LOCUS       sequence-213141-        7381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: mEos2-Alpha-Actinin-C-14
FEATURES             Location/Qualifiers
     source          1..7381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3805..3926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3842..3861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3896..3915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3933..4388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4070..4091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4282..4301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4415..4519
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4521..4878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4521..4712
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4544..4564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4729..4864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4913..5707
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4913..5707
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4967..4986)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5577..5596
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5895..5914)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5939..5986
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6315..6903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6804..6823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7078..7381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg
      301 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
      361 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
      421 ccactgcatt ccattacggc aacagggtat tcgccaaata tccagacaac atacaagact
      481 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
      541 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
      601 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
      661 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc
      721 atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
      781 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
      841 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
      901 ctggattgcc tgacaatgcc agacgatccg gactcagatc tcgaggaagc ggcggatctg
      961 ggtccggaga ccattatgat tctcagcaaa ccaacgatta catgcagcca gaagaggact
     1021 gggaccggga cctgctcctg gacccggcct gggagaagca gcagagaaag acattcacgg
     1081 catggtgtaa ctcccacctc cggaaggcgg ggacacagat cgagaacatc gaagaggact
     1141 tccgggatgg cctgaagctc atgctgctgc tggaggtcat ctcaggtgaa cgcttggcca
     1201 agccagagcg aggcaagatg agagtgcaca agatctccaa cgtcaacaag gccctggatt
     1261 tcatagccag caaaggcgtc aaactggtgt ccatcggagc cgaagaaatc gtggatggga
     1321 atgtgaagat gaccctgggc atgatctgga ccatcatcct gcgctttgcc atccaggaca
     1381 tctccgtgga agagacttca gccaaggaag ggctgctcct gtggtgtcag agaaagacag
     1441 ccccttacaa aaatgtcaac atccagaact tccacataag ctggaaggat ggcctcggct
     1501 tctgtgcttt gatccaccga caccggcccg agctgattga ctacgggaag ctgcggaagg
     1561 atgatccact cacaaatctg aatacggctt ttgacgtggc agagaagtac ctggacatcc
     1621 ccaagatgct ggatgccgaa gacatcgttg gaactgcccg accggatgag aaagccatca
     1681 tgacttacgt gtctagcttc taccacgcct tctctggagc ccagaaggcg gagacagcag
     1741 ccaatcgcat ctgcaaggtg ttggccgtca accaggagaa cgagcagctt atggaagact
     1801 acgagaagct ggccagtgat ctgttggagt ggatccgccg cacaatcccg tggctggaga
     1861 accgggtgcc cgagaacacc atgcatgcca tgcaacagaa gctggaggac ttccgggact
     1921 accggcgcct gcacaagccg cccaaggtgc aggagaagtg ccagctggag atcaacttca
     1981 acacgctgca gaccaagctg cggctcagca accggcctgc cttcatgccc tctgagggca
     2041 ggatggtctc ggacatcaac aatgcctggg gctgcctgga gcaggtggag aagggctatg
     2101 aggagtggtt gctgaatgag atccggaggc tggagcgact ggaccacctg gcagagaagt
     2161 tccggcagaa ggcctccatc cacgaggcct ggactgacgg caaagaggcc atgctgcgac
     2221 agaaggacta tgagaccgcc accctctcgg agatcaaggc cttgctcaag aagcatgagg
     2281 ccttcgagag tgacctggct gcccaccagg accgtgtgga gcagattgcc gccatcgcac
     2341 aggagctcaa tgagctggac tattatgact cacccagtgt caacgcccgt tgccaaaaga
     2401 tctgtgacca gtgggacaat ctgggggccc taactcagaa gcgaagggaa gctctggagc
     2461 ggaccgagaa actgctggag accattgacc agctgtactt ggagtatgcc aagcgggctg
     2521 cacccttcaa caactggatg gagggggcca tggaggacct gcaggacacc ttcattgtgc
     2581 acaccattga ggagatccag ggactgacca cagcccatga gcagttcaag gccaccctcc
     2641 ctgatgccga caaggagcgc ctggccatcc tgggcatcca caatgaggtg tccaagattg
     2701 tccagaccta ccacgtcaat atggcgggca ccaaccccta cacaaccatc acgcctcagg
     2761 agatcaatgg caaatgggac cacgtgcggc agctggtgcc tcggagggac caagctctga
     2821 cggaggagca tgcccgacag cagcacaatg agaggctacg caagcagttt ggagcccagg
     2881 ccaatgtcat cgggccctgg atccagacca agatggagga gatcgggagg atctccattg
     2941 agatgcatgg gaccctggag gaccagctca gccacctgcg gcagtatgag aagagcatcg
     3001 tcaactacaa gccaaagatt gatcagctgg agggcgacca ccagctcatc caggaggcgc
     3061 tcatcttcga caacaagcac accaactaca ccatggagca catccgtgtg ggctgggagc
     3121 agctgctcac caccatcgcc aggaccatca atgaggtaga gaaccagatc ctgacccggg
     3181 atgccaaggg catcagccag gagcagatga atgagttccg ggcctccttc aaccactttg
     3241 accgggatca ctccggcaca ctgggtcccg aggagttcaa agcctgcctc atcagcttgg
     3301 gttatgatat tggcaacgac ccccagggag aagcagaatt tgcccgcatc atgagcattg
     3361 tggaccccaa ccgcctgggg gtagtgacat tccaggcctt cattgacttc atgtcccgcg
     3421 agacagccga cacagataca gcagaccaag tcatggcttc cttcaagatc ctggctgggg
     3481 acaagaacta cattaccatg gacgagctgc gccgcgagct gccacccgac caggctgagt
     3541 actgcatcgc gcggatggcc ccctacaccg gccccgactc cgtgccaggt gctctggact
     3601 acatgtcctt ctccacggcg ctgtacggcg agagtgacct ctaagaattc tgcagtcgac
     3661 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca
     3721 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3781 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3841 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3901 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3961 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4021 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4081 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4141 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4201 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4261 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4321 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4381 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4501 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4561 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4621 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4681 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4801 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4861 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4921 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4981 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5041 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     5101 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5161 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5221 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5281 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5341 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5401 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5461 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5521 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5581 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5641 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5701 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5761 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5821 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5881 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5941 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     6001 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     6061 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     6121 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     6181 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6241 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6301 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6361 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6421 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6481 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6541 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6601 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6661 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6721 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6781 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6841 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6901 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6961 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag
     7021 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     7081 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     7141 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     7201 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     7261 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     7321 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     7381 g
//
