LOCUS       sequence-213168-       10220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane/Actin, Excitation: 505 / 569, Emission: 516 /
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Supervillin-45
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    6948..7069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6985..7004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7039..7058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7076..7531)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7213..7234)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7425..7444
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7558..7662
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7664..8021
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7664..7855
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7687..7707)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7872..8007
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8056..8850
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8056..8850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8110..8129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8720..8739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9038..9057)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9082..9129
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9458..10046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9947..9966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
     1021 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggactca gatctcgagc tcaagcttcg
     1261 aattctgcag tcgacggtac cttcgatgct cagcttttag ctgcccttcc taaagttgca
     1321 gaattaagaa aaatctttga accaaagagg aaagaatttt tagaaatgaa aagaaaagaa
     1381 agaattgccc ggcgcttaga aggaattgaa accgacacgc agcccatcct cttgcagagc
     1441 tgcacgggct tggtgactca tcgcctgctg gaggaggaca cacccagata catgcgtgcc
     1501 acagacccgg ccagcccgca cattggtcga tcaaatgaag aagaagaaac ttcagattct
     1561 tcactagaaa agcagactcg atccaaacag tgcacagaaa cctcaggcat ccatgctgac
     1621 tcgccctaca gttcaggcat catggacacc cagagcctcg agtccaaagc cgaaagaatc
     1681 gccaggtaca aagcagagag aagacggcag ctggcagaaa agtatgggct caccctggat
     1741 ccggaagcag actctgaaac tccatctcga tacagcaggt ccagaaagga ccccgaggct
     1801 gcggagaaaa ggggagtaag aagcgagcga tcggccgagt ccagcagaga cgccggctca
     1861 tcctactcca ggactgagct ctcggggctc aggacctgcg tggctgagtc caaggactac
     1921 ggcctgcacc ggagtgacgg tgtttccgac acagaggtgc tgctcaatgc agaaaaccaa
     1981 agacgcggtc aagagcccag tgccaccggg ctggcccgag acctgcccct tgcaggggag
     2041 gtctcctctt ctttctcatt ctccgggcga gactctgccc tcggtgaagt gccaaggtcc
     2101 ccaaaggcag tgcacagcct gcccagtcca tcgcctgggc agccggcctc cccaagccac
     2161 tccaccagtg acctgccact ccctgcggag gctcgagcca ggtcaacttc aaattcagaa
     2221 atgccagctg ccgaggatga agaaaaggtc gatgagcgag cgaggctgag cgtcgctgct
     2281 aagaggctgc tcttcaggga gatggagaag tcgtttgacg agaagagcgt cccaaagcgc
     2341 cgctcccgca atgcggccgt ggagcagagg ctgcggcgcc tccaggaccg gtcccacacg
     2401 cagcccgtca ccaccgagga ggtggtcatt gcagccacat tgcaggcgtc tgctcaccaa
     2461 aaggcactag ccagagacca gacaaacgag agcaaagatt ctgctgagca gggagaaccc
     2521 gactcctcca ctctgagctt agcggagaag ctggccttgt ttaacaaact atctcagccg
     2581 gtctcaaaag ccatttcaac acggaacaga ttagacatga gacagaggag gatgaatgct
     2641 cgttatcaaa cccagccggt cacgcttgga gaagtggaac aggtgcagag tggcaagctc
     2701 atggctttct ctcccaccat taacacttcc gtgtccaccg tggcatctac cgtccccccc
     2761 atgtatgcag gaaatcttcg gacaaagcca cttccagatg acagctttgg tgccactgag
     2821 cagaagtttg cttcttcgct agaaaactcc gactccccag ttagaagcat cctgaaatcc
     2881 caaggctggc agccctcggt cgagggtgct gggagcaaag caatgttgag agaatttgaa
     2941 gagacagaac gcaagggagg cttgacaggt ggagatggcg gggttacaaa gtatgggtcc
     3001 ttcgaggaag cagagctgtc ctaccccgtc ctcagcagag tccgggaggg agacaaccat
     3061 aaagaggcca tctatgccct tccgaggaaa ggaagcctgg agctcgccca tcctcccatc
     3121 gcccagcttg gtgatgacct gaaggaattt tccaccccaa agagcaccat gcaggcaagc
     3181 ccagactgga aggagaggca gctctttgaa gagaaggtgg acttggagaa tgtcacaaag
     3241 aggaagtttt cgctgaaagc cgcggagttc ggggaaccca cttctgagca gaccggcgcg
     3301 gctgctggga aaccggctgc tccgactgca accccggtgt cctggaagcc gcaggatccc
     3361 tccgaacagc ctcaggagaa gcggtatcag agcccgtgtg cgatgtttgc tgctggagag
     3421 atcaaagccc cggcggtgga gggcagcctc gactccccca gcaaaaccat gtccattaaa
     3481 gaaagattag cactgttgaa gaagagcggt gaggaagact ggagaaacag actcaacaga
     3541 aagcaggaat acggcaaagc ctccatcacc agcagcctgc acatccaaga gacggagcag
     3601 tcactcaaga agaagcgggt cacagaaagt cgagagagcc agatgactat tgaagagaga
     3661 aagcatctca tcaccgtgag agaggacgcc tggaagacca ggggcaaagg agcagccaac
     3721 gactctaccc agtttactgt ggccggcagg atggtgaagc gaggtctggc gtcccccact
     3781 gccatcaccc cggtagcatc ccctgtttcc agcaaagcaa ggggcactac accagtttcc
     3841 agacccctgg aagatatcga agccagacca gacatgcagt tagagtcgga cctcaagctg
     3901 gacaggctgg aaaccttcct aagaaggctg aataacaaag ttggtgggat gcaagaaaca
     3961 gtcctcactg tcactgggaa atcggtgaaa gaggtgatga agccggacga tgatgaaacc
     4021 tttgccaagt tttaccgcag cgtggactcc tctctaccga gaagccccgt agagctggac
     4081 gaggatttcg atgtcatttt tgatccttat gcccccaggt tgacatcctc ggtggctgag
     4141 cacaagcgtg ccgtgcggcc caagcgccgg gtccaggctt ccaaaaaccc cctgaagatg
     4201 ctggccgcga gggaagatct cctgcaggag tacactgagc agagactgaa cgtggccttc
     4261 gtggagtcaa agcggatgaa agtcgaaaag ttgtccgcca actccagctt ctcagaagtc
     4321 accctggcgg ggttagccag caaagaaaac ttcagcaacg tcagcctgcg gagcgtcaac
     4381 ctgacggaac agaattccaa caacagtgca gtgccctaca agaagctgat gctgctgcag
     4441 gttaaaggaa gaagacacgt gcagacgcgg ctggtggagc ctcgcgcccc ctccctcaac
     4501 agcggggact gcttcctcct gctctcgccc catcactgct tcctgtgggt cggagagttc
     4561 gccaacgtga tcgagaaggc gaaggcctca gaacttgcga gtttaattca gaccaagagg
     4621 gaacttggtt gtagagcaac ttacatccag actgttgaag aaggaattaa tacacacaca
     4681 catgcagcca aagacttctg gaagcttctg ggcggccaag ccagttacca atccgccgga
     4741 gacccaaagg aggatgagct ctatgaaacg gccataatag agaccaactg catttaccgt
     4801 ctgatggatg acaaactcgt tcctgatgat gactactggg ggaagatccc aaagtgctcc
     4861 ctcctgcaat caaaagaggt actggtgttt gattttggga gtgaagttta cgtgtggcat
     4921 ggaaaagaag tcacattagc acaacggaaa atcgcctttc agctggcaaa gcacttgtgg
     4981 aatggaacct ttgactacga gaattgtgac attaacccgc tggatcctgg ggaatgcaat
     5041 cccctcattc ccaggaaagg acaggggcgg cccgactggg caatatttgg gagacttaca
     5101 gaacacaatg agacgatctt gttcaaagag aaattccttg attggacgga actgaagaga
     5161 cctaacgaga agaacgccag cgaactcgcg cagcacaagg acgatgcccg ggcagaggtc
     5221 aagccttacg acgtgacgcg gatggtgccc gtgccccaga caacagccgg caccgtgctg
     5281 gatggggtca acgtgggccg aggctacggg ctggtggaag gggacgaccg gaggcagttc
     5341 gagatcgcca gcatctcagt ggacgtctgg cacatcctgg agttcgacta cagcaggctc
     5401 cccaagcaga gcatcgggca gttccacgaa ggcgatgcct acgtggtgaa gtggaagttc
     5461 atcgtgagca ctgcagtggg aagccggcag aagggggagc actcggtcag ggtggctggc
     5521 aaagagaaat gtgtctactt cttctggcaa ggccgccagt cgaccgtgag tgagaagggc
     5581 acatcagctc tgatgacagt ggagctggac gaggaaaggg gggcccaggt ccaggtcctg
     5641 cagggcaagg agcccccctg tttcctgcag tgcttccagg gggggatggt ggtccactcc
     5701 gggagacggg aggaggagga agagaacaca cagagcgagt ggcggctgta ctgtgtgcgc
     5761 ggagaggtgc ctgtggaggg gaacttgctg gaggtggcct gtcactgcag cagcctcagg
     5821 tctaggacgt ccatggttgt cctcaacgtc cacaaagccc tcatctacct gtggcatgga
     5881 tgcaaagccc aggcccacac gaaggaggtc ggacggactg cagccaataa aatcaaggac
     5941 caatgtcccc tggaggccgg gctgcacagt agcagcaaag tgacaattca tgagtgtgat
     6001 gaaggctcgg agcccctggg gttctgggac gctctgggga ggagagaccg gaaagcctac
     6061 gactgcatgc ttcaagatcc tggaaatttt aacttcacac cccgcctgtt catcctcagc
     6121 agctcctctg gtgacttctc agccacagag ttcatgtatc ccgcccgaga cccctctgtg
     6181 gtcaactcta tgcccttcct gcaggaagac ctatacagtg ccccacagcc agcactcttc
     6241 cttgttgaca atcaccacga ggtgtacctc tggcaaggct ggtggcccat cgagaacaag
     6301 atcacgggct ccgcgcgcat ccgctgggcc tcggaccgga agagcgccat ggagacagtg
     6361 ctgcagtact gccgagggaa aaacctgaag aagccgcccc ccaagtctta cctcatccac
     6421 gctggcctgg aacccctgac cttcaccaac atgttcccca gctgggagca cagagaagac
     6481 attgcagaga tcacggagat ggacacggaa gtttcaaatc agatcaccct agtggaggat
     6541 gtcttagcca aactgtgtaa gaccatttac ccactggccg acctcctagc caggccgctc
     6601 cctgagggag ttgaccctct gaagctggag atctatctca ccgacgagga cttcgagttt
     6661 gcactagaca tgacccgaga cgagtacaat gcactgcccg cttggaagca ggtgaacctg
     6721 aagaaagcga aaggtctgtt ctgagtcagg agatgctgga ggaacctggg tggtcacttt
     6781 caatactgat ggaccaggaa aatggatgtt taatcgaatt cccgcggcct tctagataac
     6841 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6901 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
     7081 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7141 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     7201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     7261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     7321 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7501 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     7561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     7681 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7741 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7801 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7861 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7921 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7981 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     8041 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     8101 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     8161 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     8221 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     8281 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     8341 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     8401 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     8461 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     8521 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     8581 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     8641 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     8701 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     8761 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     8821 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     8881 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     8941 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     9001 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     9061 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     9121 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     9181 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     9241 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     9301 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9361 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9421 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9481 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9541 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9601 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9661 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9721 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9781 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9841 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9901 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9961 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10021 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10081 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10141 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
    10201 agcccatata tggagttccg
//
