LOCUS       sequence-213120-        5533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 507 / 572, 
            Emission: 516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5533)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MCPB2-C-18
FEATURES             Location/Qualifiers
     source          1..5533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             671..1348
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2379..2500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2416..2435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2470..2489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2507..2962)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2644..2665)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2856..2875
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2989..3093
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3095..3452
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3095..3286
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3118..3138)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3303..3438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3487..4281
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3487..4281
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3541..3560)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4151..4170
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4469..4488)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4513..4560
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4889..5477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5378..5397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggtcgccacc atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa
      721 cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa
      781 acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct
      841 gaccactgca ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga
      901 ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga
      961 cgggggcatt tgcaacgcca gaaacgacat aacaatggaa ggggacactt tctataataa
     1021 agttcgattt tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct
     1081 gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat
     1141 tgagatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta
     1201 caaagctaag gagaagggtg tcaagttacc aggcgcccac tttgtggacc actgcattga
     1261 gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca
     1321 ttctggattg cctgacaatg ccagacgatc cggactcaga tctcgagagt ccagtggata
     1381 ttcgtctcgg tcgggtggaa gcagcgatca gcagctggac tgcgccttgg acctgatgag
     1441 gcgcctgcct ccacagcaga ttgagaagaa cctcagcgat ctgatcgacc tggtccccag
     1501 tctgtgtgaa gatctcctgt catctgttga ccagcccctg aaaattgcca gagacaaggt
     1561 ggtgggcaag gattaccttt tgtgtgacta caacagagac ggggactcct ataggtcacc
     1621 gtggagtaac aagtatgacc ctcctttgga agatggggcc atgccatctg ctcggctcag
     1681 aaagctggag gtagaggcca acaatgcctt cgaccaatac cgagacctgt attttgaagg
     1741 tggggtctca tcagtctacc tctgggatct tgatcatggc tttgctggag tgatcctcat
     1801 aaagaaagct ggagatggat ccaagaagat caaaggctgc tgggattcca tccacgtggt
     1861 ggaagtgcag gagaagtcca gcggccgtac tgcccattac aagttgacct ccacggtgat
     1921 gctatggctg caaaccaaca aatccggctc gggcaccatg aacctgggag gcagcctaac
     1981 cagacagatg gagaaagacg aaactgtgag tgactgttcc ccacacatag ccaacatcgg
     2041 gcgcctggtg gaggacatgg aaaacaaaat ccgaagcacg ctgaatgaga tctactttgg
     2101 aaaaacaaag gacatcgtca acgggctgag gtctgtgcag acgtttgcag acaaatcaaa
     2161 gcaagaagcg cttaagaacg acctggtgga ggccttgaag agaaagcagc agtgttgaga
     2221 attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa
     2281 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2341 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2461 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
     2521 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2581 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2641 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2701 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     2761 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     2821 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     2881 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     2941 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3001 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3061 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     3121 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3181 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3241 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3301 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3361 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3421 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     3481 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3541 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3601 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3661 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3721 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3781 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3841 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3901 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3961 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4021 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4081 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4141 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4201 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4261 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4321 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4381 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4441 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4501 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     4561 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4621 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4681 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     4741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5521 ggccttttgc tca
//
