LOCUS       sequence-21315-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCALSL-mir30
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    175..256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    402..483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    526..660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(669..702)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attaatttcg agcgaacgcg tactagtata acttcgtata gcatacatta tacgagttat
       61 tttttgagct ccataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      121 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atccgcggca
      301 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      361 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      421 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      481 tgcattctag ttgtggtttg tccaaactca tcaatgtata tcgataactt gtttattgca
      541 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      601 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      661 aaatccgaat aacttcgtat agcatacatt atacgaagtt atactagtct cgatcgacta
      721 gggataacag ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
      781 atctt
//
