LOCUS       sequence-213150-        7368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7368)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Desmocollin1-C-18
FEATURES             Location/Qualifiers
     source          1..7368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    4096..4217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4133..4152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4187..4206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4224..4679)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4361..4382)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4573..4592
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4706..4810
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4812..5169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4812..5003
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4835..4855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5020..5155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5204..5998
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5204..5998
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5258..5277)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5868..5887
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6186..6205)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6230..6277
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6606..7194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7095..7114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
     1021 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggactca gatctagtag cggaggtagc
     1261 gcatccggcg gaagcggagg aagcgctctg gcctctgctg ccccagggag catcttctgt
     1321 aagcagctcc ttttctctct cctggtttta acattacttt gcgatgcttg tcagaaagtt
     1381 tatcttcgag ttccttctca tcttcaggct gaaacacttg taggcaaagt gaatctggag
     1441 gagtgtctca agtcggccag cctaatccgg tccagtgacc ctgccttcag gattctagaa
     1501 gatggctcaa tttacacaac acatgacctc attttgtctt ctgaaaggaa aagtttttcc
     1561 attttccttt cagatggtca gagacgggaa caacaagaga taaaagttgt actgtcagca
     1621 agagaaaaca agtctcctaa gaagagacat accaaagaca cagccctcaa gcgcagcaag
     1681 agacgatggg ctcctattcc agcttcattg atggagaact cgttgggtcc atttccacaa
     1741 cacgttcagc agatccaatc tgatgctgca cagaattaca ccatctttta ttccataagt
     1801 gggccaggcg tggacaaaga acccttcaat ttgttttaca tagagaaaga cactggggat
     1861 atcttttgta caaggagcat tgaccgtgag aaatacgaac agtttgcgtt atatggctat
     1921 gcaacaactg cagatggcta tgcaccagaa tatccactcc ctttgatcat caaaattgaa
     1981 gatgataatg ataacgcccc atattttgaa cacagagtga ctatctttac tgtgcctgaa
     2041 aattgccgat ccggcacttc agtgggaaaa gtgaccgcca cagaccttga cgaacctgac
     2101 actctccata ctcgtctgaa atataaaatc ttacaacaaa tcccagatca tccaaagcat
     2161 ttctccatac acccagatac cggagtcatc accacaacta caccttttct ggatagagaa
     2221 aaatgtgata cttaccagtt aataatggaa gtgcgagaca tgggtggtca gcctttcggt
     2281 ttatttaata caggaacaat tactatttca cttgaggatg aaaatgacaa tccaccatct
     2341 ttcacagaaa cttcttatgt tacagaagta gaagaaaaca gaattgacgt ggagattttg
     2401 cgaatgaagg tacaggatca ggatttgcca aacactcctc actcaaaggc tgtatacaaa
     2461 atcttacaag gaaatgaaaa tggaaacttc ataattagca cagatccaaa tacaaatgaa
     2521 ggagtgctgt gtgttgtcaa gccattgaac tatgaagtca atcgccaagt tattttgcaa
     2581 gttggtgtca ttaacgaggc acaattctct aaagcagcga gctcacaaac tcctacaatg
     2641 tgcactacaa ctgtcaccgt taaaattata gacagtgatg agggccctga atgccaccct
     2701 ccagtgaaag ttattcagag tcaagatggc ttcccagctg gccaagaact ccttggatac
     2761 aaagcactgg acccggaaat atccagtggt gaaggcttaa ggtatcagaa gttaggggat
     2821 gaagataact ggtttgaaat taatcaacac actggcgact tgagaactct aaaagtacta
     2881 gatagagaat ccaaatttgt aaaaaacaac caatacaata tttcagttgt tgcagtggat
     2941 gcagttggcc gatcttgcac tggaacatta gtagttcatt tggatgatta caacgatcac
     3001 gcacctcaaa ttgacaaaga agtgaccatt tgtcagaata atgaggattt tgctgttctg
     3061 aaacctgtag atccagatgg acctgaaaat ggaccacctt ttcaattctt tctggataat
     3121 tctgccagta aaaactggaa catagaagaa aaggatggta aaactgccat tcttcgtcaa
     3181 cggcaaaatc ttgattataa ctattattct gtgcctattc aaataaaaga caggcatggt
     3241 ttagttgcaa cacatatgtt aacagtgaga gtatgtgact gttcaactcc atctgagtgt
     3301 agaatgaagg ataaaagtac aagagacgtt agaccaaatg taatacttgg aagatgggct
     3361 attcttgcta tggtgttggg ttctgtattg ttattatgta ttctgtttac atgtttctgt
     3421 gtcactgcta agagaacagt caagaaatgt tttccagaag acatagccca gcaaaattta
     3481 attgtatcaa atactgaagg acctggagaa gaagtaacgg aagcaaatat tagactcccc
     3541 atgcagacat ccaacatttg tgacacaagc atgtctgttg gtactgttgg tggccaggga
     3601 atcaaaacac agcaaagttt tgagatggtc aaaggaggct acactttgga ttccaacaaa
     3661 ggaggtggac atcagacctt ggagtccgtc aagggagtgg ggcagggaga tactggcaga
     3721 tatgcgtaca cggactggca gagtttcacc caacctcggc ttggcgaaaa ggtgtatttg
     3781 tgtggacaag atgaggagca taaacattgt gaagactacg tttgttcgta taactatgaa
     3841 ggcaaaggtt ctctggccgg ctcagtaggt tgctgcagcg atcggcagga agaagaggga
     3901 ctggagtttc tagatcacct ggaacccaaa tttaggacat tagcaaagac atgcatcaag
     3961 aaataaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     4021 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4081 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4201 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4261 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4321 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4381 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4441 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4501 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4561 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4621 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4741 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4801 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4861 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4921 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4981 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5041 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5101 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5161 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5221 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5281 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5341 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5401 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5461 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5521 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5581 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5641 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5701 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5761 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5821 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5881 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5941 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6001 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6061 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6121 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6181 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6241 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6301 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6361 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6421 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6601 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6661 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6721 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6781 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6841 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6901 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6961 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7021 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7081 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7141 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7201 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7261 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     7321 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
