LOCUS       sequence-213160-        6447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6447)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos-M-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..6447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      537..546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1873..1884
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2373..3053
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3175..3296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3212..3231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3266..3285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3303..3758)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3440..3461)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3652..3671
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3785..3889
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3891..4248
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3891..4082
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3914..3934)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4099..4234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4283..5077
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4283..5077
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4337..4356)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4947..4966
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5265..5284)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5309..5356
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5685..6273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6174..6193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgggtcg ggggctgctc aggggcctgt ggccgctgca catcgtcctg tggacgcgta
      601 tcgccagcac gatcccaccg cacgttcaga agtcggatgt ggaaatggag gcccagaaag
      661 atgaaatcat ctgccccagc tgtaatagga ctgcccatcc actgagacat attaataacg
      721 acatgatagt cactgacaac aacggtgcag tcaagtttcc acaactgtgt aaattttgtg
      781 atgtgagatt ttccacctgt gacaaccaga aatcctgcat gagcaactgc agcatcacct
      841 ccatctgtga gaagccacag gaagtctgtg tggctgtatg gagaaagaat gacgagaaca
      901 taacactaga gacagtttgc catgacccca agctccccta ccatgacttt attctggaag
      961 atgctgcttc tccaaagtgc attatgaagg aaaaaaaaaa gcctggtgag actttcttca
     1021 tgtgttcctg tagctctgat gagtgcaatg acaacatcat cttctcagaa gaatataaca
     1081 ccagcaatcc tgacttgttg ctagtcatat ttcaagtgac aggcatcagc ctcctgccac
     1141 cactgggagt tgccatatct gtcatcatca tcttctactg ctaccgcgtt aaccggcagc
     1201 agaagctgag ttcaacctgg gaaaccggca agacgcggaa gctcatggag ttcagcgagc
     1261 actgtgccat catcctggaa gatgaccgct ctgacatcag ctccacgtgt gccaacaaca
     1321 tcaaccacaa cacagagctg ctgcccattg agctggacac cctggtgggg aaaggtcgct
     1381 ttgctgaggt ctataaggcc aagctgaagc agaacacttc agagcagttt gagacagtgg
     1441 cagtcaagat ctttccctat gaggagtatg cctcttggaa gacagagaag gacatcttct
     1501 cagacatcaa tctgaagcat gagaacatac tccagttcct gacggctgag gagcggaaga
     1561 cggagttggg gaaacaatac tggctgatca ccgccttcca cgccaagggc aacctacagg
     1621 agtacctgac gcggcatgtc atcagctggg aggacctgcg caagctgggc agctccctcg
     1681 cccgggggat tgctaacctc cacagtgatc acactccatg tgggaggccc aagatgccca
     1741 tcgtgcacag ggacctcaag agctccaata tcctcgtgaa gaacgaccta acctgctgcc
     1801 tgtgtgactt tgggctttcc ctgcgtctgg accctactct gtctgtggat gacctggcta
     1861 acagtgggca ggtgggaact gcaagataca tggctccaga agtcctagaa tccaggatga
     1921 atttggagaa tgttgagtcc ttcaagcaga ccgatgtcta ctccatggct ctggtgctct
     1981 gggaaatgac atctcgctgt aatgcagtgg gagaagtaaa agattatgag cctccatttg
     2041 gttccaaggt gcgggagcac ccctgtgtcg aaagcatgaa ggacaacgtg ttgagagatc
     2101 gagggcgacc agaaattccc agcttctggc tcaaccacca gggcatccag atggtgtgtg
     2161 agacgttgac tgagtgctgg gaccacgacc cagaggcccg tctcacagcc cagtgtgtgg
     2221 cagaacgctt cagtgagctg gagcatctgg acaggctctc ggggaggagc tgctcggagg
     2281 agaagattcc tgaagacggc tccctaaaca ctaccaaaag cagcggtgga gcaagcgcag
     2341 ccagtggtag cgcggatcca ccggtcgcca ccatgagtgc gattaagcca gacatgaaga
     2401 tcaaactccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta
     2461 caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc
     2521 tgccttttgc ctttgatatc ctgaccactg cattcatgta cggcaacagg gtattcgcca
     2581 aatatccaga caacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg
     2641 aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg
     2701 aaggggacac tttctataat aaagttcgat tttatggtac caactttccc gccaatggtc
     2761 cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg
     2821 atggagtgct gacgggtgat attcatatgg ctttgttgct tgaaggaaat gcccattacc
     2881 gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc
     2941 actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc
     3001 tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga taagcggccg
     3061 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3121 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     3301 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3361 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3421 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3481 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3541 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3601 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3661 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3721 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3841 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3901 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3961 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4081 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4141 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4201 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     4261 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4321 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4381 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4441 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4501 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4561 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4621 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4681 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4741 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4801 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4861 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4921 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4981 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5041 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5101 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5161 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5221 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     5281 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     5341 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5401 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5461 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5521 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5581 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5641 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5701 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5761 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5821 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5881 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5941 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6001 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6061 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6121 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6181 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6241 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6301 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6361 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     6421 agttcatagc ccatatatgg agttccg
//
