LOCUS       sequence-213147-        9264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_02g00320 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_02g00320-bio-His
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4428..4445
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4497..4552
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4551..4570)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6788..6806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6874..6978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6979..7047,7048..7839)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7197..7216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8010..8598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8499..8518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8752..8769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8886..8907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8922..8927,8928..8945,8946..8952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8960..8976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8965..8987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8984..9000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8984..9000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gacccgccaa cagcaacgac tacctgacca ccagcagcag cgacagcgac tccagcgaga
     1261 gcgagtccga gctggacggc ctgagcgacc tggaagatgc cagcgacgcc gagttcgacg
     1321 acatctacaa cgtggaagat ctgaccagca atgtggacgg caacctggcc aagttcggcc
     1381 acttcgacgg caaggacacc aagaacgagg acacctccgc cagcctggac ctgaacaagt
     1441 ggaacaacgc cgctcggaag aaaagcggca acaaagtgaa gttcagcagc aacaaggcca
     1501 agagcaccac aaagaagaag cggaaaaaga cctacagcac caacgagaac gggaacaagc
     1561 tgaagttcga ggacaaaaag cacgtcgagc acaagaagct gagcagcaag aaaaagctga
     1621 agcagcagca gctgaacgag cagcacggca tcaagcccat caatgccgcc aacgatgccg
     1681 acatgaagca cggactgaag tccggcaaga gcagctccaa gcacgggatt aaggcccaca
     1741 ccttcgacag ccagacacac atcatcacca accagatcgg caccgatcag atccagctgc
     1801 gggactccga gatcgagaac aaggtgtaca agaaactggt ggccgagctg aacaaagccc
     1861 ccgatctgga tatcgccggc atctccgaga gcatcagata cctgggcagc ggctacgaca
     1921 tcatcttcgg caaccccatc ggcgaccccc tgatgatggt ggaccccggc taccggaacc
     1981 ctatcattca gctggaatgg gacaagcaca gcaccttcgc ccacaacaat gccctgcccc
     2041 agcccgtgga tggctgggtc cgacccgaga tcagctgcaa gcaggccgag aaggtggacc
     2101 acgtgaacac cctcgaggac tataagaaag aactgagcgt ggacgccgtg gccagctccg
     2161 acttcctgaa cttcttcgcc tttagcgcca gcggcggcta caagaacttc gccaagctgg
     2221 tcacccagga aaagaccaga agcttcatcc tgaaaaccta ctgcctgcgg tacattgccg
     2281 gcctggaagt gtccaccaac ctgaagctga cccccgcctt caagaatgcc gtggacaagc
     2341 tgcccgtgat cttcgatggc ttcgaagagt acagctcctg ccctatcgag aagtataagg
     2401 ccaatgaggc cgacaccgac tgcgagagca acgtgcggcc ctggatggac ttcttcaaag
     2461 agttcggcac ccacttcacc agcgtggtgc acctgggcgg caagatgacc taccaggtgc
     2521 agatgaagca gtccgacatc aacaaactgc aggaacaggg cgtcaacgtc gacgccgcca
     2581 tcaaggccac ctgtggcttc ggcatgccca atatcgctgg caagatctcc accaagggcg
     2641 agtccacctc catcagcaag atgtccgatt acaaggtcga gaagatgatc atggtcatcg
     2701 gcggcgagcc ccccaaggat ctgaacgacg ccagcaatct gaacaactgg gccaagtccg
     2761 tggctaagag ccccatgcct atcaagagcg agctgatccc catcagagag ctgttcgacc
     2821 tgcacgagct gcagcagagc tacaaccagg ccattaagta ctacagcgag gtgtacggaa
     2881 tcagccagaa cgatatgtac gagatcaagg gcaagattaa gggcatccct gagatcatca
     2941 aagaggccca gcaggtcaca tacgacggcc cagcccctgg cagagtcgtg tgccccattg
     3001 gcaccaccat cctgttcggc ttcagcatga ccattaccaa gaagaaaaaa gccctggact
     3061 ttctgaagaa caccgcctac aatgtgaaca tcgtgccctg cgtcgtgggc aatgagaagt
     3121 gcagcggcac cgcccagagc gatagcatcg tgaagatttg ggccctgtgc agcccccatc
     3181 ccgcccctct gctggtccag gtgtccagcc acaagaacaa cggccctgcc accgtggaat
     3241 gccccaaggg cttcgtgatc ggcatgggct ttggcatcac cttccctaag ggcctgccaa
     3301 tcatgcccca ggatatctac ccctgccgga acggccagac ctcctgcacc aagatccccg
     3361 acaaggacgg cagcaccacc gtgtgggccg tgtgctttga gagcggcgcc atcgaggtgg
     3421 acaatctggc caacaacgct aaggccggct ccagcgcctc ctgcgaggcc cacgatcaca
     3481 tcacctccgg cctcgtgaat acctgccccg acgactacga ggtgctgtgc ggcttctcca
     3541 tggccctgac ccggaagaac agccacaccg acgaccactt caaggcctgc agaggcggca
     3601 aaagctgtgc catcgataag cacaagcgcc acgacaacga gtgtatcgcc tacgcctctt
     3661 ggaacgtgtg ctccctggtg gtgcccggcc acaaggatac cgtgcacccc gccagagcca
     3721 acgtggcact ggaccggacc aagaccgcca atggcatcga gcctatcggc aagaacggcg
     3781 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     3841 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     3901 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     3961 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     4021 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     4081 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     4141 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     4201 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     4261 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     4321 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     4381 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     4441 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     4501 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     4561 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     4621 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     4681 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     4741 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     4801 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     4861 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     4921 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     4981 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     5041 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     5101 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     5161 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     5221 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     5281 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     5341 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     5401 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     5461 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     5521 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     5581 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     5641 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     5701 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     5761 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     5821 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     5881 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     5941 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     6001 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     6061 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     6121 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     6181 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     6241 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     6301 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     6361 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     6421 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     6481 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     6541 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6601 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     6661 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     6721 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     6781 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6841 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7201 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     7261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7501 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     7561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8761 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8821 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8881 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8941 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9001 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     9061 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     9121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     9181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     9241 ttggctcatg tccaatatga ccgc
//
