LOCUS       sequence-213154-        5530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5530)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos-A69V-EB3-7
FEATURES             Location/Qualifiers
     source          1..5530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      282..291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1152..1832
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    1954..2075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1991..2010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2045..2064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2082..2537)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2219..2240)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2431..2450
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2564..2668
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2670..3027
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2670..2861
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2693..2713)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2878..3013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3062..3856
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3062..3856
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3116..3135)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3726..3745
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4044..4063)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4088..4135
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4464..5052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4953..4972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5227..5530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttcgaattc tgcagtcgac tgccaccatg gccgtcaatg
      301 tgtactccac atctgtgacc agtgaaaatc tgagtcgcca tgatatgctt gcatgggtca
      361 acgactccct gcacctcaac tataccaaga tagaacagct ttgttcaggg gcagcctact
      421 gccagttcat ggacatgctc ttccccggct gtgtgcactt gaggaaagtg aagttccagg
      481 ccaaactaga gcatgaatac atccacaact tcaaggtgct gcaagcagct ttcaagaaga
      541 tgggtgttga caaaatcatt cctgtagaga aattagtgaa aggaaaattc caagataatt
      601 ttgagtttat tcagtggttt aagaaattct ttgacgcaaa ctatgatgga aaggattaca
      661 accctctgct ggcgcggcag ggccaggacg tagcgccacc tcctaaccca ggtgatcaga
      721 tcttcaacaa atccaagaaa ctcattggca cagcagttcc acagaggacg tcccccacag
      781 gcccaaaaaa catgcagacc tctggccggc tgagcaatgt ggcccccccc tgcattctcc
      841 ggaagaatcc tccatcagcc cgaaatggcg gccatgagac tgatgcccaa attcttgaac
      901 tcaaccaaca gctggtggac ttgaagctga cagtggatgg gctggagaag gaacgtgact
      961 tctacttcag caaacttcgt gacatcgagc tcatctgcca ggagcatgaa agtgaaaaca
     1021 gccctgttat ctcaggcatc attggcatcc tctatgccac agaggaagga ttcgcacccc
     1081 ctgaggacga tgagattgaa gagcatcaac aagaagacca ggacgagtac ctggatccac
     1141 cggtcgccac catgagtgcg attaagccag acatgaagat caacctccgt atggaaggca
     1201 acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct tttgagggaa
     1261 aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc
     1321 tgaccactgc attccattac ggcaacaggg tattcgtcga atatccagac cacatacaag
     1381 actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag
     1441 acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact ttctataata
     1501 aagttcgatt tcacggtaca aactttcccg ccaatggtcc agttatgcag aagaagacgc
     1561 tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg acgggtgata
     1621 ttcacatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt
     1681 acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac cactgcattg
     1741 agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc
     1801 attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc
     1861 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1921 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1981 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2041 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     2101 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2161 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2221 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2281 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2341 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2401 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2461 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2521 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     2701 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2761 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     2821 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2941 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3001 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     3061 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3121 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3181 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3241 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3301 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3361 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3421 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3481 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3541 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     3601 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3661 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3721 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3781 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3841 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     3901 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3961 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4021 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4081 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4141 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     4201 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     4261 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     4321 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4681 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4741 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4801 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4861 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4921 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4981 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5041 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5101 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5161 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     5281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5521 tattaccatg
//
