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4261 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4321 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4381 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4441 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4501 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4561 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4621 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4681 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4741 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4921 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4981 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5041 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5101 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5161 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5221 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5281 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5341 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5401 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5461 gccttttgct ggccttttgc tcacatg
//
