LOCUS       sequence-213148-        6269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-C-Src-7
FEATURES             Location/Qualifiers
     source          1..6269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             67..108
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1687..2367
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2489..2610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2526..2545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2580..2599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2617..3072)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2754..2775)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2966..2985
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3099..3203
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3205..3562
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3205..3396
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3228..3248)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3413..3548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3597..4391
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3597..4391
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3651..3670)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4261..4280
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4579..4598)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4623..4670
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4999..5587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5488..5507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5762..6065
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6066..6269
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6220..6240
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc
       61 gaattcatgg ggagcagcaa gagcaagccc aaggacccca gccagcgccg gcgcagcctg
      121 gagccacccg acagcaccca ccacggggga ttcccagcct cgcagacccc caacaagaca
      181 gcagcccccg acacgcaccg cacccccagc cgctcctttg ggaccgtggc caccgagccc
      241 aagctcttcg ggggcttcaa cacttctgac accgtcacgt cgccgcagcg tgccggggca
      301 ctggctggcg gcgtcaccac tttcgtggct ctctatgact acgagtcccg gactgaaacg
      361 gacttgtcct tcaagaaagg agaacgcctg cagattgtca acaacacgga aggtgactgg
      421 tggctggctc attccctcac tacaggacag acgggctaca tccccagtaa ctatgtcgcg
      481 ccctcagact ccatccaggc tgaagagtgg tactttggga agatcactcg tcgggagtcc
      541 gagcggctgc tgctcaaccc cgaaaacccc cggggaacct tcttggtccg ggagagcgag
      601 acgacaaaag gtgcctattg cctctccgtt tctgactttg acaacgccaa ggggctcaat
      661 gtgaagcact acaagatccg caagctggac agcggcggct tctacatcac ctcacgcaca
      721 cagttcagca gcctgcagca gctggtggcc tactactcca aacatgctga tggcttgtgc
      781 caccgcctga ccaacgtctg ccccacgtcc aagccccaga cccagggact cgccaaggac
      841 gcgtgggaaa tcccccggga gtcgctgcgg ctggaggtga agctggggca gggctgcttt
      901 ggagaggtct ggatggggac ctggaacggc accaccagag tggccataaa gactctgaag
      961 cccggcacca tgtccccgga ggccttcctg caggaagccc aagtgatgaa gaagctccgg
     1021 catgagaagc tggttcagct gtacgcagtg gtgtcggaag agcccatcta catcgtcact
     1081 gagtacatga gcaaggggag cctcctggat ttcctgaagg gagagatggg caagtacctg
     1141 cggctgccac agctcgtcga tatggctgct cagattgcat ccggcatggc ctatgtggag
     1201 aggatgaact acgtgcaccg agacctgcgg gcggccaaca tcctggtggg ggagaacctg
     1261 gtgtgcaagg tggctgactt tgggctggca cgcctcatcg aggacaacga gtacacagca
     1321 cggcaaggtg ccaagttccc catcaagtgg acagcccccg aggcagccct ctatggccgg
     1381 ttcaccatca agtcggatgt ctggtccttc ggcatcctgc tgactgagct gaccaccaag
     1441 ggccgggtgc catacccagg gatggtcaac agggaggtgc tggaccaggt ggagaggggc
     1501 taccgcatgc cctgcccgcc cgagtgcccc gagtcgctgc atgacctcat gtgccagtgc
     1561 tggcggaagg accctgagga gcggcccact tttgagtacc tgcaggcctt cctggaggac
     1621 tacttcacct cgacagagcc ccagtaccag cctggagaga acctacggga tccaccggtc
     1681 gccaccatga gtgcgattaa gccagacatg aagatcaaac tccgtatgga aggcaacgta
     1741 aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga gggaaaacag
     1801 agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc
     1861 actgcattcc attacggcaa cagggtattc gccaaatatc cagacaacat acaagactat
     1921 tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg
     1981 ggcatttgca ttgccagaaa cgacataaca atggaagggg acactttcta taataaagtt
     2041 cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa
     2101 tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattcat
     2161 atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa
     2221 gctaaggaga agggtgtcaa gttaccaggc taccactttg tggaccactg cattgagatt
     2281 ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct
     2341 ggattgcctg acaatgccag acgataagcg gccgcgactc tagatcataa tcagccatac
     2401 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2461 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2521 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2581 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     2641 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     2701 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2761 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2821 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2881 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2941 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3001 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3061 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3181 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     3241 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3301 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3361 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3421 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3481 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3541 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     3601 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3661 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3721 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3781 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3841 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3901 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3961 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4021 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4081 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     4141 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4201 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4261 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4321 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4381 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4441 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4501 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4561 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4621 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     4681 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4741 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4801 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4861 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4921 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5101 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5581 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5641 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     5701 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5761 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5821 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5881 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     5941 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6001 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     6061 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6121 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6181 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6241 tacggtggga ggtctatata agcagagct
//
