LOCUS       sequence-213165-        5446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission:
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-CD151-7
FEATURES             Location/Qualifiers
     source          1..5446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    1870..1991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1907..1926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1961..1980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1998..2453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2135..2156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2347..2366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2480..2584
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2586..2943
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2586..2777
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2609..2629)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2794..2929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2978..3772
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2978..3772
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3032..3051)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3642..3661
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3960..3979)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4004..4051
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4380..4968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4869..4888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5143..5446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg
      301 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
      361 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
      421 ccactgcatt ccattacggc aacagggtat tcgccaaata tccagacaac atacaagact
      481 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
      541 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
      601 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
      661 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc
      721 atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
      781 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
      841 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
      901 ctggattgcc tgacaatgcc agacgatccg gactcagatc tggcggtatg ggtgagttca
      961 acgagaagaa gacaacatgt ggcaccgttt gcctcaagta cctgctgttt acctacaatt
     1021 gctgcttctg gctggctggc ctggctgtca tggcagtggg catctggacg ctggccctca
     1081 agagtgacta catcagcctg ctggcctcag gcacctacct ggccacagcc tacatcctgg
     1141 tggtggcggg cactgtcgtc atggtgactg gggtcttggg ctgctgcgcc accttcaagg
     1201 agcgtcggaa cctgctgcgc ctgtacttca tcctgctcct catcatcttt ctgctggaga
     1261 tcatcgctgg tatcctcgcc tacgcctact accagcagct gaacacggag ctcaaggaga
     1321 acctgaagga caccatgacc aagcgctacc accagccggg ccatgaggct gtgaccagcg
     1381 ctgtggacca gctgcagcag gagttccact gctgtggcag caacaactca caggactggc
     1441 gagacagtga gtggatccgc tcacaggagg ccggtggccg tgtggtccca gacagctgct
     1501 gcaagacggt ggtggctctt tgtggacagc gagaccatgc ctccaacatc tacaaggtgg
     1561 agggcggctg catcaccaag ttggagacct tcatccagga gcacctgagg gtcattgggg
     1621 ctgtggggat cggcattgcc tgtgtgcagg tctttggcat gatcttcacg tgctgcctgt
     1681 acaggagtct caagctggag cactactgag aattctgcag tcgacggtac cgcgggcccg
     1741 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     1801 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     1861 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1981 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     2041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     2101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     2161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     2221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     2461 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2521 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2581 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     2641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     2701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2761 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2821 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     2881 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     2941 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     3001 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     3061 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3121 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     3181 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3241 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3301 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3361 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3421 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3481 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     3541 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3601 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3661 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3721 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     3781 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     3841 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     3901 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     3961 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     4021 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     4081 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     4141 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4201 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     4261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4501 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4741 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5041 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     5101 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     5161 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     5221 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     5281 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     5341 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     5401 tcctacttgg cagtacatct acgtattagt catcgctatt accatg
//
