LOCUS       sequence-213140-        8334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_02g04075 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_02g04075-bio-His
FEATURES             Location/Qualifiers
     source          1..8334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3498..3515
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3567..3622
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3621..3640)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5858..5876)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5944..6048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6049..6117,6118..6909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6267..6286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7080..7668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7569..7588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7822..7839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7956..7977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7992..7997,7998..8015,8016..8022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8030..8046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8035..8057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8054..8070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8054..8070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 agtgcgtgca gctgaagtgc ctgttcttca agcccacctt cgtgtccatc agcctgaacg
     1261 gcctgaagaa cacccggctg aaccgggtgt ccctgatgtt cgagggcaac gaccggcacg
     1321 acctgctgcg gcacctgtac atcaacagag agaagatctt ccgggacaaa gaggtggccg
     1381 agcacctgaa aaactacttc ggcctgaccc tggaaaacct ggaacagcac gtgatcgaca
     1441 ccatggaaga acagatccgg gaccgggacg ccacccggga cgagaagcct atctgggtgg
     1501 acgccaaggt gttcaagggc gctcccgaga agttcaagct gcccctggac cccaccctga
     1561 gcctgctgaa gggcgagggc atccaggaca tcaaagaagt ggaaggccag gaattcatca
     1621 gcgacttcga caagaccatc tacgagctgc tgcagaacgc cagagccaac tgcgtgaagc
     1681 ggatccccca gaccgagctg gaaaagttca tcgagctgta cagcaaagtg ggcagctacg
     1741 agtacgagat cgtggacaac gaactgtact actacaccga ctgccagccc ctgaagagaa
     1801 tcaagctgac cgaccagcac gagaagagac agcaccagat tgagctgatg agccggccca
     1861 cccggaaccc cgccttcatc gaccccgtgg accctcacat ccggaccggc agacagcggg
     1921 ccctgcagcg gaaaagagac tccggcgctc tgcagaaaaa gcggtggaag atggaagtgc
     1981 cccccgaaga ggacatcccc agaggcaaga gcatccgggg ccaccccatc atccagaaat
     2041 tcgtggactc catggacttc gagcggcagc tgagcaacgc ccaggacacc aattacttca
     2101 agagcaactt cctggaaacc ggccacaaca tccccgaccg ggccatggac agctacttcg
     2161 atatccagtt tatcggcacc cccgacgcct accctgccgc caaagaaccc gacatgacct
     2221 tcacctggcc cctgtactgg gtgccctggc tggccaagaa gcaccccaag cggagaggcc
     2281 agcctatcaa gagccccgtg ttcaacctgg gcggcatcga gcctctgcag ctgtggttct
     2341 atccacaggg caccgccatg agcaagcccg gctacagcag catgaagctg atcacccctc
     2401 ccggctggaa tctgccctac cggatctacc tgtatatctt cagcgagtac aacaaggtgg
     2461 tgtccaaggc catgtacaaa gagagcgtcg actatatcgc cttcaccctg aacagcgcca
     2521 gactgctgac caagcggggc atcgacgatc tgctggaaag ccccaaggac aatgacgact
     2581 acgtgatcct gggcccagcc ggcaacgtgt acgccggcgt gggaatcgcc gacgagcccc
     2641 tgcccgagac agacttcaga ggcgccaagc ggctgcccaa cgtcgagtat aagtacaact
     2701 acgattggca cgacggccac taccacatgc acaagtggct gctgaaacag accgccaacc
     2761 ccgaggacag cgagatcatg gataagctgc ctcggtacta cacacacagc ctggactacc
     2821 agcccttcaa gtactattac acacccggcg cgccgtcgac ctccatcacg gcctataaga
     2881 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     2941 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     3001 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     3061 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     3121 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     3181 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     3241 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     3301 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     3361 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     3421 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     3481 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     3541 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     3601 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     3661 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     3721 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     3781 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     3841 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     3901 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     3961 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     4021 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     4081 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     4141 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     4201 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     4261 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4321 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4381 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4441 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     4501 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     4561 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     4621 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     4681 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     4741 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     4801 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     4861 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     4921 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     4981 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     5041 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     5101 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     5161 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     5221 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5281 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5341 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5401 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5461 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     5521 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     5581 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     5641 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     5701 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5761 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     5821 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     5881 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5941 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6001 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6061 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6241 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6301 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6361 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6421 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6481 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6541 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6601 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6661 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6721 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6781 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6841 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6901 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7741 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7801 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7861 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7921 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7981 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8041 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     8101 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     8161 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     8221 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     8281 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
